Title:
METHOD FOR PRODUCING 13-HYDROXY-9(Z)-OCTADECENOIC ACID
Document Type and Number:
WIPO Patent Application WO/2019/194287
Kind Code:
A1
Abstract:
The present invention provides a method for producing 13-hydroxy-9(Z)-octadecenoic acid, which is improved in productivity. More specifically, the present invention provides a method for producing 13-hydroxy-9(Z)-octadecenoic acid, which comprises producing 13-hydroxy-9(Z)-octadecenoic acid from linoleic acid in the presence of a transgenic microorganism capable of producing a protein selected from the group consisting of proteins (A) to (C): (A) a protein which comprises an amino acid sequence selected from the group consisting of the amino acid sequences respectively represented by SEQ ID NOs: 4, 5, 8 to 10, 13 and 14; (B) a protein which comprises an amino acid sequence that is selected from the group consisting of the amino acid sequences respectively represented by SEQ ID NOs: 4, 5, 8 to 10, 13 and 14 and has the substitution, deletion, insertion or addition of one or several amino acid residues included therein, and which has a linoleate 13-hydratase activity; and (C) a protein which comprises an amino acid sequence having identity of 90% or more with an amino acid sequence selected from the group consisting of the amino acid sequences respectively represented by SEQ ID NOs: 4, 5, 8 to 10, 13 and 14, and which has a linoleate 13-hydratase activity.
Inventors:
MIHARA YOKO (JP)
TATSUKAMI YOHEI (JP)
TAJIMA YOSHINORI (JP)
TATSUKAMI YOHEI (JP)
TAJIMA YOSHINORI (JP)
Application Number:
PCT/JP2019/015013
Publication Date:
October 10, 2019
Filing Date:
April 04, 2019
Export Citation:
Assignee:
AJINOMOTO KK (JP)
International Classes:
C12P7/42; C12P17/04; C12N9/88; C12N15/60
Domestic Patent References:
| WO2016029187A2 | 2016-02-25 | |||
| WO2008090770A1 | 2008-07-31 |
Foreign References:
| KR20150098497A | 2015-08-28 | |||
| KR20150098497A | 2015-08-28 | |||
| US20120246767A1 | 2012-09-27 | |||
| JP2002047239A | 2002-02-12 | |||
| US20040197882A1 | 2004-10-07 |
Other References:
DATABASE Protein 20 April 2014 (2014-04-20), "linoleate 13-hydratase [Lactobacillus acidophilus]", XP055645669, retrieved from NCBI Database accession no. AHW98239
DATABASE UniProt KB 20 January 2016 (2016-01-20), "SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:KRL21052.1};", XP055645679, retrieved from UniProt Database accession no. AOAOR1NM81
DATABASE Uniprot KB 25 October 2017 (2017-10-25), "SubName: Full=Oleate hydratase {ECO:0000313|EMBL:OXC20804.1};", XP055645687, Database accession no. A0A226SF70
DATABASE Uniprot KB 2 November 2016 (2016-11-02), "SubName: Full=Oleate hydratase {ECO:0000313|EMBL:OCX10702.1};", XP055645689, retrieved from UniProt Database accession no. A0A1C2D7G5
DATABASE UniProt KB 25 October 2017 (2017-10-25), "SubName: Full=Oleate hydratase {ECO:0000313|EMBL:OXC20121.1};", XP055645692, retrieved from UniProt Database accession no. A0A226SCJ9
DATABASE UniProt KB 20 January 2016 (2016-01-20), "SubName: Full=Myosin-cross-reactive antigen {ECO:0000313|EMBL:KRM40298.1};", XP055645697, retrieved from UniProt Database accession no. A0A0R1YDA1
