Field of the invention The invention refers to molecules able to modulate the expression of at least a gene involved in degradative pathways so to enhance the cellular degradative pathways and prevent or antagonize the accumulation of toxic compounds in a cell.

Background of the invention Lysosomes are specialized to degrade macromolecules received from the secretory, endocytic, autophagic and phagocytic pathways {1). Lysosomal storage disorders and neurodegenerative diseases such as Alzheimer's, Parkinson's, and Huntington's share as a common feature the progressive accumulation of undigested macromolecules within the cell, either proteins that tend to form pathogenic aggregates, or intermediates of the cellu lar catabolism . This ultimately results in cellu lar dysfunction and clinical manifestations with variable association of visceral (hepatosplenomegaly), skeletal (joint limitation, bone disease and deformities), hematologic (anemia, lymphocyte vacuolization and inclusions), and, most importantly, neurological involvement, with often irreversible damage and invalidating or fatal consequences. Since all of these disorders share a reduced digestive capability of the cell, it would be of great medical interest to identify molecules able to act as general enhancers of degradative pathways. Lysosomes are organelles central to degradation and recycling processes in animal cells. Whether lysosomal activity is coordinated to respond to cellular needs remains unclear. We found that most lysosomal genes exhibit coordinated transcriptional behavior and are regulated by the transcription factor TFEB. Under aberrant lysosomal storage conditions TFEB translocated from the cytoplasm to the nucleus, resulting in the activation of its target genes. TFEB overexpression in cultured cells induced lysosomal biogenesis and increased the degradation of complex molecules, such as glycosaminoglycans (GAGs) and the pathogenic protein causing Huntington disease. Thus, a genetic program controls lysosomal biogenesis and function, providing a potential therapeutic target to enhance cellular clearing in lysosomal storage disorders and neurodegenerative diseases. Prior art reports the description of a system to increase the activity of some cathepsins following the inhibition of the lysosomal system; however, these results are rather partial, controversial, and the molecular mechanism has not been analyzed into details. In the published literature there are no papers that reveal the presence of a lysosomal gene network or that identify TFEB as a possible modulator of the lysosomal activity. Description of the invention

The authors of the invention identified a gene network that comprises the genes encoding lysosomal proteins of critical importance for the degradation of toxic compounds. These proteins are involved, directly or indirectly, in a high number of human diseases. The regulatory element responsible for the modulation of these genes has been identified in their promoter sequences. Such regulatory element, which authors called CLEAR, represents itself a target for the modulation - and therefore the enhancement - of the production of the lysosomal proteins responsible for the degradation of toxic compounds. Finally, a transcription factor, called TFEB, (NCBI GenelD = 7942; nt = NM_007162.1 , protein = NP_009093.1 (aa. 1- 476 of Seq Id No. 228) and variants thereof) has been identified as a protein able to bind to the CLEAR element and to modulate the expression of target genes. Authors demonstrated that the lysosomal activity can be modulated by increasing or decreasing the amount of TFEB. In particular, the lysosomal enhancement resulting from the increase in TFEB levels is able to clear the cell from the toxic protein responsible for the neurodegenerative Huntington's disease.

The enhancement of the cellular degradative pathways by the activation of the lysosomal system may be advantageously used for the therapy of lysosomal storage disorders and of neurodegenerative diseases. Such treatment may be performed by using: 1 ) TFEB or synthetic or biotechnological derivatives thereof, as peptide fragments, chimeric peptides etc., acting directly on the CLEAR element, responsible for the modulation of the expression of lysosomal genes and other genes involved in degradative pathways, in order to enhance the cellular degradative pathways and prevent or antagonize the accumulation of toxic compounds; and/or 2) molecules, as peptides, microRNAs, microRNA inhibitors, or any other chemicals, able to act directly or indirectly on the TFEB protein or on its amount; and/or 3) vectors for gene therapy containing TFEB, microRNAs, microRNA inhibitors, or other genes able to modulate the CLEAR regulatory network, in order to enhance the cellular degradative pathways. CA 2525255 A1 describes the use of TFEB for cancer treatment and for modulating cell proliferation or differentiation.

WO 2007/070856 claims the use of TFEB for treating immune dysfunction. The document discloses the suppression of CD40L expression by blocking TFEB via interfering RNA molecules; moreover the document discloses the suppression of TFEB by TFEB-dimers. None of the above relates to the enhancement of TFEB amount/activity to target genes. Esumi Noriko et al., The Journal of Biological Chemistry 1997, 282,3,1838-1850 discloses effects of siRNA on TFEB, which correlates with the expression of VMD2. The activation of degradative pathways via the TFEB/CLEAR network is not disclosed nor suggested in the document. US2005/255450 discloses a method for screening candidate agents to identify lead compounds for the development of therapeutic agents for treatment of neurodegenerative diseases. The document discloses experiments with yeast cells, that identified several modificators of the clearance of neurotoxic peptides, suggesting that some putative human orthologs of yeast genes should act in the same way. A possible link between TFEB expression and clearance of neurotoxic peptides, in a diagnostic perspective, is suggested, with no data. As a matter of fact HMS1 , the described yeast protein, is not the yeast ortholog of TFEB.

Finally, the CLEAR regulatory element - allowing the lysosomal system modulation - is not disclosed in any prior art documents. Technologies able to enhance the lysosomal activity have not been described so far. Authors defined molecular events involved in the modulation of the lysosomal system through the regulatory element CLEAR or the TFEB protein.

In the instant invention, lysosomal storage disorders are intended as inherited diseases in which a defect in one of many proteins participating in lysosomal biogenesis or metabolism leads to the intralysosomal storage of undegraded molecules, as described in "Lysosomes", author: Paul Saftig, Landes Bioscience, 2005.

It is an object of the invention a molecule being able to enhance the cellular degradative pathways to prevent or antagonize the accumulation of toxic compounds in a cell, characterized by: a) acting either direcly or indirectly on a CLEAR element to enhance the expression of at least a gene involved in cellular degradative pathways, said CLEAR element comprising at least one repeat of a nucleotide sequence having Seq Id No. 1 10 as consensus sequence; and b) belonging to the group of: the TFEB protein, synthetic or biotechnological functional derivative thereof, peptide fragments thereof, chimeric molecules comprising the TFEB protein, synthetic or biotechnological functional derivative thereof; modulator of the TFEB protein activity and/or expression level. For the TFEB protein it is intended the NCBI GenelD = 7942; nt = NM_007162.1 , protein = NP_009093.1 (aa. 1- 476 of Seq Id No. 228), and variants thereof. In a particular aspect of the invention the CLEAR element comprises at least one repeat of a nucleotide sequence having Seq Id No. 11 1 as consensus sequence.

Preferred CLEAR elements are those comprising at least one repeat of a nucleotide sequence selected from the group from Seq Id No. 1 to Seq Id No. 109, most preferred CLEAR elements are those comprising at least one repeat of a nucleotide sequence selected from the group of: Seq Id No. 3, Seq Id No. 9, Seq Id No. 13, Seq Id No. 26, Seq

Id No. 28, Seq Id No. 30, Seq Id No. 32, Seq Id No. 34, Seq Id No. 36, Seq Id No. 47, Seq

Id No. 50, Seq Id No. 53, Seq Id No. 59, Seq Id No. 62, Seq Id No. 77, Seq Id No. 78, Seq

Id No. 84, Seq Id No. 85, Seq Id No. 88, Seq Id No. 92, Seq Id No. 94, Seq Id No. 95, Seq Id No. 98, Seq Id No. 108. Such sequences belong to genes that are responsive either by microarray and/or realtime PCR experiments.

