Title:
METHOD AND KIT FOR DETECTING 5-HYDROXYMETHYLCYTOSINE IN NUCLEIC ACIDS
Document Type and Number:
WIPO Patent Application WO/2012/141324
Kind Code:
A1
Abstract:
Provided are a method and a kit for detecting 5-hydroxymethylcytosine in nucleic acids. The method for detecting 5-hydroxymethylcytosine in nucleic acids comprises the following steps: (1) a step for oxidation of 5-hydroxymethylcytosine by treating a nucleic acid sample with peroxytungstic acid, tungstic acid, a salt thereof or a combination of the same and a reoxidant; and (2) a step for determining the position of the oxidized 5-hydroxymethylcytosine in the nucleic acid sample.
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Inventors:
OKAMOTO AKIMITSU (JP)
SUGIZAKI KAORI (JP)
NAKAMURA AKIKO (JP)
YANAGISAWA HIROYUKI (JP)
IKEDA SHUJI (JP)
SUGIZAKI KAORI (JP)
NAKAMURA AKIKO (JP)
YANAGISAWA HIROYUKI (JP)
IKEDA SHUJI (JP)
Application Number:
PCT/JP2012/060265
Publication Date:
October 18, 2012
Filing Date:
April 16, 2012
Export Citation:
Assignee:
RIKEN (JP)
OKAMOTO AKIMITSU (JP)
SUGIZAKI KAORI (JP)
NAKAMURA AKIKO (JP)
YANAGISAWA HIROYUKI (JP)
IKEDA SHUJI (JP)
OKAMOTO AKIMITSU (JP)
SUGIZAKI KAORI (JP)
NAKAMURA AKIKO (JP)
YANAGISAWA HIROYUKI (JP)
IKEDA SHUJI (JP)
International Classes:
C12Q1/68; C12N15/09
Domestic Patent References:
| WO2006132022A1 | 2006-12-14 | |||
| WO2007111324A1 | 2007-10-04 |
Other References:
TAHILIANI M ET AL.: "Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in mammalian DNA by MLL partner TET1.", SCIENCE, vol. 324, no. 5929, 15 May 2009 (2009-05-15), pages 930 - 935, XP002545640
ITO S ET AL.: "Role of Tet proteins in 5mC to 5hmC conversion, ES-cell self-renewal and inner cell mass specification.", NATURE, vol. 466, no. 7310, 26 August 2010 (2010-08-26), pages 1129 - 1133, XP055092131
MUNZEL M ET AL.: "Quantification of the Sixth DNA Base Hydroxymethylcytosine in the Brain", ANGEW. CHEM. INT. ED., vol. 49, no. 31, 19 July 2010 (2010-07-19), pages 5375 - 5377, XP055057746
SONG CX ET AL.: "Selective chemical labeling reveals the genome-wide distribution of 5- hydroxymethylcytosine.", NAT. BIOTECHNOL., vol. 29, no. 1, 12 December 2010 (2010-12-12), pages 68 - 72, XP055012325, Retrieved from the Internet [retrieved on 20120426]
KAMATA K ET AL.: "Hydrogen-bond-assisted epoxidation of homoallylic and allylic alcohols with hydrogen peroxide catalyzed by selenium- containing dinuclear peroxotungstate.", J. AM. CHEM. SOC., vol. 131, no. 20, 27 May 2009 (2009-05-27), pages 6997 - 7004, XP055092204
RIKEN: "Tungsten Sankazai o Tsukai, Saibo Kino Shokika no Mechanism Kaimei e", 30 August 2011 (2011-08-30), JAPAN, XP008171821, Retrieved from the Internet [retrieved on 20120426]
OKAMOTO A ET AL.: "5-Hydroxymethylcytosine- selective oxidation with peroxotungstate.", CHEM. COMMUN., vol. 47, no. 40, 28 October 2011 (2011-10-28), pages 11231 - 11233, XP055092209
SCIENCE, vol. 324, no. 5929, 2009, pages 930 - 935
NATURE, vol. 466, 2010, pages 1129 - 1133
ANGEW. CHEM. INT. ED., vol. 49, 2010, pages 5375 - 5377
NATURE BIOTECHNOL., vol. 29, 2011, pages 68 - 72
ITO S ET AL.: "Role of Tet proteins in 5mC to 5hmC conversion, ES-cell self-renewal and inner cell mass specification.", NATURE, vol. 466, no. 7310, 26 August 2010 (2010-08-26), pages 1129 - 1133, XP055092131
MUNZEL M ET AL.: "Quantification of the Sixth DNA Base Hydroxymethylcytosine in the Brain", ANGEW. CHEM. INT. ED., vol. 49, no. 31, 19 July 2010 (2010-07-19), pages 5375 - 5377, XP055057746
SONG CX ET AL.: "Selective chemical labeling reveals the genome-wide distribution of 5- hydroxymethylcytosine.", NAT. BIOTECHNOL., vol. 29, no. 1, 12 December 2010 (2010-12-12), pages 68 - 72, XP055012325, Retrieved from the Internet
KAMATA K ET AL.: "Hydrogen-bond-assisted epoxidation of homoallylic and allylic alcohols with hydrogen peroxide catalyzed by selenium- containing dinuclear peroxotungstate.", J. AM. CHEM. SOC., vol. 131, no. 20, 27 May 2009 (2009-05-27), pages 6997 - 7004, XP055092204
RIKEN: "Tungsten Sankazai o Tsukai, Saibo Kino Shokika no Mechanism Kaimei e", 30 August 2011 (2011-08-30), JAPAN, XP008171821, Retrieved from the Internet
OKAMOTO A ET AL.: "5-Hydroxymethylcytosine- selective oxidation with peroxotungstate.", CHEM. COMMUN., vol. 47, no. 40, 28 October 2011 (2011-10-28), pages 11231 - 11233, XP055092209
SCIENCE, vol. 324, no. 5929, 2009, pages 930 - 935
NATURE, vol. 466, 2010, pages 1129 - 1133
ANGEW. CHEM. INT. ED., vol. 49, 2010, pages 5375 - 5377
NATURE BIOTECHNOL., vol. 29, 2011, pages 68 - 72
Attorney, Agent or Firm:
TSUJII Koichi et al. (JP)
辻居 Koichi (JP)
辻居 Koichi (JP)
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