Title:
METHOD AND SPECIMEN FOR DETECTING SENSITIVITY TO ISONIAZID IN TUBERCLE BACILLUS
Document Type and Number:
WIPO Patent Application WO/2010/001924
Kind Code:
A1
Abstract:
Disclosed is a novel means for detecting the sensitivity to isoniazid (INH) in a tubercle bacillus, i.e., for determining whether or not a tubercle bacillus is an INH-resistant bacterium, more reliably. Specifically disclosed is a method for detecting the sensitivity to isoniazid in a tubercle bacillus, which comprises the steps of: obtaining a fabG1 gene of the tubercle bacillus in a sample; and detecting the occurrence of a mutation associated with the resistance to isoniazid in the fabG1 gene. If required, the method may further include a step of detecting the occurrence of a mutation in a furA gene of the tubercle bacillus. Preferably, the method is combined with another known isoniazid resistance detection method.
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Inventors:
KIRIKAE TERUO (JP)
ANDOU HIROKI (JP)
SUETAKE TOSHINORI (JP)
NAKAMURA TOMOHIKO (JP)
ANDOU HIROKI (JP)
SUETAKE TOSHINORI (JP)
NAKAMURA TOMOHIKO (JP)
Application Number:
PCT/JP2009/062034
Publication Date:
January 07, 2010
Filing Date:
July 01, 2009
Export Citation:
Assignee:
NIPRO CORP (JP)
JAPAN GOVERNMENT (JP)
KIRIKAE TERUO (JP)
ANDOU HIROKI (JP)
SUETAKE TOSHINORI (JP)
NAKAMURA TOMOHIKO (JP)
JAPAN GOVERNMENT (JP)
KIRIKAE TERUO (JP)
ANDOU HIROKI (JP)
SUETAKE TOSHINORI (JP)
NAKAMURA TOMOHIKO (JP)
International Classes:
C12Q1/68; C12N15/09; G01N33/53; G01N37/00
Foreign References:
| JP2004215542A | 2004-08-05 | |||
| JP2001103981A | 2001-04-17 | |||
| JP3579049B2 | 2004-10-20 | |||
| JPH09501823A | 1997-02-25 | |||
| JPH0681600B2 | 1994-10-19 |
Other References:
RAMASWAMY, S., V. ET AL.: "Single Nucleotide Polymorphisms in Genes Associated with Isoniazid Resistance in Mycobacterium tuberculosis", ANTIMICROB. AGENTS CHEMOTHER., vol. 47, no. 4, 2003, pages 1241 - 1250, XP008139084
ZHANG, M. ET AL.: "Detection of Mutations Associated with Isoniazid Resistance in Mycobacterium tuberculosis Isolates from China", J. CLIN. MICROBIOL., vol. 43, no. 11, 2005, pages 5477 - 5482, XP008139011
SEKIGUCHI, J. ET AL.: "Detection of Multidrug Resistance in Mycobacterium tuberculosis", J. CLIN. MICROBIOL., vol. 45, no. 1, 2007, pages 179 - 192, XP008139017
DOUSTDAR, F. ET AL.: "Molecular Analysis of Isoniazid Resistance in Different Genotypes of Mycobacterium tuberculosis Isolates from Iran", MICROB. DRUG RESIST., vol. 14, no. 4, December 2008 (2008-12-01), pages 273 - 279, XP008139086
See also references of EP 2322659A4
PARISH ET AL., J. BACT., vol. 189, no. 10, 2007, pages 3721 - 3728
COHEN-GONSAUD ET AL., J. MOL. BIOL., vol. 320, no. 2, 2002, pages 249 - 261