In a particular aspect of the invention the chimeric molecule comprises the TFEB protein and a nuclear localization signal (NLS), more preferably the chimeric molecule has the sequence of Seq Id No. 228. In another particular aspect of the invention, the modulator of the TFEB protein is a microRNA or a microRNA inhibitor, preferably the modulator of the TFEB protein is the miR-128 or a miR-128 inhibitor.

In a preferred aspect, the molecule of the invention acts either direcly or indirectly on a

CLEAR element to enhance the expression of at least a gene expressing a lysosomal protein, involved in cellular degradative pathways.

In a preferred aspect, the molecule of the invention is for medical use.

In a preferred aspect, the molecule of the invention is for neurodegenerative disorders' treatments.

Neurodegenerative diseases comprise but are not limited to the following: Alzheimer's disease, Parkinson's disease, Huntington's disease, Creutzfeldt-Jakob disease,

Spinocerebellar Ataxia (SCA).

Preferably the neurodegenerative disorder belongs to the group of Alzheimer, Parkinson and Huntington diseases.

In an alternative preferred aspect, the molecule of the invention is for for lysosomal storage disorders' treatments.

Lysosomal storage disorders comprise but are not limited to the following: Activator

Deficiency/GM2 Gangliosidosis; Alpha-mannosidosis; Aspartylglucosaminuria; Cholesteryl ester storage disease; Chronic Hexosaminidase A Deficiency; Cystinosis; Danon disease;

Fabry disease; Farber disease; Fucosidosis; Galactosialidosis; Gaucher Disease (including Type I, Type II, and Type III); GM1 gangliosidosis (including Infantile, Late infantile/Juvenile, Adult/Chronic); I-Cell disease/Mucolipidosis II; Infantile Free Sialic Acid Storage Disease/ISSD; Juvenile Hexosaminidase A Deficiency; Krabbe disease (including Infantile Onset, Late Onset); Metachromatic Leukodystrophy; Pseudo-Hurler polydystrophy/Mucolipidosis INA; MPSI Hurler Syndrome; MPSI Scheie Syndrome; MPS I Hurler-Scheie Syndrome; MPS Il Hunter syndrome; Sanfilippo syndrome Type A/MPS III A; Sanfilippo syndrome Type B/MPS I I I B; Sanfilippo syndrome Type C/MPS II I C; Sanfilippo syndrome Type D/MPS III D; Morquio Type A/MPS IVA; Morquio Type B/MPS IVB; MPS IX Hyaluronidase Deficiency; MPS Vl Maroteaux-Lamy; MPS VII Sly Syndrome; Mucolipidosis I/Sialidosis; Mucolipidosis NIC; Mucolipidosis type IV; Multiple sulfatase deficiency; Niemann-Pick Disease (including Type A, Type B, and Type C); Neuronal Ceroid Lipofuscinoses, including CLN6 disease; Atypical Late Infantile, Late Onset variant; Early Juvenile Batten-Spielmeyer-Vogt/Juvenile NCL/CLN3 disease; Finnish Variant Late Infantile CLN5; Jansky-Bielschowsky disease/Late infantile CLN2/TPP1 Disease; Kufs/Adult-onset NCL/CLN4 disease; Northern Epilepsy/variant late infantile CLN8; Santavuori-Haltia/lnfantile CLN1/PPT disease; Beta-mannosidosis; Pompe disease/Glycogen storage disease type II; Pycnodysostosis; Sandhoff disease/Adult Onset/GM2 Gangliosidosis; Sandhoff disease/GM2 gangliosidosis; Infantile Sandhoff disease/GM2 gangliosidosis; Juvenile Schindler disease; SaIIa disease/Sialic Acid Storage Disease; Tay-Sachs/GM2 gangliosidosis; Wolman disease. Preferably the lysosomal storage disorder belongs to the group of Pompe disease and Multiple Sulfatase Deficiency (MSD).

It is another aspect of the invention a nucleic acid containing a sequence encoding for the molecule according as above disclosed,

It is another aspect of the invention a vector comprising under appropriate regulative sequence the above nucleic acid, preferably for gene therapy.

The invention shall be described with reference to experimental non limitating evidences.

Figure legends

Fig. 1. A regulatory gene network controlling the expression of lysosomal genes. (A) Genomic distribution of CLEAR elements (red spots) at human gene promoters. Scores are assigned based on the CLEAR position weight matrix. Blue spots indicate CLEAR elements in the promoters of lysosomal genes. Dashed box contains all the elements corresponding to the genes that were used for Gene Ontology analysis (see text). (B) Luciferase assay using constructs carrying four tandem copies of either intact (upper) or mutated (middle, mutations in red) CLEAR elements. (C) Expression analysis of lysosomal genes following TFEB overexpression and silencing. Blue bars show the fold change of the mRNA levels of lysosomal genes in TFEB- vs. pcDNA3-transfected cells. Red bars show the fold change of mRNA levels in mimic-miR-128-transfected cells vs. cells transfected with a standard control microRNA (mimic-miR-cel-67). Randomly chosen non-lysosomal genes were used as controls. Gene expression was normalized relative to GAPDH. (D) Chromatin immunoprecipitation (ChIP) analysis. The histogram shows the amount of the immunoprecipitated DNA expressed as percentage of total input DNA. Controls include promoters of housekeeping genes [ACTB, APRT, HPRT), random genes lacking CLEAR sites (TXNDC4, WIF1) and intronic sequences (int) of lysosomal genes. Lysosomal genes and controls were significantly different: Mann- Whitney-Wilcoxon test (P<10-4). All experiments in (B), (C) and (D) were performed in triplicates (data represent mean ± s.d.). (E) Confocal microscopy showing colocalization of C1 orf85-Myc (green) with the lysosomal membrane marker LAMP1 (red) in HeLa cells.

Fig. 2. TFEB overexpression induces lysosomal biogenesis. Comparison of HeLa stable transfectants of either TFEB or empty pcDNA3 vector (control). (A) Confocal microscopy after staining with an antibody against the lysosomal marker LAMP1. (B) FACS analysis after staining with lysosome-specific dye Lysotracker. The analysis was performed on four independent clones (TFEB#1-4) (see Fig. 18). Blue bars indicate the proportion of cells with fluorescence intensity greater than the indicated threshold (P4 gate). 30,000 cells per clone were analyzed. (C) Electron microscopy analysis. Thin sections exhibit more lysosome profiles (arrows) with typical ultrastructure (see details in inset corresponding to dash boxed area) in TFEB overexpressing transfectants over the control. Scale bar, 720 nm. (D) Number of lysosomes in thin sections (average ± s.e., N=20 cells). Fig. 3. The CLEAR network is activated by lysosomal storage. (A) ChIP analysis following lysosomal storage of sucrose. The histogram shows the ratio (expressed as fold change) between the amounts of FLAG-immunoprecipitated chromatin in sucrose-treated versus non-treated cells. Lysosomal genes show an average two- to three-fold increase of immunoprecipitated chromatin, whereas no significant changes are observed for control genes. (B) Expression analysis of lysosomal genes following sucrose supplementation. The diagram shows a time-course analysis of the mRNA levels of lysosomal genes and of TFEB. Gene expression was monitored by real-time qPCR and normalized relative to GAPDH. All experiments in (A) and (B) were performed at least in duplicates (data represent mean ± s.d.). (C) Immunofluorescence microscopy analysis of TFEB subcellular localization following sucrose supplementation. HeLa clones stably expressing TFEB- 3xFLAG were stained with an anti-FLAG antibody at various time points after the addition of sucrose in culture medium. (D) Immunofluorescence microscopy analysis of TFEB localization in mouse embryonic fibroblasts (MEFs) from mouse models of three different types of LSDs. MEFs from LSD or wild-type (WT) mice were transiently transfected with a TFEB-3xFLAG construct and stained with an anti-FLAG antibody. The percentages of nuclei positive for FLAG staining were estimated by examining 100 cells per cell type in two different transfection experiments (data represent mean ± s.d.). Fig. 4. TFEB enhances cellular clearance. (A) Comparison of the kinetics of GAG clearance in HeLa stable clones of either TFEB or empty pcDNA3 vector (control). The graph shows relative amounts of 3H-glucosamine incorporated into GAGs over time. 1 = 3H-glucosamine levels at time zero. Asterisk, P < 0.05. Experiments were performed in triplicates (data represent mean ± s.d .). (B and C) Clearance of polyQ expanded huntingtin (HTT) following TFEB overexpression. (B) lmmuno blot analysis of TFEB- EGFP- positive (+) and TFEB-EGFP-negative (-) HD43 cells separated by FACS 24 h after electroporation. The graph of densitometric analysis shows a strong decrease of polyQ expanded huntingtin in TFEB-EGFP-positive cells compared to controls. (C) lmmunocytochemical analysis of TFEB and HTT in HD43(Q105) cells transfected with 3xFLAG-TFEB construct showing little huntingtin staining in cells positive for 3xFLAGTFEB staining.

