Title:
METHOD FOR TESTING FOR COLON TUMORS
Document Type and Number:
WIPO Patent Application WO/2017/043497
Kind Code:
A1
Abstract:
The presence of colon tumors in a subject is predicted, without performing a bisulfite treatment, by providing in the following order: (a) a step for extracting the DNA in a biological sample collected from a subject; (b) a step for treating the DNA obtained in step (a) using a methylation-sensitive restriction enzyme; (c) a step for amplifying a region encoding the TWIST1 gene, BMP3 gene, NDRG4 gene, or SEPT9 gene, a region from -1 to -1100 from a start codon of any one of these genes, or part of a region from -300 to -500 from a transcription start point of any one of these genes in the DNA treated using the methylation-sensitive restriction enzyme in step (b), and measuring the degree of methylation of one or more CpG sequences within the amplified DNA; and (d) a step for predicting the presence of colon tumors in the subject when the degree of methylation measured in step (c) is at or above a prescribed value, and predicting the absence of colon tumors in the subject when the degree of methylation is less than a prescribed value.
Inventors:
SUEHIRO YUTAKA (JP)
Application Number:
PCT/JP2016/076218
Publication Date:
March 16, 2017
Filing Date:
September 07, 2016
Export Citation:
Assignee:
UNIV YAMAGUCHI (JP)
International Classes:
C12Q1/68; C12M1/34; C12N15/09
Domestic Patent References:
| WO2010113529A1 | 2010-10-07 | |||
| WO2015029947A1 | 2015-03-05 | |||
| WO2014073785A1 | 2014-05-15 |
Foreign References:
| JP2014520520A | 2014-08-25 | |||
| JP2007244377A | 2007-09-27 | |||
| JP2013074837A | 2013-04-25 |
Other References:
PEREZ-CARBONELL, L. ET AL.: "IGFBP3 methylation is a novel diagnostic and predictive biomarker in colorectal cancer", PLOS ONE, vol. 9, no. 8, 2014, pages 1 - 11
ZOU, H. ET AL.: "Quantification of methylated markers with a multiplex methylation-specific technology", CLINICAL CHEMISTRY, vol. 58, no. 2, 2012, pages 375 - 383, XP055105588
MITCHELL, S. M. ET AL.: "A panel of genes methylated with high frequency in colorectal cancer", BMC CANCER, vol. 14, no. 45, 2014, pages 1 - 15, XP021175595
ZOU, H. ET AL.: "Quantification of methylated markers with a multiplex methylation-specific technology", CLINICAL CHEMISTRY, vol. 58, no. 2, 2012, pages 375 - 383, XP055105588
MITCHELL, S. M. ET AL.: "A panel of genes methylated with high frequency in colorectal cancer", BMC CANCER, vol. 14, no. 45, 2014, pages 1 - 15, XP021175595
Attorney, Agent or Firm:
FUJIMOTO, Shohei (JP)
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