Title:
METHOD FOR PRODUCING CELL POPULATION IN WHICH PERIODONTAL LIGAMENT STEM CELLS ARE CONCENTRATED
Document Type and Number:
WIPO Patent Application WO/2019/074046
Kind Code:
A1
Abstract:
[Problem] The purpose of the present invention is to provide a method for preparing a cell population containing numerous periodontal ligament stem cells, particularly epithelial stem cells from the periodontal ligament and/or periodontal mesenchymal stem cells from the periodontal ligament. [Solution] Provided is a method for producing a cell population in which periodontal ligament stem cells are concentrated, said method including a stem cell selection step in which a cell population that has been isolated from periodontal ligament is cultivated in a culture medium containing a prescribed proliferation factor. Further provided are: a cell population in which periodontal ligament stem cells are concentrated; a pharmaceutical composition having said cell population or the culture supernatant thereof as an active ingredient; a method for producing a dental implant or tooth having a periodontal ligament coating formed using the cell population; and a novel marker for periodontal ligament stem cells.
Inventors:
OKUBO NAOTO (JP)
Application Number:
PCT/JP2018/037900
Publication Date:
April 18, 2019
Filing Date:
October 11, 2018
Export Citation:
Assignee:
UNIV HOKKAIDO NAT UNIV CORP (JP)
International Classes:
C12N5/074; A61K35/32; A61L27/38; A61P1/02; C07K14/705; C07K14/485; C07K14/50; C12N15/09
Other References:
ATHANASSIOU-PAPAEFTHYMIIOU, M. ET AL.: "Isolation and Characterization of Human Adult Epithelial Stem Cells from the Periodontal Ligament", JOURNAL OF DENTAL RESEARCH, vol. 94, no. 11, 2015, pages 1591 - 1600, XP055594443
KOJIMA, Y. ET AL.: "Periodontal regeneration and FGF-2", INFLAMMATION AND REGENERATION, vol. 33, no. 1, 2013, pages 72 - 77
TRUBIANI, 0. ET AL.: "Assessment of an Efficient Xeno-Free Culture System of Human Periodontal Ligament Stem Cells", TISSUE ENGINEERING: PART C, vol. 21, no. 1, 2015, pages 52 - 64, XP055401951, DOI: doi:10.1089/ten.tec.2014.0024
XIONG, J. ET AL.: "Epithelial Cell Rests of Malassez Contain Unique Stem Cell Populations Capable of Undergoing Epithelial-Mesenchymal Transition", STEM CELLS AND DEVELOPMENT, vol. 21, no. 11, 2012, pages 2012 - 2025, XP055594448
KAKEN 2015 RF#, 6 January 2017 (2017-01-06), Retrieved from the Internet [retrieved on 20181102]
CHAVEZ, M. G. ET AL.: "Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor", JOURNAL OF VISUALIZED EXPERIMENTS, vol. 87, 2014, pages 1 - 7, XP055594453
KADOKURA, HIROSHI ET AL.: "Examine potential of FGF in regenerative therapy of dental clinic area", JOURNAL OF THE ACADEMY OF GNATHOLOGY AND OCCLUSION, vol. 22, no. 2, 2001, pages 210 - 216
YAN, M. ET AL.: "G Protein-Coupled Receptor 87 (GPR87) Promotes the Growth and Metastasis of CD 133+ Cancer Stem-Like Cells in Hepatocellular Carcinoma", PLOS ONE, vol. 8, no. 4, April 2013 (2013-04-01), pages 1 - 10, XP055594456
TAKEO, M. ET AL.: "EdnrB governs regenerative response of melanocyte stem cells by crosstalk with Wnt signaling", CELL REP., vol. 15, no. 6, May 2016 (2016-05-01), pages 1291 - 1302, XP055594461, DOI: 10.1016/j.celrep.2016.04.006
