In recent years, the fabrication of nanochannels has enjoyed much attention because of the increased interest in the manipulation and detection of separate molecules. The de- velopments in the field of optical engineering are forever improving the possibilities of studying biochemical processes taking place on a molecular level. This opens up a vast re- search potential in, for example, the medical and biomedical field. Micro-and nanochannels, may, for example, be used for the separation of biomolecules, enzymatic assays and immuno- hybridisation reactions. An example of the utilisation of mi- cro-and nanochannels is the optical detection of molecules.

In such a case, it is important that at least one side of the channel be transparent to light. For this reason, a great deal of research is performed on the fabrication of nanochan- nels in transparent material. Electrical manipulation of molecules in the nanochannels may also be of interest for re- search. For this purpose, electrodes are applied at both ends of the channels. A good deal of research is therefor also performed on the development of nanochannels that are pro- vided with electrodes.

In the prior art, it is common practice to etch channels into a glass plate or into an insulating intermedi- ate layer of two glass plates and to subsequently bond the two glass plates by means of an adhesive. A drawback of this known method is that in this way the precision of the dimen- sions of the nanochannels is determined by the limited preci-

sion with which the adhesive layer can be applied between the glass plates. This limited precision may be a cause for leaks.

It is also known from the prior art, that after etching the channels, electrodes can be applied by vapour deposition, whereafter the two glass plates are bonded by way of an adhesive. A drawback of this known technique is that the alignment of the electrodes and the channels must be very accurate, which poses a considerable constructural difficulty limiting the. employability of the nanochannels obtained in the known manner. In addition, the application of electrodes by this method may cause local variations in thickness of the intermediate layer, which after bonding of the glass plates may cause leakages.

From US-B 6,517, 736 a microfluid device is known comprising a silicon-wafer and a glass plate, wherein the silicon-wafer is provided with channels, while the wafer also serves as adhesive agent to the glass plate.

It is an object of the present invention to provide a method for the fabrication of nanochannels between a sub- strate and a covering layer, wherein the nanochannels formed are dimensioned very precisely and exhibit no leakages. It is preferred to use conventional techniques for the fabrication.

A further object of the present invention is to pro- vide a method for the accurate placing of electrodes around the above-mentioned nanochannels, which method is easy to carry out, and which in addition does not hinder precise di- mensioning of the nanochannels and does not cause leakages.

Prior to etching the channel into the layer of semi- conductor material, the layer of semiconductor material is in a first aspect of the invention locally doped for the forma- tion of electrodes. With the aid of ion-implantation tech- niques, predetermined sites in the semiconductor material are in this way provided with conductive portions. Subsequently, the channel is etched straight across said conductive por- tions, creating two electrodes at both sides of the channel.

The result of this method is that the two electrodes are per- fectly aligned in relation to each other and in relation to

the channel. Due to the electrodes being applied by doping, the surface of the layer of semiconductor material stays very smooth so as to minimise the occurrence of leakages caused by the fact that the top and bottom layers do not join up.

The semiconductor material is applied to the sub- strate by means of, for example, LPCVD (Low Pressure Chemical Vapour Deposition). As substrate and covering layer it is possible to use, among other things, glass or a semiconductor wafer. However, glass is preferred because glass is transpar- ent to visible light and this allows the products with the nanochannels to be employed for applications in which optical detection methods are used. As semiconductor material any ap- propriate kind of semiconductor may be used. However, amor- phous silicon is preferred because of this material's low deposition rate, which allows the semiconductor material to be applied very accurately in the desired thickness. The thickness of the layer of semiconductor material lies in the order of several tens of nanometers but depending on the ap- plication, the layers may of course also be thicker or thin- ner, provided that the created layer allows nanochannels to be made and that a successful bond can be created between the substrate and the covering layer.

The nanochannel is etched into the semiconductor ma- terial and possibly also partly in the underlying substrate.

This may be achieved by the usual etching techniques. The di- mensions of the channel depend, among other things, on the technique used. With the usual lithographic techniques a channel width from approximately 0.5 pm can be achieved. If narrower channels are desired, it is possible to use, for ex- ample, beam lithography with which even channel widths of a few tens of nanometers can be achieved. The depth of the channel is determined by the length of time during which etching takes place and can therefor be adjusted as desired.

Finally, the covering layer is bonded with the sub- strate via the layer of semiconductor material provided thereon. This occurs preferably by anodic bonding. Anodic bonding occurs by heating the assembly to a temperature of at least 350 C and preferably approximately 400°C, and by subse-

quently applying a high voltage of preferably approximately 1000 V to 1500 V to the assembly.

The invention is also embodied in nanochannels ob- tained by the above-elucidated method. These nanochannels are bounded by a substrate and a covering layer that is attached to the substrate, and are characterised by a layer of semi- conductor material bonding the substrate with the covering layer, and in which semiconductor material dopant is applied locally to form electrodes.

Hereinbelow, a few exemplary embodiments are given to elucidate the present invention.

Example 1 In this example, a preferred method for forming a nanochannel between two glass plates is given.

As substrate and covering layer glass plates of the Borofloat-type were used,. available from Bullen Ultrasonics Inc. , U. S. A. These plates were provided with pre-drilled holes as in-and outlet for the nanochannels. With the aid of LPCVD (Low Pressure Chemical Vapour Deposition) an intermedi- ate layer of amorphous silicon was applied on the substrate, having a thickness of 33 nm. With the aid of a photoresist mask the pattern of the nanochannel was applied on the inter- mediate layer, whereafter in an Alcatel fluoride etcher, the channels were etched into the intermediate layer and partly into the substrate.

Hereafter both the treated substrate with intermedi- ate layer and the covering layer were cleaned in a solution of nitric acid. Subsequently the covering layer was applied on the substrate provided with the intermediate layer and the assembly was bonded in an Electronic Visions EVG501 bonder.

To this end the assembly was preheated for two hours to 400°C, after which bonding took place at the same tempera- ture, and by applying 1000 V for one hour. In this way a na- nochannel was created having a depth of 50 nm, a width of 40 um and a length of 3 mm.

Example 2 In accordance with the method of Example 1, nano- channels of various sizes were fabricated. In one series of experiments, the channels had a depth of 50 nm and a length of 3 mm and various widths. The narrowest channel had a width of 2 um, the widest channel had a width of 100 pm. In another series of experiments, ladder-shaped channels were formed, wherein the one leg had a width of 2 um and the other leg a width of 5 um. Here also the depth of the channels was 50 nm.

The quality of the formed channels was checked with the aid of electron microscopy and fluorescence microscopy.

For the fluorescence microscopic check a fluorescent liquid (Rhodamine 6G) was fed through the formed nanochannel. In all cases the fluorescent liquid flowed through the nanochannels as a result of capillary forces, without the application of over-or underpressure. The electron microscopic image from the electron microscopic check showed no irregularities in the channel. Moreover, no leakages were observed in any of the nanochannels-fabricated in accordance with the present method.

This example shows that by the method in accordance with the present invention, nanochannels of various predeter- mined dimensions can be fabricated, without any obstructions, and through which therefore flow can take place. The nano- channels fabricated by the method according to the present invention appeared to be leakage-free.