Title:
METHOD OF MEASURING HETEROGENOUS NCULEAR NIBONUCLEOPROTEIN B1 (hnRNP B1) mRNA
Document Type and Number:
WIPO Patent Application WO/2006/011667
Kind Code:
A1
Abstract:
A method of measuring heterogeneous nuclear ribonucleoprotein B1 (hnRNP B1) mRNA contained in a sample comprising: the step of using a first primer, which is homologous with at least a part of the 5’-terminal downstream of a specific base sequence of the RNA, and a second primer, which is complementary to at least a part of the 3’-terminal upstream of the specific base sequence as described above, (wherein at least one of the first and second primers has a promoter sequence at the 5’-terminus) and thus forming a double-stranded DNA having the promoter sequence and the specific base sequence in the downstream of the promoter sequence; the step of forming an RNA transcriptional product by using the double-stranded DNA as a template; the step of forming the above-described double-stranded DNA by successively using the RNA transcriptional product as a template for the DNA synthesis; the nucleic acid amplification step of repeating the above steps under such conditions as allowing the simultaneous proceed of the individual steps; and the step of measuring the amount of the RNA transcriptional product as described above.
Inventors:
SAITO JUICHI (JP)
HAYASHI TOSHINORI (JP)
HAYASHI TOSHINORI (JP)
Application Number:
PCT/JP2005/014257
Publication Date:
February 02, 2006
Filing Date:
July 28, 2005
Export Citation:
Assignee:
TOSOH CORP (JP)
SAITO JUICHI (JP)
HAYASHI TOSHINORI (JP)
SAITO JUICHI (JP)
HAYASHI TOSHINORI (JP)
International Classes:
C12Q1/68; C12N15/09; G01N21/78
Other References:
SUEOKA E. ET AL: "Heterogeneous Nuclear Ribonucleoprotein B1 as Early Cancer Biomarker for Occult Cancer of Human Lungs and Bronchial Dysplasia.", CANCER RESEARCH., vol. 61, 2001, pages 1896 - 1902, XP002992958
BURD G. ET AL: "Primary structures of the heterogeneous nuclear ribonucleoprotein A2, B1, and C2proteins: A diversity of RNA binding proteins is generated by small peptide inserts.", PROC ACAD SCI USA., vol. 86, 1989, pages 9788 - 9792, XP002024995
ISHIGURO T. ET AL: "Intercalation activating fluorescence DNA probe and its application to homogeneous quantification of a target sequence by isothermal sequence amplification in a closed vessel.", ANALYTICAL BIOCHEMISTRY., vol. 314, 2003, pages 77 - 86, XP001165733
See also references of EP 1783232A4
BURD G. ET AL: "Primary structures of the heterogeneous nuclear ribonucleoprotein A2, B1, and C2proteins: A diversity of RNA binding proteins is generated by small peptide inserts.", PROC ACAD SCI USA., vol. 86, 1989, pages 9788 - 9792, XP002024995
ISHIGURO T. ET AL: "Intercalation activating fluorescence DNA probe and its application to homogeneous quantification of a target sequence by isothermal sequence amplification in a closed vessel.", ANALYTICAL BIOCHEMISTRY., vol. 314, 2003, pages 77 - 86, XP001165733
See also references of EP 1783232A4
Attorney, Agent or Firm:
Aoki, Atsushi (ISHIDA & ASSOCIATES Toranomon 37 Mori
Bldg., 5-1, Toranomon 3-chom, Minato-ku Tokyo 23, JP)
Download PDF: