Title:
METHYLATION MARKER IN DIAGNOSIS OF BENIGN AND MALIGNANT NODULES OF THYROID CANCER AND APPLICATIONS THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/104136
Kind Code:
A1
Abstract:
The present invention relates to a methylation marker in diagnosis of benign and malignant nodules of thyroid cancer and applications thereof, in particular, to an application of a reagent for detecting the methylation state or level of at least one CpG dinucleotide of one or more target markers in preparation of a detection reagent or diagnostic kit for diagnosing benign and malignant thyroid nodules in individuals, and an application of a device for detecting the methylation state or level of at least one CpG dinucleotide of one or more target markers in preparation of a diagnostic kit for diagnosing benign and malignant thyroid nodules in individuals, the target markers comprising a PRDM16 sequence of a PRDM16 gene or genome, a CAMK2N1 sequence of a CAMK2N1 gene or genome, a TACSTD2 sequence of a TACSTD2 gene or genome, and the like. The present invention further relates to a diagnostic reagent or diagnostic kit for detecting the methylation state or methylation level of at least one CpG dinucleotide in a target marker to diagnose benign and malignant thyroid nodules.
Inventors:
SU ZHIXI (CN)
LIU YIYING (CN)
XU MINJIE (CN)
MA CHENGCHENG (CN)
LIU RUI (CN)
LIU YIYING (CN)
XU MINJIE (CN)
MA CHENGCHENG (CN)
LIU RUI (CN)
Application Number:
PCT/CN2022/137459
Publication Date:
June 15, 2023
Filing Date:
December 08, 2022
Export Citation:
Assignee:
SINGLERA GENOMICS CHINA LTD (CN)
International Classes:
C12Q1/6886; C12Q1/6883; G16B25/10
Domestic Patent References:
| WO2021143709A1 | 2021-07-22 |
Foreign References:
| CN111197087A | 2020-05-26 | |||
| CN113186278A | 2021-07-30 | |||
| US20090075265A1 | 2009-03-19 | |||
| US20180051343A1 | 2018-02-22 |
Other References:
BARROS-FILHO MATEUS CAMARGO, DOS REIS MARIANA BISARRO, BELTRAMI CAROLINE MORAES, DE MELLO JULIA BETTE HOMEM, MARCHI FÁBIO ALBUQUER: "DNA Methylation-Based Method to Differentiate Malignant from Benign Thyroid Lesions", THYROID., MARY ANN LIEBERT, NEW YORK, NY., US, vol. 29, no. 9, 1 September 2019 (2019-09-01), US , pages 1244 - 1254, XP093070690, ISSN: 1050-7256, DOI: 10.1089/thy.2018.0458
STEPHEN J. K., CHEN K. M., MERRITT J., CHITALE D., DIVINE G., WORSHAM M. J.: "Methylation markers differentiate thyroid cancer from benign nodules", JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION, vol. 41, no. 2, 1 February 2018 (2018-02-01), pages 163 - 170, XP093070693, DOI: 10.1007/s40618-017-0702-2
STEPHEN J. K., CHEN K. M., MERRITT J., CHITALE D., DIVINE G., WORSHAM M. J.: "Methylation markers differentiate thyroid cancer from benign nodules", JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION, vol. 41, no. 2, 1 February 2018 (2018-02-01), pages 163 - 170, XP093070693, DOI: 10.1007/s40618-017-0702-2
Attorney, Agent or Firm:
SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC (CN)
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