METHODS AND COMPOSITIONS FOR DISCRIMINATION BETWEEN CYTOSINE AND MODIFICATIONS THEREOF, AND FOR METHYLOME ANALYSIS

Title:

METHODS AND COMPOSITIONS FOR DISCRIMINATION BETWEEN CYTOSINE AND MODIFICATIONS THEREOF, AND FOR METHYLOME ANALYSIS

Document Type and Number:

WIPO Patent Application WO/2013/138644

Kind Code:

A3

Abstract:

Compositions and methods are provided for discrimination between cytosine and modifications thereof using cytidine deaminases and/or oxygenases. Variants of wild type cytidine deaminases are described which show reduced bias with respect to adjacent nucleotides upstream of the cytosine. The methods provide a rapid and convenient use of enzymes to obtain methylomes.

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Inventors:

VAISVILA ROMUALDAS (US)
JOHNSON HEIDI ERIKA (US)
VAINAUSKAS SAULIUS (US)
DAVIS THEODORE B (US)

Application Number:

PCT/US2013/031620

Publication Date:

November 07, 2013

Filing Date:

March 14, 2013

Export Citation:

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Assignee:

NEW ENGLAND BIOLABS INC (US)
VAISVILA ROMUALDAS (US)
JOHNSON HEIDI ERIKA (US)
VAINAUSKAS SAULIUS (US)

International Classes:

C12N9/78

Domestic Patent References:

WO2005005660A1

2005-01-20

Foreign References:

US20060210990A1

2006-09-21

Other References:

S. M. KINNEY ET AL: "Tissue-specific Distribution and Dynamic Changes of 5-Hydroxymethylcytosine in Mammalian Genomes", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 286, no. 28, 15 July 2011 (2011-07-15), pages 24685 - 24693, XP055057583, ISSN: 0021-9258, DOI: 10.1074/jbc.M110.217083
MIAO YU ET AL: "Base-Resolution Analysis of 5-Hydroxymethylcytosine in the Mammalian Genome", CELL, vol. 149, no. 6, 1 June 2012 (2012-06-01), pages 1368 - 1380, XP055064960, ISSN: 0092-8674, DOI: 10.1016/j.cell.2012.04.027

Attorney, Agent or Firm:

STRIMPEL, Harriet, M. (Inc.240 County Roa, Ipswich MA, US)

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