Fig. 5 Lysosomal genes display coordinated expression behaviour. The diagram reports a visual representation of the expression correlation of 40 lysosomal disease genes with all known lysosomal genes. Each column represents the -22,500 gene probes of the Affymetrix HG-U 133A platform ranked by their correlation of expression with the gene indicated at the top. Blue bars represent the position of lysosomal genes within the ranked lists. The analysis shows that there is an enrichment of lysosomal genes within the first 5th percentile of ranked lists of expression correlation. Fig. 6 Detailed view of the expression correlation among lysosomal genes. The columns include the first 100 gene probes of the expression correlation lists for selected lysosomal genes. Lysosomal genes are highlighted in orange. Other genes associated to the lysosomal function are highlighted in yellow. It should be noted that in a randomly ranked list the probability of finding a lysosomal gene probe is ~1 :100. Fig. 7 Logo representation of the CLEAR element. The conservation of each residue within columns is visualized as the relative height of symbols.

Fig. 8 Distribution of CLEAR elements at the promoter regions of a subset of lysosomal genes. The CLEAR elements are clustered, often in multiple copies, around the transcription start site. The legend to colour code is reported as a schematic diagram in the figure. Fig. 9 Enzymatic activities. Quantification of the activities of lysosomal enzymes β- glucosidase, cathepsin D and β-glucuronidase in HeLa cells stably overexpressing TFEB and controls. Asterisk, P < 0.05. All measures were performed in triplicates (data represent mean ± s.d.).

Fig. 10 Expression analysis of lysosomal genes following TFEB overexpression in HEK293 cells. Blue bars show the fold change of the mRNA levels of monitored genes in TFEB- vs. pcDNA3-transfected cells. Gene expression was normalized relative to GAPDH.

Fig. 11 Validation of TFEB as a target gene of miR-128 by dual luciferase assay. The 3'UTR region of TFEB was cloned into a firefly luciferase sensor construct and transfected into HeLa cells along with a Renilla Luciferase control. Luciferase activities were measured in the presence or absence of a plasmid construct containing the precursor sequence of hsa-miR-128. EZH2 and LRIG1 genes, which were not predicted targets of miR-128, were used as negative controls. All experiments were performed in triplicates (data represent mean ± s.d.). Fig. 12 Expression analysis of lysosomal genes following mimic-miR-128 transfection into HeLa cells stably expressing a TFEB transgene lacking the 3'UTR region. To verify that the downregulation of lysosomal genes following mimic-miR-128 transfection was due to TFEB silencing, mimic-miR-128 was transfected into HeLa clones stably expressing a TFEB transgene lacking the TFEB 3'UTR region, which contains the miR128 binding site. Blue bars show the fold change of monitored genes in mimic-miR-128- transfected cells vs. cells transfected with a standard control microRNA (mimic-miR-cel-67). No significant changes were observed for any of the genes tested. Gene expression was normalized relative to GAPDH.

Fig. 13 Analysis of transcriptome changes following TFEB transient transfection in HeLa cells. The graph shows a Gene Ontology analysis by 'Cellular Compartment' category of up regulated genes with false discovery rate < 0.1.

Fig. 14 Venn diagram showing the overlap between lysosomal genes and genes induced by TFEB overexpression in HeLa cells at an FDR < 0.10. The diagram shows that 20 genes, all containing CLEAR sites in their promoters, are represented in both categories. This is likely to be an underestimate as it is based on highly stringent statistical criteria and on a single cell type. A more comprehensive view of the response of lysosomal genes to TFEB induction is shown in Figure 15 (Gene Set Enrichment Analysis). Fig. 15 Gene Set Enrichment Score Analysis (GSEA) of transcriptome changes following TFEB overexpression. The graph shows the enrichment plots generated by GSEA analysis of ranked gene expression data (left: upregulated, red; right: downregulated, blue). The enrichment score is shown as a blue line, and the vertical blue bars below the plot indicate the position of lysosomal genes carrying CLEAR sites in their promoters. The analysis shows that lysosomal genes with CLEAR sites are mostly grouped in the fraction of up-regulated genes (Enrichment Score = 0.84; P<0.0001 ).

Fig. 16 FACS analysis after staining with lysosome-specific dye lysotracker of HeLa stable transfectants of TFEB (TFEB#1-4). Blue bars indicate the proportion of cells with fluorescence intensity greater than the indicated threshold (P4 gate). 30,000 cells per clone were analyzed.

Fig. 17 Microscopy analysis of MSD cells at 48 hours following the transfection of an empty vector (left) or a TFEB vector (right). The arrows indicate the storage of glycosaminoglycans in untreated MSD cells. The experiment shows that cells treated with TFEB no longer display accumulation of undigested glycosaminoglycans.

Fig. 18 Electron microscopy analysis of MSD cells at 48 hours following the transfection of an empty vector (left) or a TFEB vector (right). Untreated cells show an extensive vacuolization due to the storage of undigested glycosaminoglycans. Cells treated with TFEB show that the cellular vacuolization is largely reversed. Fig. 19 Immunofluorescence analysis of Pompe disease cells treated with a TFEB- 3xFLAG vector. Transfected cells (arrows) show a strong reversal of the extensive vacuolization found in non-transfected cells (on the right) due to the accumulation of glycogen. Fig. 20 Inhibition of miR-128 results in the transcriptional activation of the CLEAR network. Cultured HeLa cells were transfected with a specific inhibitor of miR-128 (Dharmacon) or with a standard control (inhibitor of miR-cel-167) that has no target in human cells. Real-time qPCR was performed to monitor the expression of TFEB, its lysosomal target PSAP, two housekeeping genes (HPRT and GAPDH) and two random genes (ARPP-19 and H0XA9) 48 hours after transfection. The graph shows the ratio between the expression levels of monitored genes in cells transfected with the inhibitor of miR-128 versus control. The results show an increase in the expression of both TFEB and its target PSAP, and no changes in control genes. Gene expression was normalized relative to HPRT Fig. 21 Amino acid sequence of the engineered analog of TFEB, TFEB-NLS (Seq Id No. 228). TFEB-NLS was obtained by the addition of a nuclear localization signal (NLS) at the C-terminus of the protein. The nuclear localization signal has sequence PKKKRK (underlined in the figure).

