Title:
METHOD FOR PRODUCING LACTO-N-BIOSE I OR GALACTO-N-BIOSE
Document Type and Number:
WIPO Patent Application WO/2008/078614
Kind Code:
A1
Abstract:
Disclosed is a method for producing lacto-N-biose I or galacto-N-biose at low cost and in a simple manner. Specifically disclosed is a method for producing lacto-N-biose I or galacto-N-biose, which is characterized by reacting (i) a combination of a carbohydrate raw material and an enzyme capable of phosphorolyzing the carbohydrate raw material to produce α-glucose-1-phosphate with (ii) a combination of an enzyme capable of converting α-glucose-1-phosphate into UDP-glucose, an enzyme capable of converting UDP-galactose into galactose-1-phosphate and coenzymes for the enzymes, and/or an enzyme capable of converting α-glucose-1-phosphate and UDP-galactose into UDP-glucose and α-glucose-1-phosphate, respectively (an UDP-Gly-production enzyme) and a coenzyme for the enzyme, in the presence of N-acetylglucosamine or N-acetylgalactosamine, phosphoric acid, lacto-N-biose phosphorylase (EC 2.4.1.211) and UDP-glucose-4-epimerase (EC 5.1.3.2).
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Inventors:
KITAOKA MOTOMITSU (JP)
NISHIMOTO MAMORU (JP)
NISHIMOTO MAMORU (JP)
Application Number:
PCT/JP2007/074362
Publication Date:
July 03, 2008
Filing Date:
December 12, 2007
Export Citation:
Assignee:
INC ADMIN AGENCY NARO (JP)
KITAOKA MOTOMITSU (JP)
NISHIMOTO MAMORU (JP)
KITAOKA MOTOMITSU (JP)
NISHIMOTO MAMORU (JP)
International Classes:
C12P19/26; C12N15/09
Foreign References:
| JPH06253878A | 1994-09-13 | |||
| JP2003189891A | 2003-07-08 | |||
| JP2005341883A | 2005-12-15 | |||
| JP2006346470A | 2006-12-28 |
Other References:
FARKS E. ET AL.: "Enzymatic synthesis of Galbeta1 3GLnNAc derivatives utilising a phosphorylase from Bifidobacterium bifidum 20082", SYNLETT., no. 5, 2000, pages 728 - 730, XP008109825
KITAOKA M. ET AL.: "Novel putative galactose operon involving lacto-N-biose phosphorylase in Bifidobacterium longum", APPL. ENVIRON. MICROBIOL., vol. 71, no. 6, 2005, pages 3158 - 3162, XP008109882
NOGI Y.: "GAL3 gene product is required for maintenance of the induced state of the GAL cluster gene in Saccharomyces cerevisiae", J. BACTERIOL., vol. 165, no. 1, 1986, pages 101 - 106, XP008109827
LEVANDER F. ET AL.: "Enhanced exopolysaccharide production by metabolic engineering of Streptococcus thermophilus", APPL. ENVIR. MICROBIOL., vol. 68, no. 2, 2002, pages 784 - 790, XP008109881
SVENSSON M. ET AL.: "Metabolically improved exopolysaccharide production by Streptococcus thermophilus and its influence on the rheological properties of fermented milk", APPL. ENVIR. MICROBIOL., vol. 71, no. 10, 2005, pages 6398 - 6400, XP008109880
SAMBROOK ET AL.: "Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
ACTA CRYST., vol. D60, 2004, pages 1877 - 1878
ARCH. BIOCHEM. BIOPHYS., vol. 304, no. 2, 1993, pages 508 - 514
See also references of EP 2100967A4
KITAOKA M. ET AL.: "Novel putative galactose operon involving lacto-N-biose phosphorylase in Bifidobacterium longum", APPL. ENVIRON. MICROBIOL., vol. 71, no. 6, 2005, pages 3158 - 3162, XP008109882
NOGI Y.: "GAL3 gene product is required for maintenance of the induced state of the GAL cluster gene in Saccharomyces cerevisiae", J. BACTERIOL., vol. 165, no. 1, 1986, pages 101 - 106, XP008109827
LEVANDER F. ET AL.: "Enhanced exopolysaccharide production by metabolic engineering of Streptococcus thermophilus", APPL. ENVIR. MICROBIOL., vol. 68, no. 2, 2002, pages 784 - 790, XP008109881
SVENSSON M. ET AL.: "Metabolically improved exopolysaccharide production by Streptococcus thermophilus and its influence on the rheological properties of fermented milk", APPL. ENVIR. MICROBIOL., vol. 71, no. 10, 2005, pages 6398 - 6400, XP008109880
SAMBROOK ET AL.: "Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
ACTA CRYST., vol. D60, 2004, pages 1877 - 1878
ARCH. BIOCHEM. BIOPHYS., vol. 304, no. 2, 1993, pages 508 - 514
See also references of EP 2100967A4
Attorney, Agent or Firm:
HIRAKI, Yusuke et al. (3-20 Toranomon 4-chom, Minato-ku Tokyo 01, JP)
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