Title:
METHOD FOR SCREENING NUCLEIC ACID APTAMER
Document Type and Number:
WIPO Patent Application WO/2017/126646
Kind Code:
A1
Abstract:
The present invention pertains to a method for screening a nucleic acid aptamer that includes the following steps: (a) bringing a target molecule fixed to a solid-phase carrier into contact with a nucleic acid aptamer candidate; (b) fractionating the nucleic acid aptamer candidate bound to the target molecule via capillary electrophoresis; and (c) amplifying the nucleic acid aptamer candidate via PCR.
Inventors:
YOSHIMOTO KEITARO (JP)
WAKUI KOJI (JP)
FURUSHO HITOSHI (JP)
WAKUI KOJI (JP)
FURUSHO HITOSHI (JP)
Application Number:
PCT/JP2017/001873
Publication Date:
July 27, 2017
Filing Date:
January 20, 2017
Export Citation:
Assignee:
UNIV TOKYO (JP)
NISSAN CHEMICAL IND LTD (JP)
NISSAN CHEMICAL IND LTD (JP)
International Classes:
C12N15/115
Foreign References:
| JP2016171795A | 2016-09-29 |
Other References:
WAKUI K ET AL.: "Development of in vitro selection method for DNA aptamers based on the combined use of CE-SELEX and magnetic beads", CSJ: CHEMICAL SOCIETY OF JAPAN 96TH CSJ ANNUAL MEETING, vol. 96, 24 March 2016 (2016-03-24), pages Abstr. 1PB-055 - 055, XP009512736
WAKUI K ET AL.: "In vitro selection of DNA aptamer by beads based capillary electrophoresis", 43RD ISNAC, 27 September 2016 (2016-09-27), pages 252 - 253, XP009513844
YANG J ET AL.: "Capillary electrophoresis-SELEX selection of catalytic DNA aptamers for a small- molecule porphyrin target", ANALYTICAL CHEMISTRY, vol. 85, no. 3, 5 February 2013 (2013-02-05), pages 1525 - 1530, XP055507535
MOSING RK ET AL.: "Capillary electrophoresis- SELEX selection of aptamers with affinity for HIV-1 reverse transcriptase", ANALYTICAL CHEMISTRY, vol. 77, 2005, pages 6107 - 6112, XP003020888
YANG, J.; BOWSER, M. T.: "Capillary Electrophoresis-SELEX Selection of Catalytic DNA Aptamers for a Small-Molecule Porphyrin Target", ANAL. CHEM., vol. 85, 2013, pages 1525 - 1530
WAKUI K ET AL.: "In vitro selection of DNA aptamer by beads based capillary electrophoresis", 43RD ISNAC, 27 September 2016 (2016-09-27), pages 252 - 253, XP009513844
YANG J ET AL.: "Capillary electrophoresis-SELEX selection of catalytic DNA aptamers for a small- molecule porphyrin target", ANALYTICAL CHEMISTRY, vol. 85, no. 3, 5 February 2013 (2013-02-05), pages 1525 - 1530, XP055507535
MOSING RK ET AL.: "Capillary electrophoresis- SELEX selection of aptamers with affinity for HIV-1 reverse transcriptase", ANALYTICAL CHEMISTRY, vol. 77, 2005, pages 6107 - 6112, XP003020888
YANG, J.; BOWSER, M. T.: "Capillary Electrophoresis-SELEX Selection of Catalytic DNA Aptamers for a Small-Molecule Porphyrin Target", ANAL. CHEM., vol. 85, 2013, pages 1525 - 1530
Attorney, Agent or Firm:
TSUKUNI & ASSOCIATES et al. (JP)
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