METHOD FOR PRODUCING HIGH-TITER VIRAL VECTOR - NIPPON MEDICAL SCHOOL FOUNDATION

Title:

METHOD FOR PRODUCING HIGH-TITER VIRAL VECTOR

Document Type and Number:

WIPO Patent Application WO/2023/243679

Kind Code:

A1

Abstract:

The problem addressed by the present invention is to provide a viral vector with a higher infectious titer for application to gene and cell therapies.　The present invention relates to a method for producing a viral vector that includes a step that performs treatment to synchronize the cell cycle of the cells to the G2/M phase prior to introducing a plasmid that contains a target gene into cells for virus production.

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Inventors:

YAMAZAKI YOSHIYUKI (JP)
MIYAGAWA YOSHITAKA (JP)
NITO CHIKAKO (JP)
HAYASHI MASAHIRO (JP)
NAKAISHI TOMOYUKI (JP)

Application Number:

PCT/JP2023/022216

Publication Date:

December 21, 2023

Filing Date:

June 15, 2023

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Assignee:

NIPPON MEDICAL SCHOOL FOUNDATION (JP)
KANEKA CORP (JP)

International Classes:

C12N15/86; C12N5/071; C12N5/074; C12N5/0775; C12N5/09; C12N15/861; C12N15/864; C12N15/867

Domestic Patent References:

WO2000078987A1

2000-12-28

Foreign References:

JP2018531610A	2018-11-01
JP2006509524A	2006-03-23

Other References:

GROSCHEL BETTINA, BUSHMAN FREDERIC: "Cell Cycle Arrest in G 2 /M Promotes Early Steps of Infection by Human Immunodeficiency Virus", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 79, no. 9, 1 May 2005 (2005-05-01), US , pages 5695 - 5704, XP093117552, ISSN: 0022-538X, DOI: 10.1128/JVI.79.9.5695-5704.2005
MENGFEI CHEN, JINGJING HUANG, XUEJIAO YANG, BINGQIAN LIU, WEIZHONG ZHANG, LI HUANG, FEI DENG, JIAN MA, YUJING BAI, RONG LU, BING H: "Serum Starvation Induced Cell Cycle Synchronization Facilitates Human Somatic Cells Reprogramming", PLOS ONE, vol. 7, no. 4, pages e28203, XP055715188, DOI: 10.1371/journal.pone.0028203
MIYAGAWA, YOSHITAKA: "Utilization of mesenchymal stem cells in gene cell therapy", JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, BIOMEDICAL DRUGS PUBLICATION, JP, vol. 272, no. 10, 7 March 2020 (2020-03-07), JP , pages 1069 - 1074, XP009551139, ISSN: 0039-2359

Attorney, Agent or Firm:

HIRAKI & ASSOCIATES (JP)

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