METHOD FOR PURIFYING LOW-MOLECULAR WEIGHT FUCOSYLATED GLYCOSAMINOGLYCAN BY MEANS OF TANGENTIAL FLOW ULTRAFILTRATION

WIPO Patent Application WO/2022/062011

A1

Provided is a method for purifying low-molecular weight fucosylated glycosaminoglycan by means of tangential flow ultrafiltration. The method comprises the following steps: (1) dissolving a crude product in a certain proportion of water, and filtering same to obtain a mother solution; (2) installing a 30 kd membrane bag ultrafiltration system, and carrying out ultrafiltration on the mother solution, and then collecting the ultrafiltrate; (3) carrying out ultrafiltration by using a 3 kd membrane bag ultrafiltration system to remove small molecule organic and inorganic matter, and sampling trapped fluid; (4) carrying out ultrafiltration by using a 10 kd membrane ultrafiltration system, and collecting a percolate; and (5) finally, carrying out concentration by using the 3 kd membrane ultrafiltration system. The method provided by the invention has the advantages of a high efficiency, rapidness, mild conditions and a high repeatability, and provides a stable ultrafiltration process with a consistent product quality between batches for industrial production.

LI ZHENGUO (CN)
NAN ZHIYUAN (CN)
ZHENG SHUNLIANG (CN)
ZHOU JIANBO (CN)
HU JIEWEN (CN)
WANG YI (CN)
ZHANG YONGCHUN (CN)
WANG SIYAO (CN)
ZHUANG PING (CN)
JIA QINGBING (CN)

PCT/CN2020/122795

March 31, 2022

October 22, 2020

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JIUZHITANG CO LTD (CN)
MUDANJIANG YOUBO PHARMACEUTICAL CO LTD (CN)
HAINAN JIUZHITANG PHARMACEUTICAL CO LTD (CN)

A61P7/02; B01D61/14; C08B37/08

CN101735336A	2010-06-16
CN103936889A	2014-07-23
CN104130334A	2014-11-05
CN108285498A	2018-07-17
CN109251255A	2019-01-22
JPS63128001A	1988-05-31

LECOME INTELLECTUAL PROPERTY AGENT LTD. (CN)

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