Title:
METHOD FOR THE SELECTION AND IDENTIFICATION OF PEPTIDE OR PROTEIN MOLECULES BY MEANS OF PHASE DISPLAY
Document Type and Number:
WIPO Patent Application WO2003046198
Kind Code:
A3
Abstract:
The invention relates to a method for the selection and identification of at least one representative (interaction partner) from a plurality of peptide or protein molecules, which can specifically interact with at least one representative from a plurality of target molecules, forming a bond. The inventive method comprises the following steps: (a) a virus system consisting of a plurality of viruses, wherein each virus respectively presents at least one representative from the plurality of peptide or protein molecules on the surface thereof, is brought into contact with the plurality of target molecules (ligands) which are immobilized on the surface of a solid phase carrier such that they are position addressable in a two-dimensional grid; (b) unbound viruses are removed from the surface thereof; and (c) the interaction partner is identified by detection and determination of the position of the bond between the immobilized ligand and the interaction partner presented by the virus with the aid of a marker-free detection method. The described method makes it possible to concentrate viruses presenting interaction partners by means of an optionally cyclic repetition of selection. Optionally, selected interaction partners are recombinantly expressed after identification of the coding nucleotide sequence. .
Inventors:
HILL OLIVER (DE)
OTTLEBEN HOLGER (DE)
DICKOPF STEFAN (DE)
BURKERT KLAUS (DE)
OTTLEBEN HOLGER (DE)
DICKOPF STEFAN (DE)
BURKERT KLAUS (DE)
Application Number:
PCT/EP2002/013010
Publication Date:
March 25, 2004
Filing Date:
November 20, 2002
Export Citation:
Assignee:
GRAFFINITY PHARMACEUTICALS AG (DE)
HILL OLIVER (DE)
OTTLEBEN HOLGER (DE)
DICKOPF STEFAN (DE)
BURKERT KLAUS (DE)
HILL OLIVER (DE)
OTTLEBEN HOLGER (DE)
DICKOPF STEFAN (DE)
BURKERT KLAUS (DE)
International Classes:
G01N21/55; B01L3/00; (IPC1-7): C12N15/10; C12Q1/68; G01N21/55; G01N21/75; G01N33/68
Domestic Patent References:
| WO2001009606A1 | 2001-02-08 | |||
| WO2002025630A2 | 2002-03-28 | |||
| WO2000073796A2 | 2000-12-07 | |||
| WO1995022754A1 | 1995-08-24 | |||
| WO2001002554A2 | 2001-01-11 | |||
| WO2001079559A1 | 2001-10-25 |
Foreign References:
| US20010026943A1 | 2001-10-04 | |||
| US5955729A | 1999-09-21 |
Other References:
STOOP A A ET AL: "High-density mutagenesis by combined DNA shuffling and phage display to assign essential amino acid residues in protein-protein interactions: application to study structure-function of plasminogen activation inhibitor 1 (PAI-I)", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 301, no. 5, 1 September 2000 (2000-09-01), pages 1135 - 1147, XP004461687, ISSN: 0022-2836
FREUNDLIEB S, GAMER J: "Chemical Microarrays: A Novel Approach to Drug Discovery", NEW DRUGS, no. 3, 2001, pages 54 - 60, XP002259718
HOOGENBOOM H ET AL: "Antibody phage display technology and its applications", IMMUNOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS BV, NL, vol. 4, no. 4, 1998, pages 1 - 20, XP002105422, ISSN: 1380-2933
"T7Select System Manual", NOVAGEN PRODUCT DOCUMENTS AND TECHNICAL LITERATURE, June 2000 (2000-06-01), pages 1 - 21, XP002259677, Retrieved from the Internet [retrieved on 20031029]
FREUNDLIEB S, GAMER J: "Chemical Microarrays: A Novel Approach to Drug Discovery", NEW DRUGS, no. 3, 2001, pages 54 - 60, XP002259718
HOOGENBOOM H ET AL: "Antibody phage display technology and its applications", IMMUNOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS BV, NL, vol. 4, no. 4, 1998, pages 1 - 20, XP002105422, ISSN: 1380-2933
"T7Select System Manual", NOVAGEN PRODUCT DOCUMENTS AND TECHNICAL LITERATURE, June 2000 (2000-06-01), pages 1 - 21, XP002259677, Retrieved from the Internet
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