Title:
METHOD FOR PRODUCING Nε-ACYL-L-LYSINE
Document Type and Number:
WIPO Patent Application WO/2017/082374
Kind Code:
A1
Abstract:
The present invention provides a method for producing Nε-dodecanoyl-L-lysine, said method using an enzyme that performs appropriately for the industrial production of Nε-dodecanoyl-L-lysine. Specifically, the present invention provides a method for producing Nε-acyl-L-lysine wherein Nε-acyl-L-lysine is generated by reacting a carboxylic acid or a salt thereof and L-lysine or a salt thereof in the presence of one of the following proteins (A)-(C): (A) a protein that includes an amino acid sequence of SEQ ID NO: 1; (B) a protein that has an Nε-acyl-L-lysine-specific aminoacylase activity and includes an amino acid sequence in which one or a plurality of amino acid residues is/are inserted, added, deleted, or replaced in the amino acid sequence of SEQ ID NO: 1; or (C) a protein that has an Nε-acyl-L-lysine-specific aminoacylase activity and includes an amino acid sequence that demonstrates 90% or more homology with the amino acid sequence of SEQ ID NO: 1.
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Inventors:
TAKAKURA JUN (JP)
NOZAKI HIROYUKI (JP)
NOZAKI HIROYUKI (JP)
Application Number:
PCT/JP2016/083457
Publication Date:
May 18, 2017
Filing Date:
November 11, 2016
Export Citation:
Assignee:
AJINOMOTO KK (JP)
International Classes:
C12P13/08; C12N15/09
Domestic Patent References:
| WO2010067871A1 | 2010-06-17 | |||
| WO2006088199A1 | 2006-08-24 | |||
| WO2006088199A1 | 2006-08-24 | |||
| WO2010067871A1 | 2010-06-17 | |||
| WO2005103278A1 | 2005-11-03 | |||
| WO2002081694A1 | 2002-10-17 | |||
| WO2001023491A1 | 2001-04-05 |
Foreign References:
| JP2003210164A | 2003-07-29 | |||
| JP2004081107A | 2004-03-18 | |||
| JPH09322774A | 1997-12-16 |
Other References:
DATABASE Database UniProt/ GeneSeq [online] 11 December 2013 (2013-12-11), Database accession no. ACY49244
KOREISHI M. ET AL.: "Efficient Nepsilon-lauroyl-L- lysine production by recombinant epsilon-lysine acylase from Streptomyces mobaraensis", J. BIOTECHNOL., vol. 141, 2009, pages 160 - 165, XP026099302
CHIBATA I. ET AL.: "Studies on amino acids. XIII. A survey of the epsilon-lysine acylase activity in microorganisms", BULL. AGR. CHEM. SOC. JAPAN, vol. 24, no. 1, 1960, pages 31 - 36
PRO. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873
KARLIN; ALTSCCHUL, METHODS ENZYMOL., vol. 183, 1990, pages 63
J. MOL. BIOL., vol. 48, 1970, pages 443 - 453
ALAN VILLALOBOS ET AL.: "Gene Designer: a synthetic biology tool for constructing artificial DNA segments", BMC BIOINFORMATICS, vol. 7, 6 June 2006 (2006-06-06), pages 285, XP021013796, DOI: doi:10.1186/1471-2105-7-285
INT. J. SYST. BACTERIOL., vol. 41, 1981, pages 255
"NCBI", Database accession no. WP 012844854.1
NAKAMURA, Y. ET AL., NUCL. ACIDS RES., vol. 28, 2000, pages 292
JAOCS, vol. 82, no. 9, 2005
KOREISHI M. ET AL.: "Efficient Nepsilon-lauroyl-L- lysine production by recombinant epsilon-lysine acylase from Streptomyces mobaraensis", J. BIOTECHNOL., vol. 141, 2009, pages 160 - 165, XP026099302
CHIBATA I. ET AL.: "Studies on amino acids. XIII. A survey of the epsilon-lysine acylase activity in microorganisms", BULL. AGR. CHEM. SOC. JAPAN, vol. 24, no. 1, 1960, pages 31 - 36
PRO. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873
KARLIN; ALTSCCHUL, METHODS ENZYMOL., vol. 183, 1990, pages 63
J. MOL. BIOL., vol. 48, 1970, pages 443 - 453
ALAN VILLALOBOS ET AL.: "Gene Designer: a synthetic biology tool for constructing artificial DNA segments", BMC BIOINFORMATICS, vol. 7, 6 June 2006 (2006-06-06), pages 285, XP021013796, DOI: doi:10.1186/1471-2105-7-285
INT. J. SYST. BACTERIOL., vol. 41, 1981, pages 255
"NCBI", Database accession no. WP 012844854.1
NAKAMURA, Y. ET AL., NUCL. ACIDS RES., vol. 28, 2000, pages 292
JAOCS, vol. 82, no. 9, 2005
Attorney, Agent or Firm:
SAKAI INTERNATIONAL PATENT OFFICE (JP)
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