Fig. 22 TFEB-NLS localizes in the nucleus. Immunofluorescence analysis of the TFEB analog TFEB-NLS showing a complete nuclear localization of the TFEB-NLS construct. Two series of images are reported as representative of the subcellular localization of TFEB-NLS. In each series, on the left cell nuclei are stained with the DAPI dye (specific for the DNA); on the right, cells are stained for TFEB.

Material and Methods Genome Analysis

Human genomic sequences were retrieved from the Ensemble database (http://www.ensembl.org) and analyzed by using the Regulatory Sequence Analysis Tool (28). Iterative analyses led to the identification of a consensus sequence of the CLEAR element. A position weight matrix (PWM) was built by assembling all CLEAR elements found within 200 bp from the transcription start site of lysosomal genes. Human gene promoters were searched with the CLEAR PWM using the PatSer tool (28) with default parameters. Gene Ontology (GO) analyses were performed with the web tool DAVID (http://david.abcc.ncifcrf.gov) using default parameters. Only non-redundant terms with a P value ≤ 0.01 and Fold Enrichment ≥ 2 were retained. Expression correlation analysis

Expression correlation analysis was performed as previously described (29), with minor modifications. Briefly, lysosomal genes were analyzed by using the g:Sorter tool, which is part of the g:Profiler package (30). For a selected gene probe, g:Sorter can retrieve a number of most similar coexpressed profiles in a specified GEO data set. The analysis was carried out on a total of 160 heterogeneous microarray experiments, based on the HG-U 133A GeneChip array. g:Sorter was queried with the gene probes for a representative set of lysosomal genes. For each analyzed probe, the first 3% of most correlated gene probes was retrieved for each microarray data set. Subsequently, all HG- U133A gene probes were ranked based on their occurrence in the 160 different lists of most correlated genes. Genes with an equal number of occurrences were sub-ranked according to their average ranking within the various experiments. The procedure resulted in lists of gene probes ranked by their expression correlation to the investigated genes. Cell culture and transfection HeLa cells and mouse embryonic fibroblasts from mouse models of MPSII (31 ), MPSIIIA (32), and MSD (33), were grown in Dulbecco's Modified Eagle's Medium (DMEM, Euroclone), supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS, Hyclone). Where indicated, the medium was supplied with sucrose to a final concentration of 100 mM. Cells were seeded in six-well plates at 10% confluence before transfection. Transfection was performed by using PolyFect Transfection Reagent (Qiagen) or lnterferin (PolyPlus transfection) according to the manufacturer's protocols. Transfectants for full-length TFEB and TFEB-3xFLAG were selected with 1 mg/ml G418 (Sigma). For microRNA experiments, cells were transfected with 200 nM miRIDIAN Dharmacon miRNA Mimics (miR-128, or negative control cel-miR-67) and harvested after 48 h for total RNA extraction. Luciferase Assays To test the ability of the CLEAR site to promote transcription, HeLa cells were transfected with pGL3-basic luciferase reporter plasmids containing four tandem copies of either the sequence (4 x CLEAR consensus sequences as in Seq Id No. 1 11 in bold characters) Seq Id No. 112 :

CCGGGTCACGTGACCCCAGGGTCACGTGACCCTGCGGGTCACGTGACCCTGCGGG TCACGTGACCCCC or the sequence (4 x control sequences in bold characters) Seq Id No. 1 13:

CCGGGAATCGTGACCCCAGGGAATCGTGACCCTGCGGGAATCGTGACCCTGCGGG AATCGTGACCCCC. To validate TFEB as a target of mi R-128, HeLa cells were transfected with firefly luciferase reporter plasmids containing the 3'UTR regions of either TFEB or control genes (EZH2 and LRIG1) and with a psiUx plasmid (34), construct containing the precursor sequence of hsa-miR-128. Luciferase assays were performed 48 h after transfection using Dual Luciferase Reporter Assay System (Promega), normalized for transfection efficiency by cotransfected Renilla luciferase. Molecular biology

Full-length human MITF, TFE3, TFEB and TFEC were cloned into the pcDNA3.1 vector (Invitrogen). Full-length TFEB was also cloned into the p3xFLAG-CMV-10 vector. Full- length C1 orf85 was cloned into the pcDNA3.1/c-Myc vector (Invitrogen). RNA samples were obtained using either the RNeasy or the miRNeasy kit (Qiagen) according to the manufacturer's instructions. RNA was quantified using the NanoDrop 8000 (Thermo Fischer). cDNA was synthesized using QuantiTect Reverse Transcription kit (Qiagen). Chromatin lmmunoprecipitation assay (ChIP) ChIP assays were carried out using formaldehyde-fixed nuclei isolated from HeLa transfectants carrying a TFEB-3xFLAG transgene or a control HeLa cell line without any tagged transgene (mock). Each ChIP experiment required 10 ⁷ cells. ChIP was performed using the ANTI-FLAG M2 Affinity Gel (Sigma) according to the manufacturer's protocol. Quantitative real-time PCR Real-time quantitative RT-PCR on cDNAs or sonicated chromatin was carried out with the LightCycler 480 SYBR Green I mix (Roche) using the Light Cycler 480 Il detection system (Roche) with the following conditions: 95 ⁰ C, 5 min; (95 ⁰ C, 10 s; 6O ⁰ C, 10 s; 72 ⁰ C, 15 s) x 40. For expression studies the qRT-PCR results were normalized against an internal control (GAPDH). Oligonucleotide sequences are reported in Table 5. Microarray Experiments

Total RNA from TFEB-transfected HeLa cells was used to prepare cDNA for hybridization to the Affymetrix Human Gene 1.0 ST array platform. Hybridizations were performed in triplicates at the Coriell Genotyping and Microarray Center, Coriell Institute for Medical Research, Camden, NJ, USA. A false discovery rate < 0.1 was used to assess significant gene differential expressions. Gene Set Enrichment Analysis was performed as previously described (35). The cumulative distribution function was constructed by performing 1 ,000 random gene set member-ship assignments. A nominal P value < 0.01 and an FDR < 10% were used to assess the significance of the Enrichment Score (ES). Confocal imaging

Transfected HeLa cells were grown on glass coverslips for 24 h, washed with PBS containing 100 mM MgCI ₂ and 100 mM CaCI ₂ (PBS/Ca/Mg), and fixed with 4% paraformaldehyde (PFA; Sigma) for 10 min. After washing and quenching PFA with 50 mM NH ₄ CI for 15 min, cells were washed with PBS and permeabilized in blocking buffer (0.05% saponin/0.2% BSA in PBS/Ca/Mg) for 20 min. Coverslips were then incubated O/N with appropriate primary antibodies and for 1 h with Alexa-594 and Alexa-488 conjugated secondary antibodies (Molecular Probes). Coverslips were mounted on glass slides with Vectashield (Vector Laboratories). Images were taken using a confocal microscope (LSM510; Carl Zeiss, Inc.) using a Plan-Neofluar 63x immersion objective (Carl Zeiss, Inc.). Electron microscopy Control and TFEB-overexpressing HeLa cells were washed with PBS, and fixed in 1 % glutaraldehyde dissolved in 0.2 M Hepes buffer (pH 7.4) for 30 min at room temperature. The cells were then postfixed for 2 h in OsO ₄ . After dehydration in graded series of ethanol, the cells were embedded in Epon 812 (Fluka) and polymerized at 60 ⁰ C for 72 h. Thin sections were cut at the Leica EM UC6, counterstained with uranyl acetate and lead citrate. EM images were acquired from thin sections using a Philips Tecnai-12 electron microscope equipped with an ULTRA VIEW CCD digital camera (Philips, Eindhoven, The Netherlands). Quantification of lysosomes was performed using the AnalySIS software (Soft Imaging Systems GmbH, Munster, Germany). Selection of cells for quantification was based on their suitability for stereologic analysis, i.e. only cells sectioned through their central region (detected on the basis of the presence of Golgi membranes) were analyzed. Lysosomal profiles were detected on the basis of typical ultrastructural characteristics such as high electron density, presence of multiple internal luminal vesicles, concentric and myelinoid bodies. Huntingtin clearance

