Title:
METHODS AND COMPOSITIONS FOR PURIFICATION OF TRIMERIC FUSION PROTEINS
Document Type and Number:
WIPO Patent Application WO/2022/042716
Kind Code:
A1
Abstract:
The present disclosure provides methods and compositions for affinity purification of proteins containing a C-terminal polypeptide sequence of a procollagen, such as trimeric fusion proteins. Also provided are polypeptides, fusion proteins, and recombinant polypeptides containing an Endo180 ecto-domain or portion thereof capable of reversibly binding a C-terminal polypeptide sequence of a procollagen. Also provided are articles of manufacture, kits, and apparatus related to the Endo180 ecto-domain polypeptides.
Inventors:
LIANG PENG (CN)
Application Number:
PCT/CN2021/115245
Publication Date:
March 03, 2022
Filing Date:
August 30, 2021
Export Citation:
Assignee:
SICHUAN CLOVER BIOPHARMACEUTICALS INC (CN)
International Classes:
C07K1/22; C07K14/705; C07K19/00; C12N5/10; C12N15/12; C12N15/63
Foreign References:
| CN104080480A | 2014-10-01 | |||
| CN103172746A | 2013-06-26 |
Other References:
LIANG JOSHUA G., SU DANMEI, SONG TIAN-ZHANG, ZENG YILAN, HUANG WEIJIN, WU JINHUA, XU RONG, LUO PEIWEN, YANG XIAOFANG, ZHANG XIAODO: "S-Trimer, a COVID-19 subunit vaccine candidate, induces protective immunity in nonhuman primates", NATURE COMMUNICATIONS, vol. 12, no. 1, 1 December 2021 (2021-12-01), XP055873177, DOI: 10.1038/s41467-021-21634-1
ZHAO PEI， ET AL.: "In vitro Interaction of the Cysteine-rich Domain of Endo180 with the C-terminal Region of Type I Collagen", CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 31, no. 5, 31 May 2015 (2015-05-31), pages 521 - 526, XP055902963
THOMAS EMILY K., NAKAMURA MISA, WIENKE DIRK, ISACKE CLARE M., POZZI AMBRA, LIANG PENG: "Endo180 Binds to the C-terminal Region of Type I Collagen", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 280, no. 24, 6 April 2005 (2005-04-06), US , pages 22596 - 22605, XP055902967, ISSN: 0021-9258, DOI: 10.1074/jbc.M501155200
ZHAO PEI， ET AL.: "In vitro Interaction of the Cysteine-rich Domain of Endo180 with the C-terminal Region of Type I Collagen", CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 31, no. 5, 31 May 2015 (2015-05-31), pages 521 - 526, XP055902963
THOMAS EMILY K., NAKAMURA MISA, WIENKE DIRK, ISACKE CLARE M., POZZI AMBRA, LIANG PENG: "Endo180 Binds to the C-terminal Region of Type I Collagen", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 280, no. 24, 6 April 2005 (2005-04-06), US , pages 22596 - 22605, XP055902967, ISSN: 0021-9258, DOI: 10.1074/jbc.M501155200
Attorney, Agent or Firm:
CHENG & PENG INTELLECTUAL PROPERTY LAW OFFICE (CN)
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