METHOD FOR PREPARING FLUORESCENT SILK PROTEIN SOLUTION EXTRACTED FROM TRANSGENIC COCOONS AND METHOD FOR MANUFACTURING SCAFFOLD USING SAME

WIPO Patent Application WO/2017/030277

A1

The present invention provides a method for preparing a fluorescent silk fibroin solution, the method comprising the steps of: a) putting fluorescent cocoons in an aqueous solution containing a refining agent, followed by heating at 40-60℃ for 8-24 h and then washing with distilled water, thereby obtaining refined fluorescent silk fibroin; b) dissolving the refined fluorescent silk fibroin in a solvent, in which 15 mg to 1.5 g of dithiothreitol (DTT) is mixed with each 100 ㎖ of 9-9.6 M LiBr, at 40-60℃ for 1-5 h; and c) dialyzing the dissolved fluorescent silk fibroin in primary distilled water for 48-96 h. The refining agent preferably contains alcalase and sodium bicarbonate (NaHCO3). An existing method for preparing a silk fibroin solution from a fluorescent cocoon has disadvantages in that silk fibroin is not dissolved at a low temperature and the fluorescence is lost. However, according to the present invention, a method for adding a reductant has advantages in that a fluorescent silk fibroin solution can be prepared at a low temperature and the fluorescence is maintained. Therefore, according to the present invention, the fluorescent silk fibroin can be mass-produced and a biocompatible fluorescent protein can be easily prepared and provided, and thus, materials can be provided that are applicable to bio-industries, such as scaffolds for tissue regeneration, bio-imaging, and biosensors through and biochips.

PARK CHAN HUM (KR)
KIM SOO HYEON (KR)
KIM DONG WOOK (KR)
MOON BO MI (KR)
PARK HYUN JUNG (KR)
PARK YE RI (KR)
LEE MIN CHAE (KR)
LEE OK JOO (KR)
LEE JUNG MIN (KR)
JEONG JU YEON (KR)
JU HYUNG WOO (KR)

PCT/KR2016/006149

February 23, 2017

June 10, 2016

Click for automatic bibliography generation  

UNIV HALLYM IND ACAD COOP FOUND (KR)

A61K49/00; C07K14/435; A61K49/04; A61K49/14; C07K1/14

MYUNG ET AL.: "Fluorescent Silk Fibroin Nanoparticles Prepared Using a Reverse Microemulsion", MACROMOLECULAR RESEARCH, V, vol. 16, no. 7, 2008, pages 604 - 608, XP055245648
PARK, YEONG HWAN ET AL.: "Development and Utilization of Silk Protein Scaffold for Tissue Engineering", RESEARCH REPORT(RUML DEVELOPMENT ADMINISTRATION, 2008, Seoul National University, College of Agricultrue and Life Sciences, pages 1 - 111
IIZUKA ET AL.: "Colored Fluorescent Silk Made by Transgenic Silkworms", ADVANCED FUNCTIONAL MATERIALS, vol. 23, 2013, pages 5232 - 5239, XP055088490
PARK, YEONG HWAN ET AL.: "Development and Utilization of Silk Protein Scaffold for Tissue Engineering(Third-year", THE 6TH OSONG INTERNATIONAL BIO SYMPOSIUM/ 2ND SYMPOSIUM OF THE KOREAN ASSOCIATION OF SOCIETIES FOR PLANT SCIENCE, 1 November 2007 (2007-11-01), Ramada Plaza Cheongju Hotel, pages 425 - 426
KIM, HYE LIN ET AL.: "Preparation and Chatactenzatioti of Silk Fibroin/Gelatin Hybrid Scaffolds", POLYMER (KOREA), vol. 35, no. 5, 2011, pages 378 - 384

TNI IP LAW FIRM (KR)

View/Download PDF      PDF Help

Previous Patent: MEDICAL IMAGE DISPLAY DEVICE AND MEDICAL IMAGE PROCESSING METHOD

Next Patent: LED DEVICE MANUFACTURING METHOD AND LED DEVICE MANUFACTURING APPARATUS USING PHOSPHORESCENT LIQUID S...