Title:
METHOD FOR PREPARING TUMOR ANTIGEN-RESPONSIVE LIVING CELLULAR NANO BACKPACKS AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/104137
Kind Code:
A1
Abstract:
Disclosed are a method for preparing tumor antigen-responsive living cellular nano backpacks and the use thereof. The loading of immune lymphocyte to reduction-responsive albumin nano backpacks is achieved by means of bio-orthogonal coupling, and antigen-responsive controlled-release living cellular nano backpacks (LCNBs) are obtained. The immune lymphocyte is stimulated by a tumor antigen, which leads to an increase in the reduction potential level of the cell surface, thereby triggering the fixed-point reduction-responsive release of albumin nano backpacks. The released nano backpacks can activate immune lymphocyte in situ at a tumor lesion, feedback enhance the anti-tumor effect of immune lymphocyte, achieve efficient and synergistic tumor immunotherapy, and enhance the safety and bioavailability of the free drug in vivo.
Inventors:
CAI LINTAO (CN)
PAN HONG (CN)
ZHENG MINGBIN (CN)
LUO YINGMEI (CN)
CHEN ZE (CN)
ZHANG BAOZHEN (CN)
PAN HONG (CN)
ZHENG MINGBIN (CN)
LUO YINGMEI (CN)
CHEN ZE (CN)
ZHANG BAOZHEN (CN)
Application Number:
PCT/CN2022/137470
Publication Date:
June 15, 2023
Filing Date:
December 08, 2022
Export Citation:
Assignee:
SHENZHEN INST ADV TECH (CN)
International Classes:
A61K39/00; A61K45/06; A61K47/69; A61P35/00; C12N5/0783
Foreign References:
| CN109157662A | 2019-01-08 | |||
| CN110205291A | 2019-09-06 | |||
| CN111235106A | 2020-06-05 | |||
| CN113350283A | 2021-09-07 |
Other References:
MAXIME AYER, HARM-ANTON KLOK: "Cell-mediated delivery of synthetic nano- and microparticles", JOURNAL OF CONTROLLED RELEASE, ELSEVIER, AMSTERDAM, NL, vol. 259, 1 August 2017 (2017-08-01), AMSTERDAM, NL , pages 92 - 104, XP055754344, ISSN: 0168-3659, DOI: 10.1016/j.jconrel.2017.01.048
CHIU HSIEN-TING, SU CHENG-KUAN, SUN YUH-CHANG, CHIANG CHI-SHIUN, HUANG YU-FEN: "Albumin-Gold Nanorod Nanoplatform for Cell-Mediated Tumoritropic Delivery with Homogenous ChemoDrug Distribution and Enhanced Retention Ability", THERANOSTICS, IVYSPRING INTERNATIONAL PUBLISHER, AU, vol. 7, no. 12, 1 January 2017 (2017-01-01), AU , pages 3034 - 3052, XP093071179, ISSN: 1838-7640, DOI: 10.7150/thno.19279
YAMAN SERKAN, RAMACHANDRAMOORTHY HARISH, OTER GIZEM, ZHUKOVA DARIA, NGUYEN TAM, SABNANI MANOJ K., WEIDANZ JON A., NGUYEN KYTAI T.: "Melanoma Peptide MHC Specific TCR Expressing T-Cell Membrane Camouflaged PLGA Nanoparticles for Treatment of Melanoma Skin Cancer", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, vol. 8, 11 August 2020 (2020-08-11), pages 943, XP093004629, DOI: 10.3389/fbioe.2020.00943
ZHANG TINGRUI; TAI ZONGGUANG; CUI ZHEN; CHAI RONGRONG; ZHU QUANGANG; CHEN ZHONGJIAN: "Nano-engineered immune cells as “guided missiles” for cancer therapy", JOURNAL OF CONTROLLED RELEASE, ELSEVIER, AMSTERDAM, NL, vol. 341, 14 November 2021 (2021-11-14), AMSTERDAM, NL , pages 60 - 79, XP086930972, ISSN: 0168-3659, DOI: 10.1016/j.jconrel.2021.11.016
CHIU HSIEN-TING, SU CHENG-KUAN, SUN YUH-CHANG, CHIANG CHI-SHIUN, HUANG YU-FEN: "Albumin-Gold Nanorod Nanoplatform for Cell-Mediated Tumoritropic Delivery with Homogenous ChemoDrug Distribution and Enhanced Retention Ability", THERANOSTICS, IVYSPRING INTERNATIONAL PUBLISHER, AU, vol. 7, no. 12, 1 January 2017 (2017-01-01), AU , pages 3034 - 3052, XP093071179, ISSN: 1838-7640, DOI: 10.7150/thno.19279
YAMAN SERKAN, RAMACHANDRAMOORTHY HARISH, OTER GIZEM, ZHUKOVA DARIA, NGUYEN TAM, SABNANI MANOJ K., WEIDANZ JON A., NGUYEN KYTAI T.: "Melanoma Peptide MHC Specific TCR Expressing T-Cell Membrane Camouflaged PLGA Nanoparticles for Treatment of Melanoma Skin Cancer", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, vol. 8, 11 August 2020 (2020-08-11), pages 943, XP093004629, DOI: 10.3389/fbioe.2020.00943
ZHANG TINGRUI; TAI ZONGGUANG; CUI ZHEN; CHAI RONGRONG; ZHU QUANGANG; CHEN ZHONGJIAN: "Nano-engineered immune cells as “guided missiles” for cancer therapy", JOURNAL OF CONTROLLED RELEASE, ELSEVIER, AMSTERDAM, NL, vol. 341, 14 November 2021 (2021-11-14), AMSTERDAM, NL , pages 60 - 79, XP086930972, ISSN: 0168-3659, DOI: 10.1016/j.jconrel.2021.11.016
Attorney, Agent or Firm:
BEIJING ZHONG XUN TONG DA INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
Download PDF: