METHOD FOR PRODUCING GENOME-MODIFIED PLANTS FROM PLANT PROTOPLASTS AT HIGH EFFICIENCY

WIPO Patent Application WO/2017/061805

A1

The present invention relates to a method for increasing the production efficiency of genome-modified plants regenerated from plant protoplasts by using a Cas protein-guide RNA ribonucleoprotein (RNP). The method for increasing the production efficiency of genome-modified plants of the present invention can efficiently produce plants with mutated target genes, and can also minimize the insertion of foreign DNA into plants. Therefore, the present invention can be very usefully used in various fields such as agriculture, food and biotechnology fields.

KIM JIN SOO (KR)
KIM JUNGEUN (KR)
CHOE SUNGHWA (KR)
WOO JE WOOK (KR)
KWON SOON IL (KR)
KIM HYERAN (KR)

PCT/KR2016/011216

April 13, 2017

October 06, 2016

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INST BASIC SCIENCE (KR)
UNIV SEOUL NAT R & DB FOUND (KR)
AICT (KR)

C12N15/82; A01H5/12; C12N9/22

CN102618554A	2012-08-01
US20140295556A1	2014-10-02
US20090013433A1	2009-01-08
KR20160011217A	2016-01-29

HYUN ET AL.: "Site-directed Mutagenesis in Arabidopsis Thaliana Using Dividing Tissue-targeted RGEN of the CRISPR/Cas System to Generate Heritable Null Alleles", PLANTA, vol. 241, 2015, pages 271 - 284, XP035417502
YANG ET AL.: "The Mechanisms of Brassinosteroids Action: from Signal Transduction to Plant Development", MOLECULAR PLANT, vol. 4, no. 4, July 2011 (2011-07-01), pages 588 - 600, XP055373079
See also references of EP 3366777A4

SON, Min (KR)

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