Title:
METHOD FOR MODIFYING PLANT MORPHOLOGY, BIOCHEMISTRY AND PHYSIOLOGY USING CYTOKININ OXIDASES
Document Type and Number:
WIPO Patent Application WO2001096580
Kind Code:
A3
Abstract:
The present invention relates to methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism comprising expression of a plant cytokinin oxidase or comprising expression of another protein that reduces the level of active cytokinins in plants or plant parts. The invention also relates to novel plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising said sequences. The invention also relates to the use of said sequences for improving root-related characteristics including increasing yield and/or enhancing early vigor and/or modifying root/shoot ratio and/or improving resistance to lodging and/or increasing drought tolerance and/or promoting in vitro propagation of explants and/or modifying cell fate and/or plant development and/or plant morphology and/or plant biochemistry and/or plant physiology. The invention also relates to the use of said sequences in the above-mentioned methods.The invention also relates to methods for identifying and obtaining proteins and compounds interacting with cytokinin oxidase proteins. The invention also relates to the use of said compounds as a plant growth regulator or herbicide.
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Inventors:
SCHMULLING THOMAS (DE)
WERNER TOMAS (DE)
WERNER TOMAS (DE)
Application Number:
PCT/EP2001/006833
Publication Date:
July 11, 2002
Filing Date:
June 18, 2001
Export Citation:
Assignee:
SCHMULLING THOMAS (DE)
WERNER TOMAS (DE)
WERNER TOMAS (DE)
International Classes:
C07K16/40; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/02; C12N9/06; C12N15/09; C12N15/53; C12N15/82; C12Q1/26; (IPC1-7): C12N15/53; C12N15/82; C12N9/06; C12N1/21; C12N1/19; C12N5/10; A01H5/00; C12Q1/26
Domestic Patent References:
| WO1999006571A1 | 1999-02-11 |
Other References:
ROY O. MORRIS ET AL.,: "Isolation of a gene encoding a glycosylated cytokinin oxidase from maize", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 255, 1999, pages 328 - 333, XP002151605
DATABASE SWALL 1 January 1998 (1998-01-01), S.D. ROUNSLEY ET AL.,, XP002151606
DATABASE SWALL 1 May 2000 (2000-05-01), M. BEVAN ET AL.,: "Cytokinin oxidase-like protein", XP002151607
DATABASE SWALL 1 May 1999 (1999-05-01), X. LIN ET AL: "Arabidopsis thaliana chromosome II BAC F3P11 genomic sequence. Putative Cytokinin Oxidase", XP002151608
DATABASE BIOSIS BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; M. FRANK ET AL.,: "TSD genes negatively regulate merismetic activity in Arabidopsis", XP002151616
DATABASE BIOSIS BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; ZHANG, N. ET AL.,: "Initiation and elongation of lateral roots in Lactuca sativa", XP002151609
DATABASE BIOSIS BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; Y. KODA ET AL.,: "Cytokinin production by tomato root. Identification of a major cytokinin produced by the root and environmental fcators affecting the production", XP002151610
DATABASE SWALL 1 January 1998 (1998-01-01), S.D. ROUNSLEY ET AL.,, XP002151606
DATABASE SWALL 1 May 2000 (2000-05-01), M. BEVAN ET AL.,: "Cytokinin oxidase-like protein", XP002151607
DATABASE SWALL 1 May 1999 (1999-05-01), X. LIN ET AL: "Arabidopsis thaliana chromosome II BAC F3P11 genomic sequence. Putative Cytokinin Oxidase", XP002151608
DATABASE BIOSIS BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; M. FRANK ET AL.,: "TSD genes negatively regulate merismetic activity in Arabidopsis", XP002151616
DATABASE BIOSIS BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; ZHANG, N. ET AL.,: "Initiation and elongation of lateral roots in Lactuca sativa", XP002151609
DATABASE BIOSIS BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; Y. KODA ET AL.,: "Cytokinin production by tomato root. Identification of a major cytokinin produced by the root and environmental fcators affecting the production", XP002151610
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