Title:
METHOD FOR SCREENING ANTIFUNGAL AGENTS
Document Type and Number:
WIPO Patent Application WO/2019/117273
Kind Code:
A1
Abstract:
Provided is a means for developing an antifungal agent that has few adverse effects in humans and employs an entirely novel action mechanism. Also provided is a method for screening an antifungal agent targeting fungal Erg25 or Erg26 as a molecular target. The method is characterized by evaluating an action of a test substance having an antifungal activity on fungal growth under conditions in which the activity of Erg11 is inhibited, and selecting a substance which allows fungal growth.
Inventors:
CHIBANA HIROJI (JP)
SATO MICHIYO (JP)
SATO MICHIYO (JP)
Application Number:
PCT/JP2018/046013
Publication Date:
June 20, 2019
Filing Date:
December 14, 2018
Export Citation:
Assignee:
UNIV CHIBA NAT UNIV CORP (JP)
International Classes:
C12Q1/18; A61K45/00; A61P31/10; C12Q1/527; G01N33/15
Other References:
NOSE, H. ET AL.: "PF1163A, a novel antifungal agent, inhibit ergosterol biosynthesis at C-4 sterol methyl oxidase.", THE JOURNAL OF ANTIBIOTICS, vol. 55, no. 11, November 2002 (2002-11-01), pages 969 - 974, XP008064130
GACHOTTE, D. ET AL.: "A yeast sterol auxotroph (erg25) is rescued by addition of azole antifungals and reduced levels of heme", PROC. NATL. ACAD. SCI. USA, vol. 94, no. 21, 14 October 1997 (1997-10-14), pages 11173 - 11178, XP055617694
BAUDRY, K. ET AL.: "The effect of the erg26-1 mutation on the regulation of lipid metabolism in Saccharomyces cerevisiae", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, no. 16, 20 April 2001 (2001-04-20), pages 12702 - 12711, XP055617695
GACHOTTE, D. ET AL.: "A yeast sterol auxotroph (erg25) is rescued by addition of azole antifungals and reduced levels of heme", PROC. NATL. ACAD. SCI. USA, vol. 94, no. 21, 14 October 1997 (1997-10-14), pages 11173 - 11178, XP055617694
BAUDRY, K. ET AL.: "The effect of the erg26-1 mutation on the regulation of lipid metabolism in Saccharomyces cerevisiae", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, no. 16, 20 April 2001 (2001-04-20), pages 12702 - 12711, XP055617695
Attorney, Agent or Firm:
THE PATENT CORPORATE BODY ARUGA PATENT OFFICE (JP)
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