Title:
METHOD, REAGENT AND KIT FOR QUANTIFYING CHOLESTEROL IN REMNANT-LIKE LIPOPROTEIN (RLP)
Document Type and Number:
WIPO Patent Application WO/2006/085654
Kind Code:
A1
Abstract:
It is intended to provide a quantification method whereby cholesterol in remnant-like lipoproteins can be conveniently and highly sensitively quantified without resorting to a procedure of separating a sample and a reagent and a kit therefor. A method for quantifying remnant-like lipoprotein cholesterol in a sample characterized by comprising, in an aqueous medium containing the sample and in the presence of a combination of specific surfactants and an enzyme hydrolyzing a phospholipid, reacting the remnant-like lipoprotein cholesterol in the sample with cholesterol ester hydrolase and cholesterol oxidase or cholesterol dehydrogenase (coexisting with an oxidized coenzyme) and then quantifying the hydrogen peroxide or the reduced coenzyme thus formed.
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Inventors:
MIYAUCHI KAZUHITO
FUJINAKA MAYUMI
FUJINAKA MAYUMI
Application Number:
PCT/JP2006/302528
Publication Date:
August 17, 2006
Filing Date:
February 14, 2006
Export Citation:
Assignee:
KYOWA MEDEX CO LTD (JP)
MIYAUCHI KAZUHITO
FUJINAKA MAYUMI
MIYAUCHI KAZUHITO
FUJINAKA MAYUMI
International Classes:
C12Q1/60; C12Q1/26; C12Q1/34; C12Q1/44; G01N21/78; G01N33/92
Domestic Patent References:
| WO2000043537A1 | 2000-07-27 | |||
| WO2003104486A1 | 2003-12-18 | |||
| WO2001094619A1 | 2001-12-13 | |||
| WO1996028734A1 | 1996-09-19 |
Foreign References:
| JP2001231597A | 2001-08-28 | |||
| JP2799835B2 | 1998-09-21 | |||
| JPH07301636A | 1995-11-14 | |||
| JPH08131197A | 1996-05-28 | |||
| JPH09285298A | 1997-11-04 |
Other References:
SAMMETT D. ET AL.: "Mechanisms of enhancement of cholesteryl ester transfer protein activity by lipolysis", J. BIOL. CHEM., vol. 260, no. 11, 10 June 1985 (1985-06-10), pages 6687 - 6697, XP008125014
MIYAZAWA Y.: "Explanation concerning the 2nd item, novel Insurance Coerage Examination enforced on and after October 01, 2005", THE JAPANESE JOURNAL OF CLINICAL PATHOLOGY, vol. 53, no. 12, 25 December 2005 (2005-12-25), pages 1180 - 1181, XP008121159
MIYAZAWA Y.: "Explanation concerning the 2nd item, novel Insurance Coerage Examination enforced on and after October 01, 2005", THE JAPANESE JOURNAL OF CLINICAL PATHOLOGY, vol. 53, no. 12, 25 December 2005 (2005-12-25), pages 1180 - 1181, XP008121159
Attorney, Agent or Firm:
Iwahashi, Kazuyuki (LTD. Intellectual Property Department 6-1,
Ohtemachi 1-chome,, Chiyoda-k, Tokyo 85, JP)
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