Title:
METHOD OF SUBCULTURING PLURIPOTENT STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2014/115799
Kind Code:
A1
Abstract:
[Problem] The purpose of the present invention is to provide a method of simply and uniformly subculturing pluripotent stem cells. [Solution] When passaging pluripotent stem cells, this method of subculturing pluripotent stem cells involves dispersing to the level of single cells cell clumps obtained by culturing, and thereafter, quickly forming cell aggregates.
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Inventors:
OZAKI SHIGENORI (JP)
GOMI SHINICHI (JP)
KURAKAZU TOMOAKI (JP)
OSHIMA YASUHIRO (JP)
KAWAMATA SHIN (JP)
NISHISHITA NAOKI (JP)
GOMI SHINICHI (JP)
KURAKAZU TOMOAKI (JP)
OSHIMA YASUHIRO (JP)
KAWAMATA SHIN (JP)
NISHISHITA NAOKI (JP)
Application Number:
PCT/JP2014/051362
Publication Date:
July 31, 2014
Filing Date:
January 23, 2014
Export Citation:
Assignee:
TOKYO ELECTRON LTD (JP)
FOUND BIOMEDICAL RES & INNOV (JP)
FOUND BIOMEDICAL RES & INNOV (JP)
International Classes:
C12N5/07; C12M1/18; C12N5/071
Domestic Patent References:
| WO2007114351A1 | 2007-10-11 | |||
| WO2011022507A1 | 2011-02-24 | |||
| WO2011068879A2 | 2011-06-09 | |||
| WO2012087965A2 | 2012-06-28 | |||
| WO2011161962A1 | 2011-12-29 |
Foreign References:
| JP2010233456A | 2010-10-21 | |||
| JP2002000178A | 2002-01-08 |
Other References:
KANJI YAHIRO ET AL.: "Introduction of stem cell culture, differentiation and assay tools", THE CELL, vol. 44, no. 9, 20 August 2012 (2012-08-20), pages 393 - 397, XP008179927
STOVER, A.E. ET AL.: "The Generation of Embryoid Bodies from Feeder-Based or Feeder- Free Human Pluripotent Stem Cell Cultures", METHODS IN MOLECULAR BIOLOGY, vol. 767, 2011, pages 391 - 398, XP055265964
"AGGREWELL: ARE YOU MAKING EMBRYOID BODIES? (STEMCELL TECHNOLOGIES)", 25 September 2009 (2009-09-25), XP054976485, Retrieved from the Internet [retrieved on 20140409]
HIROMICH YOSHIOKA ET AL.: "iPS Saibo Yurai Bunka Saibo to Mibunka iPS Saibo no Isosa ni yoru Hi Shinshuteki Shikibetsu", DAI 64 KAI ABSTRACTS OF THE ANNUAL MEETING OF THE SOCIETY FOR BIOTECHNOLOGY, 25 September 2012 (2012-09-25), JAPAN, pages 112, XP008179912
WATANABE, K.: "A ROCK inhibitor permits survival of dissociated human embryonic stem cells", NATURE BBOTECHNOLOGY, vol. 25, 2007, pages 681, XP002478043, DOI: doi:10.1038/nbt1310
SPELKE D.P.: "Methods for embryoid body formation: the microwell approach", METHODS IN MOLECULAR BIOLOGY, vol. 690, 2011, pages 151 - 162
SHIMAZAKI TAKUYA; OKADA YOHEI; YOSIJAKI DHAKAHITO; OKANO HIDEYUKI: "Protein, Nucleic acid and Enzyme", KIORITZ PUBLICATION, vol. 51, no. 13, 2006, pages 1854 - 1861
See also references of EP 2949746A4
STOVER, A.E. ET AL.: "The Generation of Embryoid Bodies from Feeder-Based or Feeder- Free Human Pluripotent Stem Cell Cultures", METHODS IN MOLECULAR BIOLOGY, vol. 767, 2011, pages 391 - 398, XP055265964
"AGGREWELL: ARE YOU MAKING EMBRYOID BODIES? (STEMCELL TECHNOLOGIES)", 25 September 2009 (2009-09-25), XP054976485, Retrieved from the Internet
HIROMICH YOSHIOKA ET AL.: "iPS Saibo Yurai Bunka Saibo to Mibunka iPS Saibo no Isosa ni yoru Hi Shinshuteki Shikibetsu", DAI 64 KAI ABSTRACTS OF THE ANNUAL MEETING OF THE SOCIETY FOR BIOTECHNOLOGY, 25 September 2012 (2012-09-25), JAPAN, pages 112, XP008179912
WATANABE, K.: "A ROCK inhibitor permits survival of dissociated human embryonic stem cells", NATURE BBOTECHNOLOGY, vol. 25, 2007, pages 681, XP002478043, DOI: doi:10.1038/nbt1310
SPELKE D.P.: "Methods for embryoid body formation: the microwell approach", METHODS IN MOLECULAR BIOLOGY, vol. 690, 2011, pages 151 - 162
SHIMAZAKI TAKUYA; OKADA YOHEI; YOSIJAKI DHAKAHITO; OKANO HIDEYUKI: "Protein, Nucleic acid and Enzyme", KIORITZ PUBLICATION, vol. 51, no. 13, 2006, pages 1854 - 1861
See also references of EP 2949746A4
Attorney, Agent or Firm:
KATSUNUMA Hirohito et al. (JP)
Katsunuma Hirohito (JP)
Katsunuma Hirohito (JP)
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