Title:
METHOD FOR SCREENING FOR INHIBITORS OF PROTEIN/PROTEIN INTERACTION AND CORRESPONDING RIBOZYMES
Document Type and Number:
WIPO Patent Application WO2003022858
Kind Code:
A3
Abstract:
The invention relates to an allosterically-controlled hairpin ribozyme. The invention further relates to a polynucleotide comprising a hairpin ribozyme, whereby the hairpin ribozyme comprises at least one substrate-binding domain A and a domain B. The catalytic activity is dependent on contact of domain A with domain B and a domain C is arranged between domain A and domain B. Said domain C connects domain A and domain B and comprises a nucleic acid binding to a target molecule. Furthermore, the invention relates to a polynucleotide, comprising a hairpin ribozyme containing at least one substrate-binding domain A and a catalytic domain B. The catalytic activity is dependent on contact of domain A with domain B and a domain C is arranged between domain A and domain B. Said domain C connects domain A and domain B and comprises a nucleic acid at least partially complementary to a nucleic acid binding to a target molecule.
Inventors:
NAJAFI-SHOUSHTARI SEYED HANI (DE)
HARTIG JOERG (DE)
FAMULOK MICHAEL (DE)
HARTIG JOERG (DE)
FAMULOK MICHAEL (DE)
Application Number:
PCT/EP2002/010140
Publication Date:
August 28, 2003
Filing Date:
September 10, 2002
Export Citation:
Assignee:
NASCACELL GMBH (DE)
NAJAFI-SHOUSHTARI SEYED HANI (DE)
HARTIG JOERG (DE)
FAMULOK MICHAEL (DE)
NAJAFI-SHOUSHTARI SEYED HANI (DE)
HARTIG JOERG (DE)
FAMULOK MICHAEL (DE)
International Classes:
C12N15/113; A61K38/00; (IPC1-7): C12N9/00; C12N15/11
Domestic Patent References:
| WO2002042491A2 | 2002-05-30 |
Foreign References:
| EP0277096A2 | 1988-08-03 | |||
| EP0468327A2 | 1992-01-29 | |||
| US5900407A | 1999-05-04 | |||
| US4959212A | 1990-09-25 |
Other References:
ESTEBAN ET AL.: "Structural basis for heterogeneous kinetics: Reengineering the hairpin ribozyme", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCE US, vol. 95, May 1998 (1998-05-01), pages 6091 - 6096, XP002234998
WALTER ET AL.: "Stability of hairpin ribozyme tertiary structure is governed by the interdomain junction", NATURE STRUCTURAL BIOLOGY, vol. 6, no. 6, 1999, pages 544 - 549, XP002234999
SOUKUP GARRETT A ET AL: "Engineering precision RNA molecular switches", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 96, no. 7, 30 March 1999 (1999-03-30), pages 3584 - 3589, XP002158536, ISSN: 0027-8424
FAMULOK M: "OLIGONUCLEOTIDE APTAMERS THAT RECOGNIZE SMALL MOLECULES", CURRENT OPINION IN STRUCTURAL BIOLOGY, CURRENT BIOLOGY LTD., LONDON, GB, vol. 9, no. 3, 1999, pages 324 - 329, XP001031183, ISSN: 0959-440X
ROBERTSON MICHAEL P ET AL: "Design and optimization of effector-activated ribozyme ligases", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 28, no. 8, 15 April 2000 (2000-04-15), pages 1751 - 1759, XP002205328, ISSN: 0305-1048
KOMATSU ET AL.: "In vitro selection of hairpin ribozymes activated with short oligonucleotides", BIOCHEMISTRY, vol. 41, 23 July 2002 (2002-07-23), pages 9090 - 9098, XP002235000
WALTER ET AL.: "Stability of hairpin ribozyme tertiary structure is governed by the interdomain junction", NATURE STRUCTURAL BIOLOGY, vol. 6, no. 6, 1999, pages 544 - 549, XP002234999
SOUKUP GARRETT A ET AL: "Engineering precision RNA molecular switches", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 96, no. 7, 30 March 1999 (1999-03-30), pages 3584 - 3589, XP002158536, ISSN: 0027-8424
FAMULOK M: "OLIGONUCLEOTIDE APTAMERS THAT RECOGNIZE SMALL MOLECULES", CURRENT OPINION IN STRUCTURAL BIOLOGY, CURRENT BIOLOGY LTD., LONDON, GB, vol. 9, no. 3, 1999, pages 324 - 329, XP001031183, ISSN: 0959-440X
ROBERTSON MICHAEL P ET AL: "Design and optimization of effector-activated ribozyme ligases", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 28, no. 8, 15 April 2000 (2000-04-15), pages 1751 - 1759, XP002205328, ISSN: 0305-1048
KOMATSU ET AL.: "In vitro selection of hairpin ribozymes activated with short oligonucleotides", BIOCHEMISTRY, vol. 41, 23 July 2002 (2002-07-23), pages 9090 - 9098, XP002235000
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