Title:
METHOD FOR USING A BIOREACTOR WITH DUAL COMPARTMENT
Document Type and Number:
WIPO Patent Application WO/2009/153425
Kind Code:
A3
Abstract:
The invention relates to a method for using a dual-compartment bioreactor that comprises a flexible pouch (1) with two compartments (2, 3) separated by a filter (4), as well as at least one inlet opening (5) in communication with one of the compartments (2) and at least one outlet opening (6) in communication with the other compartment (3), wherein said method comprises the implementation thereof in biological processing equipment particularly for washing or converting a tissue or biological product, characterised in that the method comprises, for a tissue dissociation, using said bioreactor as a digestion chamber and manually kneading the flexible pouch (1) for dissociating the tissues during a digestion step in which a digestion solution containing collagenase flows between the inlet (5) and outlet (6) openings of the pouch.
Inventors:
BENBUNAN MARC (FR)
CATTAN PIERRE (FR)
LARGHERO JEROME (FR)
CATTAN PIERRE (FR)
LARGHERO JEROME (FR)
Application Number:
PCT/FR2009/000550
Publication Date:
September 30, 2010
Filing Date:
May 13, 2009
Export Citation:
Assignee:
ASSIST PUBL HOPITAUX DE PARIS (FR)
BENBUNAN MARC (FR)
CATTAN PIERRE (FR)
LARGHERO JEROME (FR)
BENBUNAN MARC (FR)
CATTAN PIERRE (FR)
LARGHERO JEROME (FR)
International Classes:
C12M3/08
Domestic Patent References:
| WO2004111181A1 | 2004-12-23 | |||
| WO1996030497A1 | 1996-10-03 |
Foreign References:
| FR2797887A1 | 2001-03-02 | |||
| EP0725134A2 | 1996-08-07 |
Attorney, Agent or Firm:
CABINET ORES et al. (Paris, Paris, FR)
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