Title:
METHOD FOR PRODUCING CULTURED CELLS
Document Type and Number:
WIPO Patent Application WO/2020/184403
Kind Code:
A1
Abstract:
In one embodiment, the present invention provides a method wherein, with regard to a plurality of cells, all of the cells are not subcultured but a part thereof are screened so that a part of the cells can be selected on the basis of the screening result. In one embodiment, the present invention relates to a method for producing cultured cells, said method comprising: a) a step for culturing a plurality of animal cells or plant cells in a culture container by adhesion culture or on a semisolid culture medium to thereby form multiple colonies originated from a single cell; b) a step for, with regard to a part of the multiple colonies, detecting one or more base sequences in a nucleic acid by a nucleic acid- or protein-detection method, wherein the multiple colonies are cultured in the culture container during the detection period; and c) a step for selecting and collecting the part of the colonies on the basis of the detection result.
Inventors:
YOKOUCHI YUJI (JP)
ERA TAKUMI (JP)
SUZUKI SHINICHI (JP)
ERA TAKUMI (JP)
SUZUKI SHINICHI (JP)
Application Number:
PCT/JP2020/009554
Publication Date:
September 17, 2020
Filing Date:
March 06, 2020
Export Citation:
Assignee:
UNIV FUKUSHIMA MEDICAL (JP)
ZENOAQ RESOURCE CO LTD (JP)
ZENOAQ RESOURCE CO LTD (JP)
International Classes:
C12N5/04; A61K35/12; C12N5/074; C12N5/10; C12N15/09; C12Q1/6883
Foreign References:
| JP2019506170A | 2019-03-07 |
Other References:
TAKARA BIO INC.,: "Handbook for Gene Editing Experiment", 22 February 2019 (2019-02-22), pages 14, Retrieved from the Internet [retrieved on 20200513]
"DNA polymerase that detects single base mismatch at the 3'-terminal, HiDi DNA Polymerase", FUNAKOSHI, 21 January 2016 (2016-01-21), XP055739897, Retrieved from the Internet [retrieved on 20200513]
AGUDELO, D. ET AL.: "Marker-free coselection for CRISPR-driven genome editing in human cells", NATURE METHODS, vol. 14, no. 6, 2017, pages 615 - 620, XP055473803, DOI: 10.1038/nmeth.4265
FUJITA, TOSHITSUGU ET AL.: "Detection of genome-edited cells using ORNi-PCR", ON-LINE ABSTRACTS OF THE 41ST ANNUAL ACADEMIC CONFERENCE OF THE MOLECULAR BIOLOGY SOCIETY OF JAPAN, 9 November 2018 (2018-11-09)
"DNA polymerase that detects single base mismatch at the 3'-terminal, HiDi DNA Polymerase", FUNAKOSHI, 21 January 2016 (2016-01-21), XP055739897, Retrieved from the Internet
AGUDELO, D. ET AL.: "Marker-free coselection for CRISPR-driven genome editing in human cells", NATURE METHODS, vol. 14, no. 6, 2017, pages 615 - 620, XP055473803, DOI: 10.1038/nmeth.4265
FUJITA, TOSHITSUGU ET AL.: "Detection of genome-edited cells using ORNi-PCR", ON-LINE ABSTRACTS OF THE 41ST ANNUAL ACADEMIC CONFERENCE OF THE MOLECULAR BIOLOGY SOCIETY OF JAPAN, 9 November 2018 (2018-11-09)
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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