Title:
METHOD FOR PURIFYING FIBRINOGEN
Document Type and Number:
WIPO Patent Application WO/2017/123012
Kind Code:
A1
Abstract:
The present invention relates to a method for purifying fibrinogen and, more specifically, to a method for purifying fibrinogen, comprising the steps of: (a) (first glycine precipitation) precipitating fibrinogen, which is contained in a solution containing the fibrinogen, by adding glycine thereto until the glycine concentration becomes 1.5-2.5 M, removing a supernatant, and then obtaining a precipitate; (b) (second glycine precipitation) obtaining a lysis solution by lysing, in a lysis buffer, the precipitate obtained by the first glycine precipitation in step (a), precipitating the lysis solution by adding glycine thereto until the glycine concentration becomes 0.2-1.2 M, and recovering a supernatant; (c) (third glycine precipitation) precipitating the supernatant of step (b) by adding glycine thereto until the glycine concentration becomes 1.5-2.5 M, and obtaining a precipitate; and (d) obtaining a lysis solution by lysing, in a lysis buffer, the precipitate of step (c), and performing nanofiltration (NF) thereon by means of a nanofilter. According to the present invention, the novel method for purifying fibrinogen protein is a very safe method since the inflow of pathogenic animal-derived components (virus) is fundamentally intercepted, is very economical and efficient since high-purify fibrinogen protein having a high yield can be purified by a simple process, and can be useful for preventing and treating blood-related diseases, particularly, blood coagulation disorders since there is an advantage of increasing topical effects by having a high purity compared with that of fibrinogen purified by a conventional method.
Inventors:
KIM JUN SIC (KR)
KIM HYO JIN (KR)
PARK JI YOON (KR)
LEE JU HO (KR)
SON JAE WOON (KR)
SHIN YONG WON (KR)
KIM HYO JIN (KR)
PARK JI YOON (KR)
LEE JU HO (KR)
SON JAE WOON (KR)
SHIN YONG WON (KR)
Application Number:
PCT/KR2017/000389
Publication Date:
July 20, 2017
Filing Date:
January 12, 2017
Export Citation:
Assignee:
GREEN CROSS HOLDINGS CORP (KR)
International Classes:
C07K1/30; A61K38/36; B01J20/08; C07K1/34; C07K1/36; C07K14/75
Foreign References:
| US7442308B2 | 2008-10-28 | |||
| KR20130112031A | 2013-10-11 | |||
| CN104231072A | 2014-12-24 | |||
| KR20060043134A | 2006-05-15 |
Other References:
JILG ET AL.: "Isolation of Residual Fibrinogen from a Supernatant of Plasma Precipitated with 2.1 M Glycine", THROMBOSIS RESEARCH, vol. 9, no. 5, 1976, pages 479 - 489, XP055398910
See also references of EP 3404037A4
See also references of EP 3404037A4
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
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