Title:
METHOD OF MODIFYING CHROMOSOME
Document Type and Number:
WIPO Patent Application WO/2007/063919
Kind Code:
A1
Abstract:
It is intended to provide a widely usable method of modifying chromosome without
resorting to any specific enzyme or sequence and a method of identifying a gene
that is essentially required in cell proliferation. First, a selection marker
gene (B) allowing negative selection is integrated into the upstream or downstream
of a target DNA region (X) and then the selection marker gene (B) and the target
DNA region (X) are sandwiched between duplicated sequences. It is preferable
that a DNA fragment having a sequence (A’) (which is a sequence being substantially
the same as a specific sequence (A) located in the downstream of the target DNA
region) and the selection marker gene (B) as described above is constructed and
then this DNA fragment is integrated into the upstream of the target DNA region
(X). Next, homologous recombination and negative selection are conducted so
that the target DNA region (X) can be easily removed from the chromosome. In the
case where the chromosome contains no target DNA region (X) after the above-described
treatment, it can be understood that the target DNA region (X) contains no gene
that is essentially required in cell proliferation under the culture condition.
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Inventors:
HIRASHIMA KYOTARO (JP)
TOHDA HIDEKI (JP)
HAMA YUKO (JP)
TOHDA HIDEKI (JP)
HAMA YUKO (JP)
Application Number:
PCT/JP2006/323852
Publication Date:
June 07, 2007
Filing Date:
November 29, 2006
Export Citation:
Assignee:
ASAHI GLASS CO LTD (JP)
HIRASHIMA KYOTARO (JP)
TOHDA HIDEKI (JP)
HAMA YUKO (JP)
HIRASHIMA KYOTARO (JP)
TOHDA HIDEKI (JP)
HAMA YUKO (JP)
International Classes:
C12N15/00; C12N15/09; C12Q1/68
Foreign References:
| JP2003144164A | 2003-05-20 |
Other References:
LANGLE-ROUAULT F. ET AL.: "A method for performing precise alterations in the yeast genome using a recycable selectable marker", NUCLEIC ACIDS RES., vol. 23, no. 15, 1995, pages 3079 - 3081, XP003013294
HIRASHIMA K. ET AL.: "Bunretsu Kobo Senshokutai Kaihenho no Koritsuka", ANNUAL MEETING OF THE MOLECULAR BIOLOGY SOCIETY OF JAPAN PROGRAM KOEN YOSHISHU, vol. 27, 2004, pages 880 (3PA-346), XP003013295
WILSON R.B. ET AL.: "A recyclable Candida albicans URA3 cassette for PCR product-directed gene disruptions", YEAST, vol. 16, no. 1, 2000, pages 65 - 70, XP003013296
KAWAHATA M. ET AL.: "Kobo Keishitsu Tenkan ni Okeru Sentaku Marker Idenshi no Recycle", THE SOCIETY FOR BIOTECHNOLOGY, JAPAN TAIKAI KOEN YOSHISHU, vol. 1996, 1996, pages 313 (1029), XP003013297
HIRASHIMA K. ET AL.: "A simple and effective chromosome modification method for large-scale deletion of genome sequences and identification of essential genes in fission yeast", NUCLEIC ACIDS RES., vol. 34, no. 2, 24 January 2006 (2006-01-24), pages 1 - 7, XP003013298
HIRASHIMA K. ET AL.: "Latour system Senshokutai Kaihen Gijutsu o Oyo shita Aratana Hissu Idenshi Hanteiho", BIOTECHNOLOGY JOURNAL, vol. 6, no. 4, 1 July 2006 (2006-07-01), pages 458 - 461, XP003013299
GHOSH, K.; DUYNEG. D.: "Cre- loxP biochemistry", METHODS, vol. 28, 2002, pages 374 - 383
DATSENKO, K. A.; WANNER,B. L: "One-step inactivation of chromosomal genes in Escherichia coli K-12 usingPCR products", PROC. NATL. ACAD. SCI. USA, vol. 97, 2000, pages 6640 - 6645, XP002210218, DOI: doi:10.1073/pnas.120163297