DATABASE UniProt KB 20 December 2017 (2017-12-20), "SubName: Full=Oleate hydratase {ECO:0000313|EMBL:ASY54083.1};", XP055645702, retrieved from UniProt Database accession no. A0A249NK64
DATABASE UniProt KB 22 February 2012 (2012-02-22), "SubName: Full=67 kDa myosin-cross-reactive antigen like family protein {ECO:0000313|EMBL:AEV95783.1};", XP055645703, retrieved from UniProt Database accession no. G8PAJ4
DATABASE UniProt KB 20 January 2016 (2016-01-20), "SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:KRO25982.1};", XP055645706, retrieved from UniProt Database accession no. A0A0R2NJM0
DATABASE UniProt KB 1 March 2003 (2003-03-01), "Q8DT14 SubName: Full=Putative 67 kDa myosin-crossreactive streptococcal antigen-like protein {ECO:0000313|EMBL:AAN59227.1};", XP055645710, retrieved from UniProt Database accession no. Q8DT14
DATABASE UniProt KB 28 March 2018 (2018-03-28), "SubName: Full=Oleate hydratase {ECO:0000313|EMBL:PNM00509.1};", XP055645713, retrieved from UniProt Database accession no. A0A2J9QD48
DATABASE UniProt KB 12 April 2017 (2017-04-12), "SubName: Full=Oleate hydratase {ECO:0000313|EMBL:SDO45867.1};", XP055645718, retrieved from UniProt Database accession no. A0A1H0JQ81
DATABASE UniProt KB 25 October 2017 (2017-10-25), "SubName: Full=Oleate hydratase {ECO:0000313|EMBL:SNU06541.1};", XP055645719, retrieved from UniProt Database accession no. A0A239R7F6
DATABASE Protein 20 November 2015 (2015-11-20), "oleate hydratase [Lactobacillus acidophilus]", XP055645725, retrieved from NCBI Database accession no. WP_003546333
KANG WOO-RI ET AL.: "Production of δ-decalactone from linoleic acid via 13-hydroxy-9(Z)- octadecenoic acid intermediate by one-pot reaction using linoleate 13-hydratase and whole Yarrowia lipolytica cells", BIOTECHNOL LETT, vol. 38, 2016, pages 817 - 823, XP035901465
J PARK ET AL., J. BIOTECHNOL., vol. 208, 2015, pages 1 - 10
H OH ET AL., ADV. SYNTH. CATAL., vol. 357, 2015, pages 408 - 416
YY CHEN ET AL., FRONT MICROBIOL., vol. 7, 2016, pages e1561
K KIM ET AL., BIOTECH. BIOENG., vol. 112, 2015, pages 2206 - 2213
ALINA SWIZDOR ET AL., INT. J. MOL. SCI., vol. 13, 2012, pages 16514 - 16543
ALAN VILLALOBOS ET AL.: "Gene Designer: a synthetic biology tool for constructing artificial DNA segments", BMC BIOINFORMATICS, vol. 7, 6 June 2006 (2006-06-06), pages 285, XP002458330, DOI: 10.1186/1471-2105-7-285
XIE ET AL., FOOD CHEM., vol. 117, 2009, pages 375 - 380
SAKAYU SHIMIZU, BIOCHEM. BIOPHYS. RES. COMMUN., vol. 91, 1979, pages 108 - 113
PARK ET AL., J BIOTECHNOL, vol. 208, 2015, pages 1 - 10
WACHE ET AL., APPL. ENVIRON. MICROBIOL., vol. 66, 2000, pages 1233 - 1236
DATABASE UniProt KB 20 January 2016 (2016-01-20), "SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:KRL21052.1};", XP055645679, retrieved from UniProt Database accession no. AOAOR1NM81
DATABASE Uniprot KB 25 October 2017 (2017-10-25), "SubName: Full=Oleate hydratase {ECO:0000313|EMBL:OXC20804.1};", XP055645687, Database accession no. A0A226SF70
DATABASE Uniprot KB 2 November 2016 (2016-11-02), "SubName: Full=Oleate hydratase {ECO:0000313|EMBL:OCX10702.1};", XP055645689, retrieved from UniProt Database accession no. A0A1C2D7G5
