Title:
METHOD FOR PREPARING ACTIVE FORM OF TNFR-FC FUSION PROTEIN
Document Type and Number:
WIPO Patent Application WO/2013/025079
Kind Code:
A1
Abstract:
The present invention relates to a method for separating and preparing a TNFR-Fc fusion protein using hydrophobic interaction chromatography (HIC). More particularly, the present invention relates to a method for separating and preparing a highly pure active protein from clipped proteins due to displacement effect by adjusting the conductivity of a protein sample using a high concentration of salt solution and by adjusting a loading amount thereof, and a TNFR-Fc fusion protein prepared by the method.
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Inventors:
WON HYE SOON (KR)
SUNG BYUNG JE (KR)
AHN YONG HO (KR)
PARK SANG KYUNG (KR)
SUNG BYUNG JE (KR)
AHN YONG HO (KR)
PARK SANG KYUNG (KR)
Application Number:
PCT/KR2012/006564
Publication Date:
February 21, 2013
Filing Date:
August 17, 2012
Export Citation:
Assignee:
HANWHA CHEMICAL CORP (KR)
WON HYE SOON (KR)
SUNG BYUNG JE (KR)
AHN YONG HO (KR)
PARK SANG KYUNG (KR)
WON HYE SOON (KR)
SUNG BYUNG JE (KR)
AHN YONG HO (KR)
PARK SANG KYUNG (KR)
International Classes:
C07K1/20; C07K1/18; C07K1/22; C07K19/00
Domestic Patent References:
| WO2005075498A1 | 2005-08-18 | |||
| WO2005042569A1 | 2005-05-12 | |||
| WO2003072060A2 | 2003-09-04 |
Foreign References:
| US20050136521A1 | 2005-06-23 |
Other References:
KYOGOKU, K. ET AL.: "PRODUCTION OF RECOMBINANT TUMOR NECROSIS FACTOR RECEPTOR/FC FUSION PROTEIN BY GENETICALLY MANIPULATED CHICKEN", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 105, no. 5, 2008, pages 454 - 459, XP022733110
EVANS, D. R. H. ET AL.: "Purification of an Fc-fusion biologic: Clearance of multiple product related impurities by hydrophobic interaction chromatography", JOURNAL OF CHROMATOGRAPHY A, vol. 1177, 2008, pages 265 - 271, XP022401694
TIWARI, N. ET AL.: "Identification and characterization of native proteins of Escherichia coli BL-21 that display affinity towards Immobilized Metal Affinity Chromatography and Hydrophobic Interaction Chromatography Matrices", PROTEIN EXPRESSIO AND PURIFICATION, vol. 70, 2010, pages 191 - 195, XP026915652
ZOLODZ, M. D. ET AL.: "Separation by hydrophobic interaction chromatography and structural determination by mass spectrometry of mannosylated glycoforms of a recombinant transferrin-exendin-4 fusion protein from yeast", JOURNAL OF CHROMATOGRAPHY A, vol. 1217, no. 2, 2010, pages 225 - 234, XP026817105
KOHNO, T. ET AL.: "Binding Characteristics of Tumor Necrosis Factor Receptor-Fc Fusion Proteins vs Anti-Tumor Necrosis Factor mAbs", J. INVESTIG. DERMATOL. SYMP PROC., vol. 12, no. 1, May 2007 (2007-05-01), pages 5 - 8, XP055144570
MURRAY, K. M. ET AL.: "RECOMBINANT HUMAN TUMOR NECROSIS FACTOR RECEPTOR (P75) FC FUSION PROTEIN (TNFR:FC) OM RHEUMATOID ARTHRITIS", THE ANNALS OF PHARMACOTHERAPY, vol. 31, no. 11, 1997, pages 1335 - 1338, XP000952552
"Hydrophobic Interaction and Reversed Phase Chromatography - Principles and Methods", 1 February 2006
EVANS, D. R. H. ET AL.: "Purification of an Fc-fusion biologic: Clearance of multiple product related impurities by hydrophobic interaction chromatography", JOURNAL OF CHROMATOGRAPHY A, vol. 1177, 2008, pages 265 - 271, XP022401694
TIWARI, N. ET AL.: "Identification and characterization of native proteins of Escherichia coli BL-21 that display affinity towards Immobilized Metal Affinity Chromatography and Hydrophobic Interaction Chromatography Matrices", PROTEIN EXPRESSIO AND PURIFICATION, vol. 70, 2010, pages 191 - 195, XP026915652
ZOLODZ, M. D. ET AL.: "Separation by hydrophobic interaction chromatography and structural determination by mass spectrometry of mannosylated glycoforms of a recombinant transferrin-exendin-4 fusion protein from yeast", JOURNAL OF CHROMATOGRAPHY A, vol. 1217, no. 2, 2010, pages 225 - 234, XP026817105
KOHNO, T. ET AL.: "Binding Characteristics of Tumor Necrosis Factor Receptor-Fc Fusion Proteins vs Anti-Tumor Necrosis Factor mAbs", J. INVESTIG. DERMATOL. SYMP PROC., vol. 12, no. 1, May 2007 (2007-05-01), pages 5 - 8, XP055144570
MURRAY, K. M. ET AL.: "RECOMBINANT HUMAN TUMOR NECROSIS FACTOR RECEPTOR (P75) FC FUSION PROTEIN (TNFR:FC) OM RHEUMATOID ARTHRITIS", THE ANNALS OF PHARMACOTHERAPY, vol. 31, no. 11, 1997, pages 1335 - 1338, XP000952552
"Hydrophobic Interaction and Reversed Phase Chromatography - Principles and Methods", 1 February 2006
Attorney, Agent or Firm:
SON, Min (6th Floor, 36, Teheranro 87 gil, Gangnam-gu, Seoul 135-973, KR)
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