Title:
METHOD FOR PRODUCING CAPSID PROTEIN OF ADENO-ASSOCIATED VIRUS, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/067418
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a method for producing adeno-associated viral capsid protein useful as an antigen for the production of anti-AAV antibodies. The present invention provides a method for producing adeno-associated viral capsid protein, comprising (a) a step of producing adeno-associated viral capsid protein in bacteria; (b) a step of recovering the protein as an insoluble protein; and (c) a step of solubilizing the recovered insoluble protein.
Inventors:
YAURA HISAKO (JP)
NISHIHACHIJO MASAKATSU (JP)
HIRAYAMA YUSUKE (JP)
NISHIHACHIJO MASAKATSU (JP)
HIRAYAMA YUSUKE (JP)
Application Number:
PCT/JP2019/038137
Publication Date:
April 02, 2020
Filing Date:
September 27, 2019
Export Citation:
Assignee:
KANEKA CORP (JP)
International Classes:
C12P21/02; C07K1/22; C07K14/015; C07K16/08; C07K17/02; C12N5/0781; C12N15/35
Foreign References:
| US20090075357A1 | 2009-03-19 | |||
| US20180169273A1 | 2018-06-21 | |||
| US20180179265A1 | 2018-06-28 | |||
| US20140024013A1 | 2014-01-23 | |||
| CN107365364A | 2017-11-21 |
Other References:
WANG, A. P. ET AL.: "Prokaryotic expression of VP3 gene of avian adeno-associated virus and antiserum preparation", ZHONGGUO SHOUYI KEXUE, vol. 43, no. 12, 2013, pages 1291 - 1294
WANG, J. ET AL.: "Prokaryotic expression of the Rep78 and VP3 proteins of avian adeno-associated virus and preparation of specific antisera", ZHONGGUO SHOUYI XUEBAO, vol. 28, no. 12, 2008, pages 1363 - 1366
RUSSE LL, B. L. ET AL.: "Solubilisation and purification of recombinant bluetongue virus VP7 expressed in a bacterial system", PROTEIN EXPR. PURIF., vol. 147, July 2018 (2018-07-01), pages 85 - 93, XP085381310, DOI: 10.1016/j.pep.2018.03.006
KLEID, D. G. ET AL.: "Cloned viral protein vaccine for foot-and-mouth disease: responses in cattle and swine", SCIENCE, vol. 214, 1981, pages 1125 - 1129, XP55701656
WANG, J. ET AL.: "Prokaryotic expression of the Rep78 and VP3 proteins of avian adeno-associated virus and preparation of specific antisera", ZHONGGUO SHOUYI XUEBAO, vol. 28, no. 12, 2008, pages 1363 - 1366
RUSSE LL, B. L. ET AL.: "Solubilisation and purification of recombinant bluetongue virus VP7 expressed in a bacterial system", PROTEIN EXPR. PURIF., vol. 147, July 2018 (2018-07-01), pages 85 - 93, XP085381310, DOI: 10.1016/j.pep.2018.03.006
KLEID, D. G. ET AL.: "Cloned viral protein vaccine for foot-and-mouth disease: responses in cattle and swine", SCIENCE, vol. 214, 1981, pages 1125 - 1129, XP55701656
Attorney, Agent or Firm:
SIKS & CO. (JP)
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