METHOD FOR QUANTITATIVELY MEASURING INTERACTION BETWEEN MEMBRANE PROTEINS IN LIVING CELLS

Title:

METHOD FOR QUANTITATIVELY MEASURING INTERACTION BETWEEN MEMBRANE PROTEINS IN LIVING CELLS

Document Type and Number:

WIPO Patent Application WO/2018/048063

Kind Code:

A1

Abstract:

The present invention relates to a method for analyzing an interaction between a target membrane protein and a binding partner membrane protein for the target membrane protein in living cells. According to the present invention, in the method for analyzing an interaction between a target membrane protein and a binding partner membrane protein for the target membrane protein in living cells, the dissociation constant for the interaction can be calculated by treating cells to be analyzed with a capture probe for the target membrane protein to immobilize the target membrane protein on a substrate and measuring a change of the diffusion coefficient distribution of the binding partner membrane protein for the target membrane protein. Therefore, the interaction between the target membrane protein and the binding partner membrane protein for the target membrane protein can be quantitatively measured.

Inventors:

RYU SUNG HO (KR)
LEE NAM KI (KR)
KIM DOHYEON (KR)
PARK SOYEON (KR)
KIM DONG KYUN (KR)
KWON YONGHOON (KR)
JEONG MIN GYU (KR)

Application Number:

PCT/KR2017/005544

Publication Date:

March 15, 2018

Filing Date:

May 26, 2017

Export Citation:

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Assignee:

POSTECH ACAD IND FOUND (KR)

International Classes:

G01N33/68; G01N33/50; G01N33/58

Domestic Patent References:

WO2000017221A1

2000-03-30

Foreign References:

KR101600177B1	2016-03-07
US20100323957A1	2010-12-23
KR101568565B1	2015-11-23

Other References:

QIAN ET AL.: "Single Particle Tracking. Analysis of Diffusion and Flow in Two-dimensional Systems", BIOPHYSICAL JOURNAL, vol. 60, October 1991 (1991-10-01), pages 910 - 921, XP055487337
ZHU ET AL.: "Analysis of Two-dimensional Dissociation Constant of Laterally Mobile Ceil Adhesion Molecules", BIOPHYSICAL JOURNAL, vol. 92, 1 February 2007 (2007-02-01), pages 1022 - 1034, XP055487350

Attorney, Agent or Firm:

DANA PATENT LAW FIRM (KR)

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