POSFAI, G.; KOLISNYCHENKO, V.; BERESZKI, Z.; BLATTNER, F. R.: "Markerless gene replacement in Escherichia colistimulated by a double- strand break in the chromosome", NUCL.ACIDS RES., vol. 27, 1999, pages 4409 - 4415, XP002963851, DOI: doi:10.1093/nar/27.22.4409
LANGLE-ROUAULT, F.; JACOBS, E.: "Amethod for performing precise alterations in the yeast genome using are cyclable selectable marker", NUCL.ACIDS RES., vol. 23, 1995, pages 3079 - 3081
WILSON, R. B.; DAVIS, D.; ENLOE, B. M.; MITCHELL, A. P.: "A recyclable Candida albicans URA3 cassette for PCR product-directed gene disruptions", YEAST, vol. 16, 2000, pages 65 - 70, XP003013296, DOI: doi:10.1002/(SICI)1097-0061(20000115)16:1<65::AID-YEA508>3.0.CO;2-M
GRIMM, C.; KOHLI, J.; MURRAY, J.; MAUNDRELL, K., GENETIC ENGINEERING OF SCHIZOSACCHAROMYCES POMBE: A SYSTEM FOR GENE DISRUPTION
See also references of EP 1953224A4
HIRASHIMA K. ET AL.: "Bunretsu Kobo Senshokutai Kaihenho no Koritsuka", ANNUAL MEETING OF THE MOLECULAR BIOLOGY SOCIETY OF JAPAN PROGRAM KOEN YOSHISHU, vol. 27, 2004, pages 880 (3PA-346), XP003013295
WILSON R.B. ET AL.: "A recyclable Candida albicans URA3 cassette for PCR product-directed gene disruptions", YEAST, vol. 16, no. 1, 2000, pages 65 - 70, XP003013296
KAWAHATA M. ET AL.: "Kobo Keishitsu Tenkan ni Okeru Sentaku Marker Idenshi no Recycle", THE SOCIETY FOR BIOTECHNOLOGY, JAPAN TAIKAI KOEN YOSHISHU, vol. 1996, 1996, pages 313 (1029), XP003013297
HIRASHIMA K. ET AL.: "A simple and effective chromosome modification method for large-scale deletion of genome sequences and identification of essential genes in fission yeast", NUCLEIC ACIDS RES., vol. 34, no. 2, 24 January 2006 (2006-01-24), pages 1 - 7, XP003013298
HIRASHIMA K. ET AL.: "Latour system Senshokutai Kaihen Gijutsu o Oyo shita Aratana Hissu Idenshi Hanteiho", BIOTECHNOLOGY JOURNAL, vol. 6, no. 4, 1 July 2006 (2006-07-01), pages 458 - 461, XP003013299
GHOSH, K.; DUYNEG. D.: "Cre- loxP biochemistry", METHODS, vol. 28, 2002, pages 374 - 383
DATSENKO, K. A.; WANNER,B. L: "One-step inactivation of chromosomal genes in Escherichia coli K-12 usingPCR products", PROC. NATL. ACAD. SCI. USA, vol. 97, 2000, pages 6640 - 6645, XP002210218, DOI: doi:10.1073/pnas.120163297
POSFAI, G.; KOLISNYCHENKO, V.; BERESZKI, Z.; BLATTNER, F. R.: "Markerless gene replacement in Escherichia colistimulated by a double- strand break in the chromosome", NUCL.ACIDS RES., vol. 27, 1999, pages 4409 - 4415, XP002963851, DOI: doi:10.1093/nar/27.22.4409
LANGLE-ROUAULT, F.; JACOBS, E.: "Amethod for performing precise alterations in the yeast genome using are cyclable selectable marker", NUCL.ACIDS RES., vol. 23, 1995, pages 3079 - 3081
WILSON, R. B.; DAVIS, D.; ENLOE, B. M.; MITCHELL, A. P.: "A recyclable Candida albicans URA3 cassette for PCR product-directed gene disruptions", YEAST, vol. 16, 2000, pages 65 - 70, XP003013296, DOI: doi:10.1002/(SICI)1097-0061(20000115)16:1<65::AID-YEA508>3.0.CO;2-M
GRIMM, C.; KOHLI, J.; MURRAY, J.; MAUNDRELL, K., GENETIC ENGINEERING OF SCHIZOSACCHAROMYCES POMBE: A SYSTEM FOR GENE DISRUPTION
See also references of EP 1953224A4
Attorney, Agent or Firm:
SENMYO, Kenji et al. (38 Kanda-Higashimatsushitacho, Chiyoda-k, Tokyo 42, JP)
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