Huntingtin inducible striatal cells [HD43(Q105)] were cultured at 33°C in DMEM high glucose, supplemented as described previously (36). HD43(Q105) cells were electroporated with a pCIG2-TFEB vector containing an IRES2-EGFP cassette, or with an empty pCIG2 vector as a control, using a Gene Pulser Il electoporator (BioRad). Immediately after the electoporation, cells were plated in presence of 0.2 μg/ml doxycycline (Sigma) in order to induce the transgene for expanded huntingtin. Twenty-four hours post-induction, GFP-positive cells were sorted by flow cytometry using the BD FACSAria cytometer (BD Biosciences) and used for immuno blot analysis. FACS analysis

Cells were kept in 50 nM acidotropic dye LysoTracker Red DND-99 (Molecular Probes) for 40 min. Red lysosomal fluorescence of 30,000 cells per sample was determined by flow cytometry using the BD FACSAria cytometer (BD Biosciences). GAG clearance

HeLa cells were grown in RPMI medium (Gibco, Invitrogen, Grand Island) supplemented with 10% FCS in the presence of 7μCi/ml ³ H-glucosamine hydrochloride (Perkin Elmer, 37.75 Ci/mmol, Boston) for 3 days, washed extensively with PBS and chased for variable ti mes. At each time point cel ls were harvested , homogenized and subject to chromatography on Sephadex G-25 columns (GE Healthcare, Sweden) to eliminate unincorporated ³ H-glucosamine hydrochloride. The amounts of incorporated radioactivity was measured by liquid scintillation in a Beckman LS6500 counter (Beckman Instruments, Fullerton, California, USA). Immuno-blot

Cells were lysed in cold lysis buffer (20 mM Tris-HCI, pH 7.4, 150 mM NaCI, 1 % TritonX- 100) in the presence of protease inhibitors (SIGMA) for 30 min on ice. 20 mg of protein samples were separated on SDS-PAGE acrylamide gel and transferred onto nitrocellulose membrane (Amersham Pharmacia Biotech). Primary and (HRP)-conjugated antibodies were diluted in 1 % BSA TBS-T. Bands were visualized using the ECL detection reagent (Pierce) and normalized against actin. Proteins were quantified by the Bradford method. Antibodies: Huntingtin, MAb2166 (Chemicon, Temecula, CA); Actin (Sigma). Enzymatic activities Cathepsin D activity was determined with the Cathepsin D Assay Kit (Sigma) following manufacturer's instructions, β-glucosidase activity was determined by incubating cell homogenates (10 ⁷ cells, -10 μg proteins) with 5 mM 4-MU-beta-D-glucopyranoside in 0.1 M acetate buffer, pH 4.2, for 3 hrs at 37°C. β-glucuronidase activity was determined by incubating cell homogenates (2.5x10 ⁷ cells, -25 μg proteins) with 10 mM 4-MU- glucuronide in 0.2 acetate buffer, pH 4.8, for 1 hr at 37°C. Both reactions were stopped with 1 ml glycine-carbonate buffer, pH 10.7. Fluorescence was read at 365 nm (excitation) and 450 nm (emission) on a Turner Modulus fluorometer. Data analysis

Most data are presented as the mean ± s.d. Statistical comparisons were made using analysis of variance (ANOVA). A P value < 0.05 was considered statistically significant.

Results

As stated above, lysosomes are specialized to degrade macromolecules received from the secretory, endocytic, autophagic and phagocytic pathways {1). As degradation requirements of the cell may vary depending on tissue type, age, and environmental conditions, authors postulated the presence of a cellular program coordinating lysosomal activity. By using the g:profiler (2) tool authors observed that genes encoding lysosomal proteins, hereafter referred to as lysosomal genes, tend to have coordinated expression (Figs. 5 and 6). Pattern discovery analysis of the promoter regions of the 96 known lysosomal genes (3) resulted in the identification of a palindromic 10-bp GTCACGTGAC motif highly enriched in this promoter set (68 genes out of 96; P < 0.0001 ) (Fig. 7). This motif is preferentially located within 200 bp from the transcription start site (TSS), either as a single sequence or as tandem multiple copies (Fig. 8 and Table 1 ). The distribution of this motif was determined around all human gene TSSs (Fig. 1A) and gene ontology analysis of the genes with at least two motifs within 200 bp from the TSS -suggesting they are likely in a promoter— showed a significant enrichment for functional categories related to lysosomal biogenesis and function (Table 2). Thus, authors named this motif Coordinated Lysosomal Expression And Regulation (CLEAR) element. A luciferase assay showed that the CLEAR element mediates transcriptional activation (Fig. 1 B). The CLEAR consensus sequence shown as Seq Id No. 1 10 overlaps that of the E-box (CANNTG), a known target site for bHLH transcription factors (4). In particular, members of the MiT/TFE subfamily of bHLH factors were found to bind sequences similar to the CLEAR consensus (5). The MiT/TFE subfamily is composed of four members in humans: MITF, TFE3, TFEB, and TFEC (6). To determine whether any of these proteins are able to modulate the expression of lysosomal genes, authors transfected HeLa cells with plasmids carrying MITF, TFE3, TFEB, or TFEC cDNAs. Authors observed an increase in the mRNA levels of lysosomal genes (22 out of 23 genes tested) only following TFEB overexpression (Fig. 1 C). Accordingly, authors detected a significant increase in the activities of lysosomal enzymes β-glucosidase, Cathepsin D and β-glucuronidase (Fig. 9). Induction of lysosomal genes following TFEB overexpression was also observed in HEK293 cells (Fig. 10). Authors predicted that TFEB could be a target of the micro-RNA miR-128 (7), which was confirmed by luciferase experiments (Fig. 1 1 ). MicroRNA- mediated TFEB silencing was associated with the downregulation of 18 out of the 23 lysosomal genes tested (Figs. 1 C and 12). Thus, TFEB regulates the expression of lysosomal genes.

The inhibition of miR-128, performed with a specific miRNA inhibitor (Dharmacon), resulted in the increase of the expression of TFEB and of its target lysosomal gene PSAP (Fig. 20), demonstrating that the modulation of the expression of miR-128 can directly influence the activation of the CLEAR network.

To test whether lysosomal genes are direct targets of TFEB authors performed chromatin immunoprecipitation (ChIP) analysis on HeLa cells stably expressing a TFEB 3xFLAG construct using an anti-FLAG antibody. The results demonstrated that TFEB binds to CLEAR sites (Fig. 1 D). To identify genes responsive to TFEB on a genomic scale authors performed microarray analysis of the HeLa transcriptome following TFEB overexpression. Authors observed that 291 genes were up-regulated, and 7 down-regulated, at a false discovery rate < 0.1 (Table 3). Up-regulated genes were greatly enriched with lysosomal genes and genes related to lysosomal biogenesis and function (Figs. 13 and 14, Table 4). Accordingly, Gene Set Enrichment Analysis (GSEA) showed a significant enrichment (Enrichment Score = 0.84; P < 0.0001 ) of lysosomal genes that contain CLEAR elements in their promoters among induced genes (Fig. 15). Interestingly, non-lysosomal genes involved in degradation pathways appear to be modulated by TFEB. These include: RRAGC and UVRAG, which are key factors regulating autophagy (8, 9); CSTB, which plays a role in protecting against the proteases leaking from lysosomes {10); M6PR and IGF2R, which mediate the import of proteins into the lysosome (11). To illustrate the feasibility of using the CLEAR network as a tool to identify genes involved in lysosomal function and to provide candidate genes for orphan lysosomal diseases (3), authors determined the subcellular distribution of two randomly chosen proteins of unknown function, C1orf85 and C12orf49. The uncharacterized TFEB target, C1orf85, was found localized to lysosomes (Fig. 1 E).

