METHOD FOR REMOVING NON-TARGET RNA FROM RNA SAMPLE

WIPO Patent Application WO/2018/196763

A1

Provided is a method for removing a non-target RNA from an RNA sample, comprising: performing reverse transcription of an RNA sample by means of a reverse transcription primer, and removing the RNA template, so as to obtain a non-target first strand cDNA and a target first strand cDNA; hybridizing the non-target first strand cDNA with a specific probe to obtain a non-target first strand cDNA-probe complex; using a double-strand specific nuclease to digest the non-target first strand cDNA-probe complex to obtain a target first strand cDNA, the non-target first strand cDNA-probe complex being extended to obtain a double stranded DNA region that can be completely digested by the double-strand specific nuclease, and digestion being performed by adding the double-strand specific nuclease.

GENG LIANG (CN)
XIN WEN (CN)

PCT/CN2018/084323

November 01, 2018

April 25, 2018

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BEIJING TRANSGEN BIOTECH CO LTD (CN)

C12Q1/68; C12N15/10; C40B40/06

CN105297144A	2016-02-03
CN102533956A	2012-07-04
CN107119043A	2017-09-01

HANA YI.: "Duplex-specific nuclease efficiently removes rRNA for prokaryotic RNA-seq", NUCLEIC ACIDS RESEARCH, vol. 39, no. 20, 31 August 2011 (2011-08-31), pages E140, XP055092830, ISSN: 1362-4962
STUART KARCHER ET AL.: "Selective and flexible depletion of problematic sequences from RNA-seq libraries at the cDNA stage", BMC GENOMICS, vol. 15, no. 401, 31 December 2014 (2014-12-31), XP021187323, ISSN: 1471-2164

BEIJING JANLEA PATENT AGENCY CO., LTD. (CN)

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