Title:
METHOD FOR VERIFYING THE PRIMARY STRUCTURE OF PROTEIN
Document Type and Number:
WIPO Patent Application WO/2017/088769
Kind Code:
A1
Abstract:
Disclosed herein is a method for verifying the primary structure of a protein through comparative analyses between ion clusters observed in mass spectra and a series of simulated ion clusters deduced from its putative chemical formula. The method comprises the steps of: preparing a protein sample for mass spectrometric analyses; collecting mass spectra of the protein sample; obtaining master ion cluster from a plurality of ion clusters in the mass spectra; producing a series of simulated ion clusters according to the chemical formula of the protein; finding the best fit for the master ion cluster among the series of simulated ion clusters; and verifying if said best-fit simulated ion cluster corresponds to the chemical formula of the protein.
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Inventors:
TSAY YEOU-GUANG (CN)
CHEN YA-FEN (CN)
CHEN YA-FEN (CN)
Application Number:
PCT/CN2016/106962
Publication Date:
June 01, 2017
Filing Date:
November 23, 2016
Export Citation:
Assignee:
SUN JET BIOTECHNOLOGY INC (CN)
International Classes:
G01N33/68; G16B15/00; G16B40/10
Foreign References:
| US20120261568A1 | 2012-10-18 | |||
| US20110282587A1 | 2011-11-17 | |||
| US8502137B2 | 2013-08-06 | |||
| CN101115991A | 2008-01-30 |
Other References:
CHEN, YAFEN ET AL.: "Determination of accurate protein monoisotopic mass with the most abundant mass measurable using high-resolution mass spectrometry", ANALYTICAL BIOCHEMISTRY, vol. 440, 4 June 2013 (2013-06-04), pages 108 - 113, XP028676657
LIU, NAIYU ET AL.: "Examination of segmental average mass spectra from liquid chromatographyetandem mass spectrometric (LCeMS/MS) data enables screening of multiple types of protein modifications", ANALYTICA CHIMICA ACTA, vol. 892, 10 August 2015 (2015-08-10), pages 115 - 122, XP029275893
DAISUKE, M. ET AL.: "A Strategy for the Determination of the Elemental Composition by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Based on Isotopic Peak Ratios", ANALYTICAL CHEMISTRY, vol. 82, no. 13, 3 June 2010 (2010-06-03), pages 5887 - 5891, XP055385666
See also references of EP 3380844A4
LIU, NAIYU ET AL.: "Examination of segmental average mass spectra from liquid chromatographyetandem mass spectrometric (LCeMS/MS) data enables screening of multiple types of protein modifications", ANALYTICA CHIMICA ACTA, vol. 892, 10 August 2015 (2015-08-10), pages 115 - 122, XP029275893
DAISUKE, M. ET AL.: "A Strategy for the Determination of the Elemental Composition by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Based on Isotopic Peak Ratios", ANALYTICAL CHEMISTRY, vol. 82, no. 13, 3 June 2010 (2010-06-03), pages 5887 - 5891, XP055385666
See also references of EP 3380844A4
Attorney, Agent or Firm:
GE CHENG & CO.,LTD. (CN)
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