MARRAKCHI ET AL., MICROBIOLOGY, vol. 148, 2002, pages 951 - 960
A.S. PYM ET AL., MOLECULAR MICROBIOLOGY, vol. 40, 2001, pages 879 - 889
NOLLAU ET AL., CLIN. CHEM., vol. 43, 1997, pages 1114 - 1120
LANDEGREN, U. ET AL.: "Laboratory Protocols for Mutation Detection", 1996, OXFORD UNIVERSITY PRESS
NEWTON ET AL.: "PCR", 1997, BIOS SCIENTIFIC PUBLISHERS LIMITED
ORITA, M. ET AL., PROC. NATL. ACAD. SCI. USA., vol. 86, no. 8, 1989, pages 2766 - 2770
SHEFFIELD, V. C. ET AL., PROC. NATL. ACAD. SCI. USA., vol. 86, no. 1, 1989, pages 232 - 236
LIVAK, K. J., GENEL. ANAL., vol. 14, 1999, pages 143
MORRIS, T. ET AL., J. CLIN. MICROBIOL., vol. 34, 1996, pages 2933
GRIFFIN, T. J. ET AL., PROC. NATL. ACAD. SCI. USA., vol. 96, no. 11, 1999, pages 6301 - 6
MURRAY, J. C. ET AL., PROC. NATL. ACAD. SCI. USA., vol. 80, no. 19, 1983, pages 5951 - 5955
KOKORIS, K. ET AL., J. MED. GENET., vol. 36, 1999, pages 730
ZHANG, M. ET AL.: "Detection of Mutations Associated with Isoniazid Resistance in Mycobacterium tuberculosis Isolates from China", J. CLIN. MICROBIOL., vol. 43, no. 11, 2005, pages 5477 - 5482, XP008139011
SEKIGUCHI, J. ET AL.: "Detection of Multidrug Resistance in Mycobacterium tuberculosis", J. CLIN. MICROBIOL., vol. 45, no. 1, 2007, pages 179 - 192, XP008139017
DOUSTDAR, F. ET AL.: "Molecular Analysis of Isoniazid Resistance in Different Genotypes of Mycobacterium tuberculosis Isolates from Iran", MICROB. DRUG RESIST., vol. 14, no. 4, December 2008 (2008-12-01), pages 273 - 279, XP008139086
See also references of EP 2322659A4
PARISH ET AL., J. BACT., vol. 189, no. 10, 2007, pages 3721 - 3728
COHEN-GONSAUD ET AL., J. MOL. BIOL., vol. 320, no. 2, 2002, pages 249 - 261
MARRAKCHI ET AL., MICROBIOLOGY, vol. 148, 2002, pages 951 - 960
A.S. PYM ET AL., MOLECULAR MICROBIOLOGY, vol. 40, 2001, pages 879 - 889
NOLLAU ET AL., CLIN. CHEM., vol. 43, 1997, pages 1114 - 1120
LANDEGREN, U. ET AL.: "Laboratory Protocols for Mutation Detection", 1996, OXFORD UNIVERSITY PRESS
NEWTON ET AL.: "PCR", 1997, BIOS SCIENTIFIC PUBLISHERS LIMITED
ORITA, M. ET AL., PROC. NATL. ACAD. SCI. USA., vol. 86, no. 8, 1989, pages 2766 - 2770
SHEFFIELD, V. C. ET AL., PROC. NATL. ACAD. SCI. USA., vol. 86, no. 1, 1989, pages 232 - 236
LIVAK, K. J., GENEL. ANAL., vol. 14, 1999, pages 143
MORRIS, T. ET AL., J. CLIN. MICROBIOL., vol. 34, 1996, pages 2933
GRIFFIN, T. J. ET AL., PROC. NATL. ACAD. SCI. USA., vol. 96, no. 11, 1999, pages 6301 - 6
MURRAY, J. C. ET AL., PROC. NATL. ACAD. SCI. USA., vol. 80, no. 19, 1983, pages 5951 - 5955
KOKORIS, K. ET AL., J. MED. GENET., vol. 36, 1999, pages 730
Attorney, Agent or Firm:
NANJO, Hiromichi (JP)
Hiromichi Nanjo (JP)
Hiromichi Nanjo (JP)
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