Title:
METHOD FOR OVEREXPRESSING TARGET GENES USING ELECTROMAGNETIC-RESPONSIVE PROMOTER
Document Type and Number:
WIPO Patent Application WO/2020/080920
Kind Code:
A3
Abstract:
The present invention pertains to a method for overexpressing target genes and/or reprogramming cells, the method comprising: (a) obtaining transformed cells by introducing a vector into which a promoter and target genes have been inserted; and (b) applying electromagnetic waves to the transformed cells obtained in step (a), and to a method for treating a disease using the methods. When the method for overexpressing target genes by using an electromagnetic-responsive promoter is used, the amount of expression of desired target genes can be simply and artificially regulated inside and outside cells, and the expression of the target genes can be regulated until a desired specific time.
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Inventors:
KIM JONGPIL (KR)
YOO JUNSANG (KR)
YOO JUNSANG (KR)
Application Number:
PCT/KR2019/017747
Publication Date:
June 11, 2020
Filing Date:
December 13, 2019
Export Citation:
Assignee:
UNIV DONGGUK IND ACAD COOP (KR)
International Classes:
C12N13/00; A61K35/30; A61K35/32; A61P25/00; C12N5/077; C12N5/0793; C12N15/67
Domestic Patent References:
| WO2012035120A2 | 2012-03-22 |
Foreign References:
| KR101518096B1 | 2015-05-07 | |||
| KR20150045557A | 2015-04-29 |
Other References:
ANTON, M.: "Optimization of radiation controlled gene expression by adenoviral vectors in vitro", CANCER GENE THERAPY, vol. 12, no. 7, 1 April 2005 (2005-04-01), pages 640 - 646, XP055717037
YOO JUNSANG, LEE EUIYEON, KIM HEE YOUNG, YOUN DONG-HO, JUNG JUNGHYUN, KIM HONGWON, CHANG YUJUNG, LEE WONWOONG, SHIN JAEIN, BAEK SO: "Electromagnetized gold nanoparticles mediate direct lineage reprogramming into induced dopamine neurons in vivo for Parkinson's disease therapy", NATURE NANOTECHNOLOGY, 17 July 2017 (2017-07-17), pages 1006 - 1014, XP055717043
SEONG, Y.: "Egrl mediated the neuronal differentiation induced by extremely low-frequency electromagnetic fields", LIFE SCIENCES, 3 March 2014 (2014-03-03), pages 16 - 27, XP028845327
YOO JUNSANG, LEE EUIYEON, KIM HEE YOUNG, YOUN DONG-HO, JUNG JUNGHYUN, KIM HONGWON, CHANG YUJUNG, LEE WONWOONG, SHIN JAEIN, BAEK SO: "Electromagnetized gold nanoparticles mediate direct lineage reprogramming into induced dopamine neurons in vivo for Parkinson's disease therapy", NATURE NANOTECHNOLOGY, 17 July 2017 (2017-07-17), pages 1006 - 1014, XP055717043
SEONG, Y.: "Egrl mediated the neuronal differentiation induced by extremely low-frequency electromagnetic fields", LIFE SCIENCES, 3 March 2014 (2014-03-03), pages 16 - 27, XP028845327
Attorney, Agent or Firm:
SON, Min (KR)
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