KIMURA, H. ET AL.: "Role of DNA methy lat ion for expression of novel stem cell marker CDCP1 inhematopoietic cells", LEUKEMIA, vol. 20, no. 9, 2006, pages 1551 - 1556, XP055594462, DOI: 10.1038/sj.leu.2404312
GAO, B. ET AL.: "BMP7 and EREG Contribute to the Inductive Potential of Dental Mesenchyme", SCIENTIFIC REPORTS, vol. 5, no. 09903, May 2015 (2015-05-01), pages 1 - 10, XP055594465
ZIEGLER, B. L. ET AL.: "KDR Receptor: A Key Marker Defning Hematopoietic Stem Cells", SCIENCE, vol. 285, 1999, pages 1553 - 1558, XP002195471
RAMAN, J. D. ET AL.: "Decreased expression of the human stem cell marker, Rex-1 (zfp-42), in renal cell carcinoma", CARCINOGENESIS, vol. 27, no. 3, 2006, pages 499 - 507, XP055594467, ISSN: 0143-3334, DOI: 10.1093/carcin/bgi299
KOJIMA, Y. ET AL.: "Periodontal regeneration and FGF-2", INFLAMMATION AND REGENERATION, vol. 33, no. 1, 2013, pages 72 - 77
TRUBIANI, 0. ET AL.: "Assessment of an Efficient Xeno-Free Culture System of Human Periodontal Ligament Stem Cells", TISSUE ENGINEERING: PART C, vol. 21, no. 1, 2015, pages 52 - 64, XP055401951, DOI: doi:10.1089/ten.tec.2014.0024
XIONG, J. ET AL.: "Epithelial Cell Rests of Malassez Contain Unique Stem Cell Populations Capable of Undergoing Epithelial-Mesenchymal Transition", STEM CELLS AND DEVELOPMENT, vol. 21, no. 11, 2012, pages 2012 - 2025, XP055594448
KAKEN 2015 RF#, 6 January 2017 (2017-01-06), Retrieved from the Internet
CHAVEZ, M. G. ET AL.: "Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor", JOURNAL OF VISUALIZED EXPERIMENTS, vol. 87, 2014, pages 1 - 7, XP055594453
KADOKURA, HIROSHI ET AL.: "Examine potential of FGF in regenerative therapy of dental clinic area", JOURNAL OF THE ACADEMY OF GNATHOLOGY AND OCCLUSION, vol. 22, no. 2, 2001, pages 210 - 216
YAN, M. ET AL.: "G Protein-Coupled Receptor 87 (GPR87) Promotes the Growth and Metastasis of CD 133+ Cancer Stem-Like Cells in Hepatocellular Carcinoma", PLOS ONE, vol. 8, no. 4, April 2013 (2013-04-01), pages 1 - 10, XP055594456
TAKEO, M. ET AL.: "EdnrB governs regenerative response of melanocyte stem cells by crosstalk with Wnt signaling", CELL REP., vol. 15, no. 6, May 2016 (2016-05-01), pages 1291 - 1302, XP055594461, DOI: 10.1016/j.celrep.2016.04.006
KIMURA, H. ET AL.: "Role of DNA methy lat ion for expression of novel stem cell marker CDCP1 inhematopoietic cells", LEUKEMIA, vol. 20, no. 9, 2006, pages 1551 - 1556, XP055594462, DOI: 10.1038/sj.leu.2404312
GAO, B. ET AL.: "BMP7 and EREG Contribute to the Inductive Potential of Dental Mesenchyme", SCIENTIFIC REPORTS, vol. 5, no. 09903, May 2015 (2015-05-01), pages 1 - 10, XP055594465
ZIEGLER, B. L. ET AL.: "KDR Receptor: A Key Marker Defning Hematopoietic Stem Cells", SCIENCE, vol. 285, 1999, pages 1553 - 1558, XP002195471
RAMAN, J. D. ET AL.: "Decreased expression of the human stem cell marker, Rex-1 (zfp-42), in renal cell carcinoma", CARCINOGENESIS, vol. 27, no. 3, 2006, pages 499 - 507, XP055594467, ISSN: 0143-3334, DOI: 10.1093/carcin/bgi299
Attorney, Agent or Firm:
IP-ASSIST PATENT OFFICE (JP)
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