Title:
METHOD FOR PREPARING L-2-AMINOBUTYRIC ACID BY MEANS OF DOUBLE ENZYME SERIES CONNECTION
Document Type and Number:
WIPO Patent Application WO/2021/143356
Kind Code:
A1
Abstract:
Disclosed is a method for preparing L-2-aminobutyric acid by means of double enzyme series connection, relating to the field of bioengineering. By respectively culturing recombinant Escherichia coli expressing L-glutamate mutase and L-aspartate-β-decarboxylase, L-glutamate mutase and L-aspartate-β-decarboxylase are obtained. Two enzymes are added to a reaction system at a certain mass ratio, and L-glutamic acid is used as a substrate to carry out an enzyme reaction to prepare L-2-aminobutyric acid. When the amount of L-aspartate-β-decarboxylase is 2 mg/mL, the reaction is carried out for 24 h, and conversion is carried out to generate 8.5 mmol/L of L-2 aminobutyric acid, the molar conversion rate being 85.00%. Compared with chemical production methods, the method has a safe production process and causes no environmental pollution. Compared with a multi-enzyme synthesis system using threonine as a substrate, the substrate is cheaper and the process is simpler.
Inventors:
ZHOU ZHEMIN (CN)
LIU ZHONGMEI (CN)
LIU YUFENG (CN)
ZHOU LI (CN)
CUI WENJING (CN)
GUO JUNLING (CN)
LIU ZHONGMEI (CN)
LIU YUFENG (CN)
ZHOU LI (CN)
CUI WENJING (CN)
GUO JUNLING (CN)
Application Number:
PCT/CN2020/131276
Publication Date:
July 22, 2021
Filing Date:
November 25, 2020
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N9/88; C12P13/04; C12N9/90; C12N15/60; C12N15/61; C12N15/70; C12R1/19
Foreign References:
| CN111172213A | 2020-05-19 | |||
| CN106148259A | 2016-11-23 | |||
| CN109536429A | 2019-03-29 | |||
| CN109266595A | 2019-01-25 | |||
| CN109777845A | 2019-05-21 | |||
| CN105671098A | 2016-06-15 |
Other References:
LIU YUFENG, HAN LAICHUANG, CHENG ZHONGYI, LIU ZHONGMEI, ZHOU ZHEMIN: "-Glutamate as the Sole Substrate", ACS CATALYSIS, AMERICAN CHEMICAL SOCIETY, US, vol. 10, no. 23, 4 December 2020 (2020-12-04), US, pages 13913 - 13917, XP055829641, ISSN: 2155-5435, DOI: 10.1021/acscatal.0c04141
CHEN HAO-PING, MARSH E. NEIL G.: "Adenosylcobalamin-Dependent Glutamate Mutase: Examination of Substrate and Coenzyme Binding in an Engineered Fusion Protein Possessing Simplified Subunit Structure and Kinetic Properties †", BIOCHEMISTRY, vol. 36, no. 48, 1 December 1997 (1997-12-01), pages 14939 - 14945, XP055829642, ISSN: 0006-2960, DOI: 10.1021/bi971374g
PAN GUOSHENG ，, RENCHAO ZHENG, YUGUO ZHENG: "Advance in the biosynthesis of L-2-aminobutyrate", BULLETIN OF FERMENTATION SCIENCE AND TECHNOLOGY, ZHEJIANG UNIVERSITY OF TECHNOLOGY, CN, vol. 45, no. 3, 1 August 2016 (2016-08-01), CN, pages 182 - 187, XP055829673, ISSN: 1674-2214, DOI: 10.16774/j.cnki.issn.1674-2214.2016.03.011
LIU SHAN, LI YUAN;ZHU JUN: "One-pot Enzymatic Synthesis of L-2-Aminobutyric Acid Coupling with a NADH Regeneration System Based on Ketoreductase", CHINA BIOTECHNOLOGY, vol. 37, no. 1, 1 January 2017 (2017-01-01), pages 64 - 70, XP055829672, DOI: 10.13523/j.cb.20170110
CHEN HAO-PING, MARSH E. NEIL G.: "Adenosylcobalamin-Dependent Glutamate Mutase: Examination of Substrate and Coenzyme Binding in an Engineered Fusion Protein Possessing Simplified Subunit Structure and Kinetic Properties †", BIOCHEMISTRY, vol. 36, no. 48, 1 December 1997 (1997-12-01), pages 14939 - 14945, XP055829642, ISSN: 0006-2960, DOI: 10.1021/bi971374g
PAN GUOSHENG ，, RENCHAO ZHENG, YUGUO ZHENG: "Advance in the biosynthesis of L-2-aminobutyrate", BULLETIN OF FERMENTATION SCIENCE AND TECHNOLOGY, ZHEJIANG UNIVERSITY OF TECHNOLOGY, CN, vol. 45, no. 3, 1 August 2016 (2016-08-01), CN, pages 182 - 187, XP055829673, ISSN: 1674-2214, DOI: 10.16774/j.cnki.issn.1674-2214.2016.03.011
LIU SHAN, LI YUAN;ZHU JUN: "One-pot Enzymatic Synthesis of L-2-Aminobutyric Acid Coupling with a NADH Regeneration System Based on Ketoreductase", CHINA BIOTECHNOLOGY, vol. 37, no. 1, 1 January 2017 (2017-01-01), pages 64 - 70, XP055829672, DOI: 10.13523/j.cb.20170110
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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