Title:
METHOD FOR PRODUCING PARVOVIRUS HAVING HIGH INFECTIVITY TITER
Document Type and Number:
WIPO Patent Application WO/2014/080676
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a method for producing a parvovirus having a high infectivity titer in a stable and simple manner. The problem can be solved by a method for producing a parvovirus having an infectivity titer as high as 108 TCID50/mL or more in a culture supernatant, said method comprising the steps of: inoculating a culture base material, which comprises a host cell at a cell density of 1/500 to 1/20 of the cell density achieved upon the proliferation of the host cell to confluent and a culture medium, with a seed virus of the parvovirus so that the multiplicity of infection becomes 0.0001 to 0.1; culturing the seed virus and the host cell for a time that is 5 to 11 times the doubling time of the host cell; and then collecting the culture supernatant.
Inventors:
YANAGIDA KOICHIRO (JP)
Application Number:
PCT/JP2013/073906
Publication Date:
May 30, 2014
Filing Date:
September 05, 2013
Export Citation:
Assignee:
ASAHI KASEI MEDICAL CO LTD (JP)
International Classes:
C12N7/00
Domestic Patent References:
| WO2011130119A2 | 2011-10-20 | |||
| WO2012007589A1 | 2012-01-19 | |||
| WO2007125605A1 | 2007-11-08 |
Foreign References:
| JP2010524482A | 2010-07-22 | |||
| JP2012503486A | 2012-02-09 | |||
| JPH02502876A | 1990-09-13 | |||
| JP2009297036A | 2009-12-24 | |||
| JPH02655876B | ||||
| JPS5822008A | 1983-02-09 | |||
| JPS6124370A | 1986-02-03 | |||
| JP3982843B2 | 2007-09-26 |
Other References:
See also references of EP 2924114A4
"Uirusu/Kansen new Fairu", 1997, YODOSHA CO., LTD., pages: 68
"Uirusu-gaku", 1997, ASAKURA PUBLISHING CO., LTD., pages: 222 - 223
M. AZETAKA, JPN. J. VET. SCI., vol. 43, 1980, pages 243 - 255
"Uirus Jikken Gaku Sohron", 1973, THE NATIONAL INSTITUTE OF HEALTH, pages: 61 - 180
GREGERSEN, J. P.: "Pharmazeutische Biotechnologie", 2000, pages: 257 - 281
P. A. BACHMANN, PROC. SOC. EXP. BIOL. MED., vol. 4, no. 140, 1972, pages 1369 - 1374
P. A. BACHMANN ET AL., ZBL. VET. MED. B., vol. 23, 1976, pages 355 - 363
"Uirus Jikken Gaku Kakuron", 1973, THE NATIONAL INSTITUTE OF HEALTH, pages: 22 - 23
WHO TECHNICAL REPORT, SERIES NO. 924, 2004, pages 162 - 165
WHO TECHNICAL REPORT, SERIES NO. 924, 2004, pages 163 - 164
"Seibutsukagaku Jikkenho 29", GAKKAI SHUPPAN CENTER, article "Dobutsu Saibo Baiyoho Nyumon", pages: 14 - 15
HIDEKI KOYAMA: "Saibo Baiyo Rabo Manyuaru", 1999, SPRINGER-VERLAG, pages: 51 - 52
TORU AKIYAMA: "Saibo/Baichi Katsuyo Handobukku", YODOSHA CO., LTD, pages: 45 - 46
"Medical Virology", 2000, NANKODO CO., LTD., pages: 171 - 172
VET. MICROBIOL., vol. 9, no. 2, 1984, pages 187 - 92
MICROBIOLOGICA, vol. 10, no. 3, 1987, pages 301 - 9
NIPPON JUIGAKU ZASSHI, vol. 50, no. 3, 1988, pages 803 - 8
NIPPON JUIGAKU ZASSHI., vol. 52, no. 2, 1990, pages 217 - 24
J. VET. MED. SCI., vol. 54, no. 2, 1992, pages 313 - 8
"Uirusu/Kansen new Fairu", 1997, YODOSHA CO., LTD., pages: 68
"Uirusu-gaku", 1997, ASAKURA PUBLISHING CO., LTD., pages: 222 - 223
M. AZETAKA, JPN. J. VET. SCI., vol. 43, 1980, pages 243 - 255
"Uirus Jikken Gaku Sohron", 1973, THE NATIONAL INSTITUTE OF HEALTH, pages: 61 - 180
GREGERSEN, J. P.: "Pharmazeutische Biotechnologie", 2000, pages: 257 - 281
P. A. BACHMANN, PROC. SOC. EXP. BIOL. MED., vol. 4, no. 140, 1972, pages 1369 - 1374
P. A. BACHMANN ET AL., ZBL. VET. MED. B., vol. 23, 1976, pages 355 - 363
"Uirus Jikken Gaku Kakuron", 1973, THE NATIONAL INSTITUTE OF HEALTH, pages: 22 - 23
WHO TECHNICAL REPORT, SERIES NO. 924, 2004, pages 162 - 165
WHO TECHNICAL REPORT, SERIES NO. 924, 2004, pages 163 - 164
"Seibutsukagaku Jikkenho 29", GAKKAI SHUPPAN CENTER, article "Dobutsu Saibo Baiyoho Nyumon", pages: 14 - 15
HIDEKI KOYAMA: "Saibo Baiyo Rabo Manyuaru", 1999, SPRINGER-VERLAG, pages: 51 - 52
TORU AKIYAMA: "Saibo/Baichi Katsuyo Handobukku", YODOSHA CO., LTD, pages: 45 - 46
"Medical Virology", 2000, NANKODO CO., LTD., pages: 171 - 172
VET. MICROBIOL., vol. 9, no. 2, 1984, pages 187 - 92
MICROBIOLOGICA, vol. 10, no. 3, 1987, pages 301 - 9
NIPPON JUIGAKU ZASSHI, vol. 50, no. 3, 1988, pages 803 - 8
NIPPON JUIGAKU ZASSHI., vol. 52, no. 2, 1990, pages 217 - 24
J. VET. MED. SCI., vol. 54, no. 2, 1992, pages 313 - 8
Attorney, Agent or Firm:
INABA, Yoshiyuki et al. (JP)
Yoshiyuki Inaba (JP)
Yoshiyuki Inaba (JP)
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