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Title:
METHOD FOR OBTAINING EUKARYOTE CELL CAPABLE OF EXPRESSING GAS VESICLE PROTEIN COMPLEX, EUKARYOTE CELL, METHOD FOR PRODUCING GAS VESICLE PROTEIN COMPLEX USING EUKARYOTE CELL, AND KIT
Document Type and Number:
WIPO Patent Application WO/2018/043716
Kind Code:
A1
Abstract:
The present invention comprises an introduction step for introducing a gas vesicle gene gvpA and a gas vesicle gene gvpC into a eukaryote cell so as to be expressible therein, wherein the gas vesicle gvpC is derived from cyanobacteria.

Inventors:
MIZUSHIMA RYOTA (JP)
WATANABE TOMONOBU (JP)
Application Number:
PCT/JP2017/031637
Publication Date:
March 08, 2018
Filing Date:
September 01, 2017
Export Citation:
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Assignee:
RIKEN (JP)
International Classes:
C12N5/10; C07K14/195; C12N15/09; C12P21/02
Domestic Patent References:
WO1990010071A11990-09-07
Other References:
TIANHE, W. ET AL.: "Floating Escherichia coli by Expressing Cyanobacterial Gas Vesicle Genes", J. OCEAN UNIV. CHINA, vol. 14, no. l, 2015, pages 84 - 88, XP035427750
BEARD, S. J. ET AL.: "The diversity of gas vesicle genes in Planktothrix rubescens from Lake Zurich", MICROBIOLOGY, vol. 145, no. 10, 1999, pages 2757 - 2768, XP055470800
BEARD, S. J. ET AL.: "Gas vesicle genes in Planktothrix rubescens from Nordic lakes: strains with weak gas vesicles possess a longer variant of gvpC", MICROBIOLOGY, vol. 146, no. 18, 2000, pages 2009 - 2018
"OptimumGENETM Codon Saitekika", GENSCRIPT, 1 August 2016 (2016-08-01), Retrieved from the Internet [retrieved on 20171110]
"Transposon ni yori Genome eno Kumikomi", JOKYO GA KANO NA HATSUGEN SYSTEM, 10 August 2016 (2016-08-10), Retrieved from the Internet [retrieved on 20171110]
Attorney, Agent or Firm:
HARAKENZO WORLD PATENT & TRADEMARK (JP)
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