Title:
METHOD OF PREPARING PROBE DNA FOR NUCLEIC ACID CODING FOR MEMBRANE PROTEIN, METHOD OF DETECTING EXPRESSION OF GENE CODING FOR MEMBRANE PROTEIN, AND CARRIER FOR PROBE DNA IMMOBILIZATION
Document Type and Number:
WIPO Patent Application WO/2006/085476
Kind Code:
A1
Abstract:
A method of promptly and exhaustively detecting any expression of a gene coding for membrane protein. There is provided a method of preparing a probe DNA for nucleic acid coding for membrane protein, comprising selecting a partial domain of gene coding for membrane protein that has a base sequence, ≥ 5% of which consists of an exon sequence coding for a region essential for membrane bonding with respect to the membrane protein, and that contains cytosine and guanine in a total content of 40 to 60% and preparing a DNA composed of the base sequence of the partial domain or a base sequence complementary to the partial domain.
Inventors:
OKAMURA HIROSHI (JP)
HIRAYAMA KOUICHI (JP)
SENO MASAHARU (JP)
HIRAYAMA KOUICHI (JP)
SENO MASAHARU (JP)
Application Number:
PCT/JP2006/301805
Publication Date:
August 17, 2006
Filing Date:
February 02, 2006
Export Citation:
Assignee:
TOYO KOHAN CO LTD (JP)
UNIV OKAYAMA (JP)
OKAMURA HIROSHI (JP)
HIRAYAMA KOUICHI (JP)
SENO MASAHARU (JP)
UNIV OKAYAMA (JP)
OKAMURA HIROSHI (JP)
HIRAYAMA KOUICHI (JP)
SENO MASAHARU (JP)
International Classes:
C12N15/00; C12M1/00; C12Q1/68; G01N33/53; G01N33/551; G01N33/566
Foreign References:
| JP2005224189A | 2005-08-25 |
Other References:
KOHEN R. ET AL.: "Cloning of the mouse 5-HT6 serotonin receptor and mutagenesis studies of the third cytoplasmic loop", MOLECULAR BRAIN RESEARCH, vol. 90, no. 2, 2001, pages 110 - 117, XP002323793
MANSOUR A. ET AL.: "Delta opioid receptor mRNA distribution in the brain: Comparison to delta receptor binding and proenkephalin mRNA", JOURNAL OF CHEMICAL NEUROANATOMY, vol. 6, no. 6, 1993, pages 351 - 362, XP002987989
MONTROSE-RAFIZADEH C. ET AL.: "Cellular differentiation regulates expression of Cl- transport and cystic fibrosis transmembrane conductance regulator mRNA in human intestinal cells", J. BIOL. CHEM., vol. 266, no. 7, 1991, pages 4495 - 4499, XP002987990
SMUTZER G. ET AL.: "Inositol 1,4,5-trisphosphate receptor expression in odontoblast cells", BIOCHIMICA ET BIOPHYSICA ACTA (BBA) - MOLECULAR CELL RESEARCH, vol. 1358, no. 3, 1997, pages 221 - 228, XP004277697
SAMBROOK J. ET AL.: "Molecular cloning: A laboratory manual", vol. 2ND ED., 1989, COLD SPRING HABOR LABORATORY PRESS, pages: 11.12 - 11.13, XP003000715
TAKUA ET AL.: "Saibo Hyomen Marker Microarray o Mochiirua BALB/c 3T3 Saibo to Sono SV40 Keishitsu Tenkan Saibo SV-T2 no Hikaku", 27TH ANNUAL MEETING OF THE MOLECULAR BIOLOGY SOCIETY OF JAPAN PROGRAM. KOEN YOSHISHU, 25 November 2004 (2004-11-25), pages 896, 3PA-439, XP003000716
HIRAYAMA K. ET AL.: "Mouse Saibo Hyomen Marker Array o Mochiita Zokibetsu Idenshi Hatsugen no Kaiseki", 28TH ANNUAL MEETING OF THE MOLECULAR BIOLOGY SOCIETY OF JAPAN KOEN YOSHISHU, 25 November 2005 (2005-11-25), pages 746, 3P-1088, XP003000717
MANSOUR A. ET AL.: "Delta opioid receptor mRNA distribution in the brain: Comparison to delta receptor binding and proenkephalin mRNA", JOURNAL OF CHEMICAL NEUROANATOMY, vol. 6, no. 6, 1993, pages 351 - 362, XP002987989
MONTROSE-RAFIZADEH C. ET AL.: "Cellular differentiation regulates expression of Cl- transport and cystic fibrosis transmembrane conductance regulator mRNA in human intestinal cells", J. BIOL. CHEM., vol. 266, no. 7, 1991, pages 4495 - 4499, XP002987990
SMUTZER G. ET AL.: "Inositol 1,4,5-trisphosphate receptor expression in odontoblast cells", BIOCHIMICA ET BIOPHYSICA ACTA (BBA) - MOLECULAR CELL RESEARCH, vol. 1358, no. 3, 1997, pages 221 - 228, XP004277697
SAMBROOK J. ET AL.: "Molecular cloning: A laboratory manual", vol. 2ND ED., 1989, COLD SPRING HABOR LABORATORY PRESS, pages: 11.12 - 11.13, XP003000715
TAKUA ET AL.: "Saibo Hyomen Marker Microarray o Mochiirua BALB/c 3T3 Saibo to Sono SV40 Keishitsu Tenkan Saibo SV-T2 no Hikaku", 27TH ANNUAL MEETING OF THE MOLECULAR BIOLOGY SOCIETY OF JAPAN PROGRAM. KOEN YOSHISHU, 25 November 2004 (2004-11-25), pages 896, 3PA-439, XP003000716
HIRAYAMA K. ET AL.: "Mouse Saibo Hyomen Marker Array o Mochiita Zokibetsu Idenshi Hatsugen no Kaiseki", 28TH ANNUAL MEETING OF THE MOLECULAR BIOLOGY SOCIETY OF JAPAN KOEN YOSHISHU, 25 November 2005 (2005-11-25), pages 746, 3P-1088, XP003000717
Attorney, Agent or Firm:
Ohta, Akio c/o Ohta, Patent Office (New State Manor 356 23-1, Yoyogi 2-chom, Shibuya-ku Tokyo, JP)
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