DATABASE UniProt KB 25 October 2017 (2017-10-25), "SubName: Full=Oleate hydratase {ECO:0000313|EMBL:OXC20121.1};", XP055645692, retrieved from UniProt Database accession no. A0A226SCJ9
DATABASE UniProt KB 20 January 2016 (2016-01-20), "SubName: Full=Myosin-cross-reactive antigen {ECO:0000313|EMBL:KRM40298.1};", XP055645697, retrieved from UniProt Database accession no. A0A0R1YDA1
DATABASE UniProt KB 20 December 2017 (2017-12-20), "SubName: Full=Oleate hydratase {ECO:0000313|EMBL:ASY54083.1};", XP055645702, retrieved from UniProt Database accession no. A0A249NK64
DATABASE UniProt KB 22 February 2012 (2012-02-22), "SubName: Full=67 kDa myosin-cross-reactive antigen like family protein {ECO:0000313|EMBL:AEV95783.1};", XP055645703, retrieved from UniProt Database accession no. G8PAJ4
DATABASE UniProt KB 20 January 2016 (2016-01-20), "SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:KRO25982.1};", XP055645706, retrieved from UniProt Database accession no. A0A0R2NJM0
DATABASE UniProt KB 1 March 2003 (2003-03-01), "Q8DT14 SubName: Full=Putative 67 kDa myosin-crossreactive streptococcal antigen-like protein {ECO:0000313|EMBL:AAN59227.1};", XP055645710, retrieved from UniProt Database accession no. Q8DT14
DATABASE UniProt KB 28 March 2018 (2018-03-28), "SubName: Full=Oleate hydratase {ECO:0000313|EMBL:PNM00509.1};", XP055645713, retrieved from UniProt Database accession no. A0A2J9QD48
DATABASE UniProt KB 12 April 2017 (2017-04-12), "SubName: Full=Oleate hydratase {ECO:0000313|EMBL:SDO45867.1};", XP055645718, retrieved from UniProt Database accession no. A0A1H0JQ81
DATABASE UniProt KB 25 October 2017 (2017-10-25), "SubName: Full=Oleate hydratase {ECO:0000313|EMBL:SNU06541.1};", XP055645719, retrieved from UniProt Database accession no. A0A239R7F6
DATABASE Protein 20 November 2015 (2015-11-20), "oleate hydratase [Lactobacillus acidophilus]", XP055645725, retrieved from NCBI Database accession no. WP_003546333
KANG WOO-RI ET AL.: "Production of δ-decalactone from linoleic acid via 13-hydroxy-9(Z)- octadecenoic acid intermediate by one-pot reaction using linoleate 13-hydratase and whole Yarrowia lipolytica cells", BIOTECHNOL LETT, vol. 38, 2016, pages 817 - 823, XP035901465
J PARK ET AL., J. BIOTECHNOL., vol. 208, 2015, pages 1 - 10
H OH ET AL., ADV. SYNTH. CATAL., vol. 357, 2015, pages 408 - 416
YY CHEN ET AL., FRONT MICROBIOL., vol. 7, 2016, pages e1561
K KIM ET AL., BIOTECH. BIOENG., vol. 112, 2015, pages 2206 - 2213
ALINA SWIZDOR ET AL., INT. J. MOL. SCI., vol. 13, 2012, pages 16514 - 16543
ALAN VILLALOBOS ET AL.: "Gene Designer: a synthetic biology tool for constructing artificial DNA segments", BMC BIOINFORMATICS, vol. 7, 6 June 2006 (2006-06-06), pages 285, XP002458330, DOI: 10.1186/1471-2105-7-285
XIE ET AL., FOOD CHEM., vol. 117, 2009, pages 375 - 380
SAKAYU SHIMIZU, BIOCHEM. BIOPHYS. RES. COMMUN., vol. 91, 1979, pages 108 - 113
PARK ET AL., J BIOTECHNOL, vol. 208, 2015, pages 1 - 10
WACHE ET AL., APPL. ENVIRON. MICROBIOL., vol. 66, 2000, pages 1233 - 1236
Attorney, Agent or Firm:
SAKAI INTERNATIONAL PATENT OFFICE (JP)
Download PDF:
Previous Patent: PHOTOSENSITIVE RESIN COMPOSITION AND PATTERN STRUCTURE
Next Patent: POWER STORAGE MODULE
Next Patent: POWER STORAGE MODULE