Title:
METHOD AND KIT FOR NON-INVASIVELY DETECTING EGFR GENE MUTAIONS
Document Type and Number:
WIPO Patent Application WO/2015/101175
Kind Code:
A1
Abstract:
The present invention discloses a method for non-invasively detecting EGFR gene mutations in subjects, comprising the following steps: designing primers according to EGFR gene exons; extracting plasma DNAs in subjects; connecting the extracted plasma DNAs with tagging linkers; PCR pre-amplifying the tagging linkers connected plasma DNAs; cyclising the pre-amplified DNAs to obtain cyclised DNAs; PCR amplifying the cyclised DNAs using the designed primers; and high throughput sequencing the PCR amplified product and analyzing the EGFR gene mutations. The present invention also discloses a corresponding kit.
Inventors:
WANG LINA (CN)
RU LANLAN (CN)
LI TIANCHENG (CN)
ZHANG YAXI (CN)
ZHANG JIANGUANG (CN)
RU LANLAN (CN)
LI TIANCHENG (CN)
ZHANG YAXI (CN)
ZHANG JIANGUANG (CN)
Application Number:
PCT/CN2014/093940
Publication Date:
July 09, 2015
Filing Date:
December 16, 2014
Export Citation:
Assignee:
BERRY GENOMICS CO LTD (CN)
International Classes:
C12Q1/68
Foreign References:
| CN102181533A | 2011-09-14 | |||
| CN103298955A | 2013-09-11 | |||
| CN101921830A | 2010-12-22 | |||
| CN101967684A | 2011-02-09 | |||
| CN102851375A | 2013-01-02 |
Other References:
See also references of EP 3090068A4
Attorney, Agent or Firm:
KING & WOOD MALLESONS (East Tower World Financial Centre, No.1 Dongsanhuan Zhonglu,,Chaoyang District, Beijing 0, CN)
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