An expansion of the lysosomal compartment was detected in HeLa transfectants stably overexpressing TFEB (Fig. 2, A and B and Fig. 16). Accordingly, ultrastructural analysis revealed a significant increase in the number of lysosomes per cell (Fig. 2, C and D), indicating the involvement of TFEB in lysosomal biogenesis. Authors used a sucrose-induced vacuolization model {12, 13) to test whether the TFEB- CLEAR network responds to lysosomal storage of undegraded molecules. An increase of the binding events of TFEB to lysosomal promoters (Fig. 3A) and of the mRNA levels of lysosomal genes, and to a lesser extent of TFEB, was detected upon sucrose supplementation to the culture medium (Fig. 3B). The addition of sucrose also determined the progressive translocation of TFEB from a diffuse localization in the cytoplasm, where it predominantly resides in untreated cells, to the nucleus (Fig. 3C), suggesting that nuclear translocation is an important mechanism for TFEB activation. Over 40 lysosomal storage disorders (LSDs) are characterized by the progressive accumulation of undigested macromolecules within the cell, resulting in cellular dysfunction that leads to diverse clinical manifestations {1, 14, 15). Authors investigated TFEB subcellular localization in embryonic fibroblasts obtained from mouse models of three different LSDs, Mucopolysaccharidoses types Il and INA (MPSII and MPSIIIA) and Multiple Sulfatase Deficiency (MSD) (16-18). A predominant nuclear localization of TFEB was detected in cells from all three LSD mouse models (Fig. 3D), suggesting that the TFEB signaling pathway is activated following the intra-lysosomal storage of undegraded molecules. Such activation could be part of the cellular physiological response to lysosomal stress and could serve degradation needs by enhancing the lysosomal system. In order to obtain a TFEB molecule able to completely and directly localize into the nucleus, authors designed a TFEB analog (chimeric molecule) by adding a nuclear localization signal (NLS) at the C-terminus of the TFEB protein (Seq Id No. 228, Fig. 21 ). lmmunofuorescence analysis of HeLa cells transfected with the TFEB-NLS construct demonstrated that it indeed localize into the nucleus (Fig. 22), with no needs for storage conditions. Lysosomal storage disorders are caused by the intracellular accumulation of undigested material due to mutations in genes participating to lysosomal function. In Multiple Sulfatase Deficiency (MSD), a severe human disorder, a defect in sulfatases impairs the ability of the cell to degrade sulfated compounds, with the subsequent accumulation of glycosaminoglycans that induce extensive cellular vacuolization and finally prove to be toxic for the cells. Authors used cells derived from a mouse model of MSD to test the clearance capability of TFEB in this disease. They transfected MSD cells with a TFEB vector or an empty vector and monitored the accumulation of glycosaminoglycans 48 hours post-transfection. They found that TFEB was able to promote the clearance of stored glycosaminoglycans (Fig. 17) and to reverse the subsequent cellular vacuolization, as demonstrated by electron microscopy analysis (Fig. 18). Authors tested the clearance capability of TFEB on an additional model of lysosomal storage disorder, the Pompe disease, in which a defect in the acid alpha-glucosidase gene leads to the intralysosomal accumulation of glycogen and subsequent extensive vacuolization of the cell. Authors transfected human fibroblasts derived from a Pompe patient with a TFEB-3xFLAG vector and monitored the shape and the number of lysosomes in the cells. Cells transfected with TFEB-3xFLAG were fou nd to d iminish the amou nt of und igested glycogen , as demonstrated by the decreased number of lysosomal vesicles compared to non- transfected cells (Fig. 19). Together, these data indicate that the enhancement of the lysosomal activity by acting on the CLEAR network can provide in principle a polyvalent therapy against different lysosomal storage disorders. To test the ability of TFEB to enhance lysosome-dependent degradation pathways authors analyzed the degradation of glycosaminoglycans (GAGs) in a pulse-chase experiment. TFEB stable transfectants displayed a faster rate of GAG clearance compared to controls (Fig. 4A). Authors also investigated the ability of TFEB to induce the degradation of the polyglutamine (polyQ) expanded huntingtin protein responsible for Huntington disease using the rat striatal cell model H D43 that carries an inducible transgene for mutant huntingtin (79). lmmunoblot analyses showed a strong decrease of mutant huntingtin in TFEB-overexpressing cells compared to controls (Fig. 4B). In a parallel experiment, induced HD43 cells were electroporated with a 3xFLAG-TFEB construct. Immunofluorescence analyses showed that the cells that are positive for 3xFLAG-TFEB show little, if any, huntingtin accumulation (Fig. 4C).

Authors have discovered a cellular program that regulates lysosomal biogenesis and participates in macromolecule clearance. Lysosomal enhancement as a cellular response to pathogenic accumulation has been observed in neurodegenerative diseases (20-22). Interestingly, cathepsin D (23, 24), one of the key enzymes involved in the degradation of neu rotoxic protei ns, belongs to the CLEAR network and is induced by TFE B overexpression. Of particular interest is also the observation that miR-128, which authors used for TFEB downregulation, is significantly up-regulated in the brain of patients with Alzheimer's disease (25) and in both prion- and chemical-induced neurodegeneration (26, 27). An appealing perspective would be the use of the CLEAR network as a therapeutic target to enhance cellular response to intracellular pathogenic accumulation in neurodegenerative diseases.

Table 1. Distribution of CLEAR elements in the promoters of human lysosomal genes.

Gene Seq Id symbol Gene name CLEAR element Position* No. Membrane transporters ABCA2 ATP-binding cassette, sub-family A (ABC1 ), GTCGCGTGAC -187 1 member 2 ABCB9 ATP-binding cassette, sub-family B (MDR/TAP), CTCACCTGGT 94 2 member 9 CLCN7 chloride channel 7 ATCACGTGGC -103 3

GTCACGTGGC -83 4

CLN3 ceroid-lipofuscinosis, neuronal 3, juvenile AGCACGTGAT -24 5

GTCACGTGAT 6 6

CLN5 ceroid-lipofuscinosis, neuronal 5 CTCAAGTGTG 50 7

TTCAGGTGCC 74 8

CTNS cystinosis, nephropathic GTCAGGTGGC -32 9

GTCAGGTGAC -18 10

LAPTM4A lysosomal-associated protein transmembrane 4 GTCACGTTAT -372 11 alpha

GTGACGCTTC -356 12

LMBRD1 LMBR1 domain containing 1 MCOLN1 mucolipin 1 GTCACGTGAG -47 13

GTCACGTGAC -20 14

ATCAGCTGAT 0 15

MFSD8 major facilitator superfamily domain containing 8 GTCAGGTGCG -15 16

NPC1 Niemann-Pick disease, type C1 TTCAGGTGAC -383 17

SCARB2 scavenger receptor class B, member 2 CTCAGGCGCC -134 18

GGCACATGAC -57 19

SLC17A5 s o l u te ca rri e r fa m i ly 1 7 ( a n i o n /s u g a r GCCAGGTGGC 47 20 transporter), member 5

