Title:
METHODS FOR ISOLATING NUCLEIC ACIDS FROM BIOLOGICAL AND CELLULAR MATERIALS
Document Type and Number:
WIPO Patent Application WO2006036243
Kind Code:
A8
Abstract:
Methods of isolating nucleic acids from samples of biological or cellular material are disclosed which use solid phase binding materials and which avoid the use of any lysis solution or coating. The use of the solid phase binding materials unexpectedly allow the nucleic acid content of cells to be freed and captured directly and in one step. the new methods represent a significant simplification over existing methods. Nucleic acids can be captured and released in a form suitable for downstream processing in under five minutes. Preferred solid phase materials for use with the methods and compositions of the invention comprise a quaternary onium nucleic acid binding portion.
Inventors:
AKHAVAN-TAFTI HASHEM (US)
Application Number:
PCT/US2005/023519
Publication Date:
December 13, 2007
Filing Date:
July 05, 2005
Export Citation:
Assignee:
LUMIGEN INC (US)
AKHAVAN-TAFTI HASHEM (US)
AKHAVAN-TAFTI HASHEM (US)
International Classes:
C12Q1/68; C07H21/00; C07H21/04; C12P19/34
Download PDF:
Previous Patent: NITRIDED BIPOLAR PLATES
Next Patent: ISO/NESTED CASCADING TRIM CONTROL WITH MODEL FEEDBACK UPDATES
Next Patent: ISO/NESTED CASCADING TRIM CONTROL WITH MODEL FEEDBACK UPDATES