Title:
MIX-MODE ANTIBODY AFFINITY SEPARATION MATRIX AND PURIFICATION METHOD USING SAME, AND TARGET MOLECULE
Document Type and Number:
WIPO Patent Application WO/2014/034457
Kind Code:
A1
Abstract:
A mix-mode antibody affinity separation matrix has such a structure that an antibody affinity ligand and a cation exchange group are arranged in a single separation matrix. In a first chromatography step in a process for purifying an antibody or an Fc-containing target molecule, the matrix can improve the purity of the antibody which is the main target substance of the affinity purification, can also improve the selective separation properties of monomers, and can reduce the burden on a subsequent impurity removal step with respect to the removal of aggregates.
Inventors:
MINAKUCHI KAZUNOBU (JP)
Application Number:
PCT/JP2013/072065
Publication Date:
March 06, 2014
Filing Date:
August 19, 2013
Export Citation:
Assignee:
KANEKA CORP (JP)
International Classes:
C07K1/16; C07K1/22; C07K16/00
Domestic Patent References:
| WO2010071208A1 | 2010-06-24 | |||
| WO2011081898A1 | 2011-07-07 | |||
| WO2008085988A1 | 2008-07-17 | |||
| WO2010019493A1 | 2010-02-18 | |||
| WO2010141039A1 | 2010-12-09 | |||
| WO2004074471A1 | 2004-09-02 | |||
| WO2011118699A1 | 2011-09-29 |
Foreign References:
| JP2010517942A | 2010-05-27 | |||
| JP2008505851A | 2008-02-28 | |||
| JP2011517462A | 2011-06-09 | |||
| JP4391830B2 | 2009-12-24 | |||
| JP2010507583A | 2010-03-11 | |||
| US5874165A | 1999-02-23 | |||
| US3932557A | 1976-01-13 | |||
| US4772653A | 1988-09-20 | |||
| US4210723A | 1980-07-01 | |||
| US5250613A | 1993-10-05 | |||
| EP1352957A1 | 2003-10-15 | |||
| US6399750B1 | 2002-06-04 | |||
| US5260373A | 1993-11-09 | |||
| JP2010133733A | 2010-06-17 | |||
| JP2010133734A | 2010-06-17 | |||
| JP2011256176A | 2011-12-22 |
Other References:
GAGNON, P. ET AL.: "Cooperative multimodal retention of IgG, fragments, and aggregates on hydroxyapatite.", JOURNAL OF SEPARATION SCIENCE, vol. 32, no. 22, November 2009 (2009-11-01), pages 3857 - 3865, XP055235544, DOI: doi:10.1002/jssc.200900055
GAGNON, P. ET AL.: "Technology trends in antibody purification.", JOURNAL OF CHROMATOGRAPHY A, vol. 1221, 20 January 2012 (2012-01-20), pages 57 - 70
LACKMANN, M. ET AL.: "Purification and structural analysis of a murine chemotactic cytokine (CP-10) with sequence homology to S100 proteins.", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 267, 15 April 1992 (1992-04-15), pages 7499 - 7504, XP000652185
See also references of EP 2894159A4
HOBER S. ET AL., J. CHROMATOGR. B, vol. 848, 2007, pages 40 - 47
LOW D. ET AL., J. CHROMATOGR. B, vol. 848, 2007, pages 48 - 63
ROQUE A. C. A. ET AL., J. CHROMATOGR. A, vol. 1160, 2007, pages 44 - 55
HERMANSON G.T. ET AL.: "Immobilized Affinity Ligand Techniques", 1992, ACADEMIC PRESS
LJUNGQUIST C. ET AL., EUR. J. BIOCHEM., vol. 186, 1989, pages 557 - 561
GAGNON, P. ET AL.: "Technology trends in antibody purification.", JOURNAL OF CHROMATOGRAPHY A, vol. 1221, 20 January 2012 (2012-01-20), pages 57 - 70
LACKMANN, M. ET AL.: "Purification and structural analysis of a murine chemotactic cytokine (CP-10) with sequence homology to S100 proteins.", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 267, 15 April 1992 (1992-04-15), pages 7499 - 7504, XP000652185
See also references of EP 2894159A4
HOBER S. ET AL., J. CHROMATOGR. B, vol. 848, 2007, pages 40 - 47
LOW D. ET AL., J. CHROMATOGR. B, vol. 848, 2007, pages 48 - 63
ROQUE A. C. A. ET AL., J. CHROMATOGR. A, vol. 1160, 2007, pages 44 - 55
HERMANSON G.T. ET AL.: "Immobilized Affinity Ligand Techniques", 1992, ACADEMIC PRESS
LJUNGQUIST C. ET AL., EUR. J. BIOCHEM., vol. 186, 1989, pages 557 - 561
Attorney, Agent or Firm:
UEKI, Kyuichi et al. (JP)
Hisakazu Ueki (JP)
Hisakazu Ueki (JP)
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