Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
METHOD FOR PRODUCTION OF MODIFIED PROTEIN
Document Type and Number:
WIPO Patent Application WO/2007/069604
Kind Code:
A1
Abstract:
Disclosed is a method for production of a modified protein comprising the step of modifying a substrate protein with an enzyme protein. In the method, the substrate protein and/or the enzyme protein is a chimeric protein having a protein-binding domain added thereto, wherein the protein-binding domain is capable of enhancing the affinity between the substrate protein and the enzyme protein. The method enables to achieve a protein-modification reaction with good efficiency by utilizing a protein-protein interaction. In the method, the affinity between a substrate protein and an enzyme can be increased by producing a protein-protein interaction between the substrate protein and the enzyme protein. In this manner, a substrate protein can be modified with a smaller amount of an enzyme with good efficiency.

More Like This:
WO/1989/006276HIV-2-TYPE RETROVIRUSES OF PRIMATES, VACCINES, DIAGNOSTIC AND PHARMACEUTICAL COMPOSITIONS
JPS6485093PROTEIN ARP HAVING IMMUNOGLOBULIN A BINDING ACTIVITY, CLONING AND EXPRESSION OF THE PROTEIN, CORRESPONDING VECTOR AND HOST, REAGENT KIT AND MEDICINAL COMPOSITION
JP4356783A method for extracting biological substances from hair and a hair collecting device used for the method.
Inventors:
INAGAKI FUYUHIKO
KOBASHIGAWA YOSHIHIRO
NAITO MASATO
Application Number:
PCT/JP2006/324761
Publication Date:
June 21, 2007
Filing Date:
December 12, 2006
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
UNIV HOKKAIDO NAT UNIV CORP (JP)
INAGAKI FUYUHIKO
KOBASHIGAWA YOSHIHIRO
NAITO MASATO
International Classes:
C12P21/00; C07K19/00; C12N15/09; C12N9/12
Foreign References:
JP2003185655A2003-07-03
JPH05503430A1993-06-10
JPH0882624A1996-03-26
Other References:
KLEIN S. ET AL.: "Expression and purification of active PKB kinase from Escherichia coli", PROTEIN EXPR. PURIF., vol. 41, May 2005 (2005-05-01), pages 162 - 169, XP003014500
AGHAZADEH B. ET AL.: "Structural basis for relief of autoinhibition of the Db1 homology domain of proto-oncogene Vavby tyrosine phosphorylation", CELL, vol. 102, 2000, pages 625 - 633, XP003014111
Attorney, Agent or Firm:
SAEKI, Norio et al. (Aminosan Kaikan Building 15-8, Nihonbashi 3-chom, Chuo-ku Tokyo 27, JP)
Download PDF:
View/Download PDF      PDF Help



 
Previous Patent: PHYSIOLOGICALLY ACTIVE GLYCOLIPID

Next Patent: SUBSTRATE WITH BUILT-IN CHIP AND METHOD FOR MANUFACTURING SUBSTRATE WITH BUILT-IN CHIP