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Title:
METHOD FOR PRODUCING PEPTIDE CONTAINING NON-NATURAL AMINO ACID
Document Type and Number:
WIPO Patent Application WO/2021/117848
Kind Code:
A1
Abstract:
It has been found that in a translation system including a ribosome that includes modified L31 protein, by virtue of translating mRNA coding a peptide containing a non-natural amino acid, the translation amount of said peptide can be increased. It has also been found that the relative amount of by-products can also be reduced by using said ribosome. The modified L31 protein in the present invention has an amino acid sequence of wild Escherichia coli L31 protein with six or more amino acid residues from the C terminal deleted.

Inventors:
YOSHII SAKIKO (JP)
SHIMOMURA KOICHIRO (JP)
ISHINO SOU (JP)
Application Number:
PCT/JP2020/046213
Publication Date:
June 17, 2021
Filing Date:
December 11, 2020
Export Citation:
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Assignee:
CHUGAI PHARMACEUTICAL CO LTD (JP)
International Classes:
C12N15/31; C07K7/04; C07K7/64; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09; C12N15/11; C12N15/63; C12P21/02; C40B30/04; C40B40/10; G01N33/15; G01N33/50
Domestic Patent References:
WO2018174078A12018-09-27
WO2013100132A12013-07-04
WO2018143145A12018-08-09
WO2018225864A12018-12-13
WO2016148044A12016-09-22
WO2008117833A12008-10-02
WO2012074129A12012-06-07
WO2017150732A12017-09-08
WO2013100132A12013-07-04
WO2018225851A12018-12-13
WO2018143145A12018-08-09
WO2018225864A12018-12-13
WO2020138336A12020-07-02
Foreign References:
JP2004173627A2004-06-24
JPH078289A1995-01-13
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See also references of EP 4074829A4
Attorney, Agent or Firm:
HARUNA, Masao et al. (JP)
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