Title:
METHOD FOR PREPARING N-ACETYLGALACTOSAMINE TRANSFERASE
Document Type and Number:
WIPO Patent Application WO/2021/115011
Kind Code:
A1
Abstract:
Disclosed is a prokaryotic expression system for expressing N-acetylgalactosamine transferase and a prokaryotic expression vector for expressing N-acetylgalactosamine transferase. The prokaryotic expression vector comprises a ppGalNAc-T protein expression cassette and a PDI protein expression cassette. The prokaryotic expression vector and the prokaryotic expression system for expressing N-acetylgalactosamine transferase use a co-expression single plasmid as well as a host cell having an intracellular oxidizing environment and perform the expression via a conventional general culture medium. Follow-up operations such as refolding are not required in the prokaryotic expression system and an operation method thereof.
Inventors:
ZHANG YAN (CN)
LIANG TAO (CN)
XU ZHIJUE (CN)
JIA WENJUAN (CN)
LIANG TAO (CN)
XU ZHIJUE (CN)
JIA WENJUAN (CN)
Application Number:
PCT/CN2020/128291
Publication Date:
June 17, 2021
Filing Date:
November 12, 2020
Export Citation:
Assignee:
UNIV SHANGHAI JIAOTONG (CN)
International Classes:
C12N9/10; C12N15/70
Domestic Patent References:
| WO2011051733A2 | 2011-05-05 |
Foreign References:
| CN111057688A | 2020-04-24 | |||
| CN1483077A | 2004-03-17 | |||
| CN106811450A | 2017-06-09 |
Other References:
JENNIFER LAUBER, RENÉ HANDRICK, SEBASTIAN LEPTIHN, PETER DÜRRE , SABINE GAISSER: "Expression of the functional recombinant human glycosyltransferase GalNAcT2 in Escherichia coli", MICROBIAL CELL FACTORIES, vol. 14, no. 1, 3, 31 December 2015 (2015-12-31), XP021209960, ISSN: 1475-2859, DOI: 10.1186/s12934-014-0186-0
SU PENG, GONG GUO-LI: "Research Progress on Optimizing the Expression of Exogenous Proteins in Escherichia coli", BIOTECHNOLOGY BULLETIN, vol. 33, no. 2, 8 February 2017 (2017-02-08), pages 16 - 23, XP055820194, ISSN: 1002-5464, DOI: 10.13560/j.cnki.biotech.bull.1985.2017.02.003
MA RONG , XU HAO , DING RUI , AO YONG-HUA , ZHANG LI-JUN: "The Strategy of Gene Co-Expression in Escherichia coli", CHINA BIOTECHNOLOGY, vol. 32, no. 4, 31 December 2012 (2012-12-31), pages 117 - 122, XP055810904, ISSN: 1671-8135, DOI: 10.13523/j.cb.20120419
JIA WEI: "Prokaryotic Expression, Purification and Activity Assay of Human Polypeptide: N-acetylgalactosaminyltransferase2", BASIC SCIENCES, CHINA MASTER’S THESES FULL-TEXT DATABASE, no. 05, 15 May 2006 (2006-05-15), XP009528433, ISSN: 1674-0246
SU PENG, GONG GUO-LI: "Research Progress on Optimizing the Expression of Exogenous Proteins in Escherichia coli", BIOTECHNOLOGY BULLETIN, vol. 33, no. 2, 8 February 2017 (2017-02-08), pages 16 - 23, XP055820194, ISSN: 1002-5464, DOI: 10.13560/j.cnki.biotech.bull.1985.2017.02.003
MA RONG , XU HAO , DING RUI , AO YONG-HUA , ZHANG LI-JUN: "The Strategy of Gene Co-Expression in Escherichia coli", CHINA BIOTECHNOLOGY, vol. 32, no. 4, 31 December 2012 (2012-12-31), pages 117 - 122, XP055810904, ISSN: 1671-8135, DOI: 10.13523/j.cb.20120419
JIA WEI: "Prokaryotic Expression, Purification and Activity Assay of Human Polypeptide: N-acetylgalactosaminyltransferase2", BASIC SCIENCES, CHINA MASTER’S THESES FULL-TEXT DATABASE, no. 05, 15 May 2006 (2006-05-15), XP009528433, ISSN: 1674-0246
Attorney, Agent or Firm:
J.Z.M.C. PATENT AND TRADEMARK LAW OFFICE (GENERAL PARTNERSHIP) (CN)
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