METHOD FOR SEPARATING AND COLLECTING CELLS FROM BIOLOGICAL TISSUE

WIPO Patent Application WO/2019/059278

A1

Provided is a method for enzymatically treating a biological tissue under low-load conditions for cells to separate and collect cells. The present invention relates to a method for separating and collecting cells from a biological tissue, the method including: step 1 of mixing the biological tissue with an enzyme solution in a volume of V1 in a container to perform an enzymatic treatment, thereby preparing an enzymatically treated solution containing free cells; step 2 of allowing the container to stand to cause the separation of the enzymatically treated solution into an oily layer and an aqueous layer until a point of time at which the volume of the aqueous layer does not exceed 0.93 V1; step 3 of collecting the aqueous layer from the container and causing the oily layer to remain in the container at the point of time mentioned in step 2; and step 4 of supplying a washing solution into the container, then mixing the resultant solution with the oily layer remaining in the container, then causing the resultant mixture to stand to cause the separation into an oily layer and an aqueous layer, and then collecting the aqueous layer from the container and causing the oily layer to remain in the container.

HAYASHI SHINJI (JP)

PCT/JP2018/034815

March 28, 2019

September 20, 2018

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KANEKA CORP (JP)

C12N5/077

WO2011111386A1

2011-09-15

JP2007289076A	2007-11-08
JP2007185157A	2007-07-26
JP2011010583A	2011-01-20
JP2015519067A	2015-07-09

NAKAYAMA, TAKAYUKI ET AL.: "CELL THERAPY USING ADIPOSE-DERIVED MESENCHYMAL STROMAL CELLS : CURRENT STATUS AND PERSPECTIVES", JAPANESE JOURNAL OF TRANSFUSION AND CELL THERAPY, vol. 59, no. 3, 2013, pages 450 - 456, XP055586411

HIRAKI & ASSOCIATES (JP)

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