CTCACGTAGG 68 21

SLC36A1 solute carrier family 36 (proton/amino acid AGCACGTGAC -44 22 symporter), member 1

ATCACGTGAT -9 23

Hydrolases

ACP2 acid phosphatase 2

ACP5 acid phosphatase 5, tartrate resistant CTCACCTGGG 24

AGA aspartylglucosaminidase

ARSA arylsulfatase A GCCAAGTGAC 80 25

ARSB arylsulfatase B GTCAGCTGAG 288 26

ARSG arylsulfatase G GCCACGTGTG 183 27

ASAH 1 N-acylsphingosine amidohydrolase 1 GTCACGCGGC -41 28

CPVL carboxypeptidase, vitellogenic-like GTCATGTGAG -123 29

CTBS di-N-acetyl-chitobiase

CTSA cathepsin A GTCACGTGGC -50 30

TTCACGTGAC -33 31

CTSB cathepsin B GTCACGTGGG -7 32

CTSC cathepsin C TTCACCTGAC -343 33 CTSD cathepsin D CCCACGTGAC 16 34

GTCAGCTGAT 48 35

CTSF cathepsin F CCCACGTGCC -83 36

CTSH cathepsin H CCCAGTTGAC 30 37

CTSK cathepsin K GTCACATGTG -650 38

TTCAAGTGCT -615 39

CTSL1 cathepsin L1 GTCAGGCGAA 43 40

CTSS cathepsin S CTCAAGTGAT -66 41

CTSZ cathepsin Z TTCAGGTGCC -166 42

DNASE2 deoxyribonuclease II, lysosomal GCCAGGTGCC 63 43

ENTPD4 ectonucleoside triphosphate - - diphosphohydrolase 4

FUCA1 alpha-L fucosidase - -

GAA alpha-glucosidase GTCACGTGAC 20 44

GTCACGTGAC 65 45

GALC galactosylceramidase GTCATGTGAC 1 46

GALNS galactosamine (N-acetyl)-6-sulfate sulfatase GTCACGTGAC -147 47

GTCACGCGGC -128 48

GTCACGTGGC -5 49

GBA beta -glucosidase GTCATGTGAC -64 50

ATCACATGAC -44 51

GGH gamma-glutamyl hydrolase CTCACGCGAG -31 52

GLA alpha-galactosidase CTCACGTAAG -223 53

ATCACGTGAG -207 54

GTCATGTGAG -190 55

GTCACGTGAG -174 56

GLB1 beta-galactosidase GTCACGCGGC -139 57

GTCAAGTGAC -3 58

GNS glucosamine (N-acetyl)-6-sulfatase GTCACGTGAC -42 59

CTCACGTGAT -2 60

GUSB beta-glucuronidase GTCACGCGAC -49 61

HEXA beta-hexosaminidase subunit alpha GTCACGTGAT -3 62

CTCACCTGAC 33 63

CTCACGTGGC 49 64

HEXB beta-hexosaminidase subunit beta GTCATCTGAC 3 65

HGSNAT heparan-alpha-glucosaminide N- - - acetyltransferase

HPSE Heparanase GCCAGGTGAG 84 66

HYAL1 hyaluronoglucosaminidase 1 - -

HYAL2 hyaluronoglucosaminidase 2 GTCACCTGGC -194 67

IDS lduronate-2-sulfatase - -

IDUA alpha-L-iduronidase GTCACATGGG 1 68

LGMN legumain - -

LIPA acid lipase ATCAGATGCC 34 69 LYPLA3 lysophospholipase 3 GTCACCTGAG -431 70

MAN2B1 alpha-mannosidase, class 2B, member 1 CTCCCGTGAG -87 71

MAN2B2 alpha-mannosidase, class 2B, member 2

MANBA beta-mannosidase CTCAGCTGAC -47 72

NAAA N-acylethanolamine acid amidase

NAGA alpha-N-acetylgalactosaminidase CCTTCGTGAG -23 73

CTCACTGGAA -5 74

ATCAGGTTAC 18 75

GTCAGAAGCG 37 76

NAGLU alpha-N-acetylglucosaminidase GTCACGAGAC 178 77

NEU1 sialidase 1 GTCACGCGCT -116 78

GTCAGCTGAC 69 79

NEU4 sialidase 4 GTCATTTGAG -336 80

P76 mannose-6-phosphate protein p76 GTCACGTGAC -12 81

PPT1 palmitoyl-protein thioesterase 1 GTCATGTGAC 39 82

PPT2 pa I mitoyl- protein thioesterase 2

RNASET2 ribonuclease 6 GGCAGGTGAG -41 83

SCPEP1 serine carboxy peptidase 1 GTCACGTGAT -26 84

SGSH N-sulfoglucosamine sulfohydrolase CGCACGTGAC -85 85

SIAE sialic acid acetylesterase

SMPD1 sphingomyelin phosphodiesterase ATCAGCTGTC -14 86

GTCAGCCGAC 51 87

TMEM55B transmembrane protein 55B AACACGTGAC -288 88

GTCACGTGCA -193 89

GTCATGTGAC -154 90

ATCACGTGCT -36 91

TPP1 tripeptidyl peptidase I CTCATGTGAT -15 92

GTCACATGAC -3 93

Signaling

CREG1 cellular repressor of E1 A-stimulated genes 1

LITAF lipopolysaccharide-induced TNF factor

TMEM9 transmembrane protein 9

Other functions

CD63 CD63 molecule GTCACATGAG 14 94

CD68 CD68 molecule TCAACTGCCC -82 95

CCCATGTGAC -55 96

GM2A GM2 ganglioside activator

IFI30 interferon, gamma-inducible protein 30 CTCACGTGCC -174 97

LAMP1 lysosomal-associated membrane protein 1 GTCACGTGGG -196 98

GTCACGTGCC -180 99

GTCACGTGCC -163 100

GTCACGTGTC -146 101

ATCACGTGAC -32 102

CTCACGTGAC -5 103 LAMP2 lysosomal-associated membrane protein 2

LAMP3 lysosomal-associated membrane protein 3

MPO myeloperoxidase ATCAGGTGAG 7 104

NCSTN nicastrin

NPC2 Niemann-Pick disease, type C2 CTCAGCTGTG -19 105

GTCGCCTGAC 5 106

GTCTTGTGAC 49 107

OSTM1 osteopetrosis associated transmem brane protein 1

PCYOX1 prenylcysteine oxidase 1 PSAP prosaposin ATCAGCTGAC 108

TMEM74 transmembrane protein 74 Unknown function

C2orf18 chromosome 2 open reading frame 18 GTCACGTGAC -33 109

C7orf28A chromosome 7 open reading frame 28A EPDR1 ependymin related protein 1

LAPTM5 lysosomal-associated multispanning membrane protein 5 TMEM92 transmembrane protein 92

*Position refers to the transcription start site

Table 2. Gene Ontology (GO) analysis of CLEAR genes.

GO Term Gene Fold P value Count enr.

