Title:
METHOD FOR QUALITATIVELY TESTING ACIDIC OLIGOSACCHARIDES IN BREAST MILK
Document Type and Number:
WIPO Patent Application WO/2024/001248
Kind Code:
A1
Abstract:
A method for qualitatively testing acidic oligosaccharides in breast milk, comprising the following steps in sequence: (1) centrifuging breast milk, removing upper-layer fat and protein, and taking an intermediate layer of a centrifugal product; (2) adding ethanol, and performing standing and reaction; (3) centrifuging, and taking the supernatant; (4) adsorbing by using a chromatographic column, first washing by using ultrapure water, eluting by using a sodium chloride solution, and collecting an eluent; and (5) analyzing the eluent by means of liquid chromatography-mass spectrometry. By improving an extraction process, liquid chromatography and mass spectrometry parameters, nine acidic oligosaccharides derived from a breast milk sample can be accurately and qualitatively measured, and the method has good repeatability and is suitable for analysis of large-scale breast milk samples.
Inventors:
CHEN LIJUN (CN)
ZHANG MINGHUI (CN)
QIAO WEICANG (CN)
ZHAO JUNYING (CN)
JIA GE (CN)
WANG YALING (CN)
LIU YAN (CN)
CHEN JINGYAO (CN)
LIU QIAN (CN)
YANG BAOYU (CN)
YANG YIHAN (CN)
ZHANG MINGHUI (CN)
QIAO WEICANG (CN)
ZHAO JUNYING (CN)
JIA GE (CN)
WANG YALING (CN)
LIU YAN (CN)
CHEN JINGYAO (CN)
LIU QIAN (CN)
YANG BAOYU (CN)
YANG YIHAN (CN)
Application Number:
PCT/CN2023/078186
Publication Date:
January 04, 2024
Filing Date:
February 24, 2023
Export Citation:
Assignee:
BEIJING SANYUAN FOODS CO LTD (CN)
International Classes:
G01N30/02; G01N30/06; G01N30/14; G01N30/34; G01N30/72
Foreign References:
| CN115112799A | 2022-09-27 | |||
| CN109254101A | 2019-01-22 | |||
| CN107621399A | 2018-01-23 | |||
| CN109270176A | 2019-01-25 | |||
| CN110161147A | 2019-08-23 | |||
| CN113686992A | 2021-11-23 | |||
| JP2008185373A | 2008-08-14 | |||
| US20080009069A1 | 2008-01-10 | |||
| US20150013438A1 | 2015-01-15 |
Other References:
LI JIAQI, JIANG MAORONG, ZHOU JIAORUI, DING JUNJIE, GUO ZHIMOU, LI MING, DING FEI, CHAI WENGANG, YAN JINGYU, LIANG XINMIAO: "Characterization of rat and mouse acidic milk oligosaccharides based on hydrophilic interaction chromatography coupled with electrospray tandem mass spectrometry", CARBOHYDRATE POLYMERS, APPLIED SCIENCE PUBLISHERS , LTD BARKING, GB, vol. 259, 1 May 2021 (2021-05-01), GB , pages 117734, XP093122150, ISSN: 0144-8617, DOI: 10.1016/j.carbpol.2021.117734
JINGHUA WEI, LIJUN CHEN, ZHAO JUNYING, ZHANG YI, MENGNA JING, PIN WANG, JIANG TIEMIN, WEICANG QIAO: "Rapid and Simultaneous Detection of 4 Milk Oligosaccharides Using LC-MS", FOOD SCIENCE, BEIJING FOOD SCIENCE RESEARCH INSTITUTE, CN, vol. 37, no. 14, 11 March 2016 (2016-03-11), CN , pages 86 - 91, XP093122152, ISSN: 1002-6630, DOI: 10.7506/spkx1002-6630-201614015
JINGHUA WEI, LIJUN CHEN, ZHAO JUNYING, ZHANG YI, MENGNA JING, PIN WANG, JIANG TIEMIN, WEICANG QIAO: "Rapid and Simultaneous Detection of 4 Milk Oligosaccharides Using LC-MS", FOOD SCIENCE, BEIJING FOOD SCIENCE RESEARCH INSTITUTE, CN, vol. 37, no. 14, 11 March 2016 (2016-03-11), CN , pages 86 - 91, XP093122152, ISSN: 1002-6630, DOI: 10.7506/spkx1002-6630-201614015
Attorney, Agent or Firm:
BEIJING YUEHE INTELLECTUAL PROPERTY AGENCY CO., LTD et al. (CN)
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