Cellular Compartment

GO:0005764~lysosome 23 7.2 1.03E-12

GO:0016471 -vacuolar proton-transporting V-type ATPase 3 37.3 2.48E-03 complex

GO:0005768~endosome 10 3.2 4.34E-03

Biological Process

GO:0007040~lysosome organization and biogenesis 7 24.3 2.56E-07

GO:0016192~vesicle-mediated transport 20 2.6 2.73E-04

GO:0032940~secretion by cell 13 3 1.43E-03

GO:0006643~membrane lipid metabolic process 11 3.4 1.56E-03

GO:0046034~ATP metabolic process 6 6.7 1.94E-03

GO:0006644~phospholipid metabolic process 9 3.7 3.12E-03

GO:0045045~secretory pathway 11 3 3.49E-03

Molecular Function

GO:0016787~hydrolase activity 58 1.6 2.39E-04

GO:0016298~lipase activity 8 5.3 7.98E-04

GO:0016798~hydrolase activity, acting on glycosyl bonds 9 4.2 1.37E-03

GO:0016805~dipeptidase activity 3 20.2 9.07E-03 Table 3. Genes differentially expressed following TFEB transient overexpression.

Gene Symbol Protein Process Fold change

ATP6V0D2 ATPase, H+ transporting, lysosomal 38kDa, VO Lysosomal 2908 subunit d2 acidification RASGRP3 RAS guanyl releasing protein 3 (calcium and DAG- Signal transduction 92.8 regulated)

ZNF57 zinc finger protein 57 unknown 60.7

TRIM63 tripartite motif-containing 63 Protein degradation 40.6

SLC16A6 solute carrier family 16, member 6 (monocarboxylic Drug disposition 38.5 acid transporter 7)

PER3 period homolog 3 (Drosophila) Circadian rhythms 37.7

TM4SF19 transmembrane 4 L six family member 19 unknown 23.6

CPA2 carboxypeptidase A2 (pancreatic) Protein degradation 19.4

C1orf54 chromosome 1 open reading frame 54 unknown 17.2

SULT1 C2 sulfotransferase family, cytosolic, 1 C, member 2 Sulfate conjugation 13.9

CTNS cystinosis, nephropathic Lysosomal carrier 13.6

NR1 D1 nuclear receptor subfamily 1 , group D, member 1 Circadian rhythms 12.5

UCA1 urothelial cancer associated 1 unknown 12.3

UPP1 uridine phosphorylase 1 Catabolism of 11.1 nucleotides

SLC19A2 solute carrier family 19 (thiamine transporter), Thiamin transport 10.3 member 2

GPR56 G protein-coupled receptor 56 Signal transduction 9.8

SLAMF7 SLAM family member 7 Immune response 9.6

PRKAG2 protein kinase, AMP-activated, gamma 2 non- Energy metabolism 8.6 catalytic subunit

STS steroid sulfatase (microsomal), isozyme S Microsomal hydrolase 8.4

CCRL2 similar to chemokine (C-C motif) receptor-like 2 Immune response 8.3

MAP3K13 mitogen-activated protein kinase kinase kinase 13 Signal transduction 7.8

GIPR gastric inhibitory polypeptide receptor Insulin metabolism 7.6

SEMA3D sema domain, immunoglobulin domain (Ig), short Signal transduction 7.4 basic domain, secreted, (semaphorin) 3D ANKRD1 ankyrin repeat domain 1 (cardiac muscle) Signal transduction 7.2

BHLHB3 basic helix-loop-helix domain containing, class B, 3 Circadian rhythms 6.8

VASN vasorin Signal transduction 6.5

PTP4A3 protein tyrosine phosphatase type IVA, member 3 Cell growth 6.4

FNIP2 folliculin interacting protein 2 unknown 6.3

PLK3 polo-like kinase 3 (Drosophila) Protein 6.2 phosphorylation

CPA4 carboxypeptidase A4 Protein degradation 6.1

ST3GAL1 ST3 beta-galactoside alpha-2,3-sialyltransferase 1 Protein glycosylation 6.1 CSF1 R colony stimulating factor 1 receptor, formerly Immune response 5.8

McDonough feline sarcoma viral (v-fms) oncogene homolog SUV39H1 suppressor of variegation 3-9 homolog 1 Chromatin 5.7

(Drosophila) modification

ZDHHC3 zinc finger, DHHC-type containing 3 unknown 5.5

IL6R interleukin 6 receptor Immune response 5.5

FAM27E3 family with sequence similarity 27, member E3 unknown 5.5

C1 R complement component 1 , r subcomponent Immune response 5.5

FAM102A family with sequence similarity 102, member A unknown 5.4

SECTM1 secreted and transmembrane 1 Immune response 5.4

FAM124A family with sequence similarity 124A unknown 5.3

RGS16 regulator of G-protein signaling 16 Signal transduction 5.3

RASD2 RASD family, member 2 Signal transduction 5.3

PLCXD1 phosphatidylinositol-specific phospholipase C, X unknown 5.2 domain containing 1

AHNAK2 AHNAK nudeoprotein 2 unknown 5.1

ASAH1 N-acylsphingosine amidohydrolase (acid Lysosomal hydrolase 5.1 ceramidase) 1

SLC26A11 solute carrier family 26, member 11 Sulfate transport 5.1

TMEIV180 transmembrane protein 80 unknown 5.1

HEXA hexosaminidase A (alpha polypeptide) Lysosomal hydrolase 5.1

SLC26A9 solute carrier family 26, member 9 Sulfate transport 5.0

TGM5 transglutaminase 5 Epidermis 5.0 development

MCOLN1 mucolipin 1 Lysosomal carrier 5.0

FLJ41484 hypothetical LOC650669 unknown 5.0

ALOXE3 arachidonate lipoxygenase 3 Inflammatory 4.9 response

CHKA choline kinase alpha Lipid metabolism 4.9

C17orf80 chromosome 17 open reading frame 80 unknown 4.7

LIF leukemia inhibitory factor (cholinergic differentiation Immune response 4.6 factor) ADFP adipose differentiation-related protein Adipocyte 4.6 differentiation

SLC20A1 solute carrier family 20 (phosphate transporter), Sulfate transport 4.6 member 1

DKFZp451A211 DKFZp451A211 protein unknown 4.6 ATP6V0D1 ATPase, H+ transporting, lysosomal 38kDa, VO Lysosomal 4.5 subunit d1 acidification

DEXI dexamethasone-induced transcript unknown 4.4

FAM21 B family with sequence similarity 21 , member B unknown 4.4

PLEKHM1 pleckstrin homology domain containing, family M Lysosomal 4.4

(with RUN domain) member 1 metabolism CEP72 centrosomal protein 72kDa Centrosome 4.3 component

DVL2 dishevelled, dsh homolog 2 (Drosophila) Signal transduction 4.3

SNAI2 snail homolog 2 (Drosophila) Development 4.3

LSS lanosterol synthase (2,3-oxidosqualene-lanosterol Cholesterol 4.2 cyclase) metabolism

HSPC159 galectin-related protein unknown 4.2

RAET1 E retinoic acid early transcript 1 E Immune response 4.2

TCTEX1 D2 Tctexi domain containing 2 unknown 4.2

SERTAD2 SERTA domain containing 2 Cell proliferation 4.2

LOC201164 similar to CG12314 gene product unknown 4.1

TMEFF1 transmembrane protein with EGF-like and two Signal transduction 4.1 follistatin-like domains 1

VPS18 vacuolar protein sorting 18 homolog (S. cerevisiae) Lysosomal trafficking 4.1

SYNJ2 synaptojanin 2 Metabolism 4.1

LOC100132929 similar to hCG24378 unknown 4.1

HLA-B major histocompatibility complex, class I, B Proteasome 4.1 degradation

CRYAB crystallin, alpha B Apoptosis 4.1

CABLES1 Cdk5 and AbI enzyme substrate 1 Cell proliferation and 4.0 differentiation

GRN granulin Inflammatory 4.0 response

UVRAG UV radiation resistance associated gene Autophagy 4.0

CAMKK1 calcium/calmodulin-dependent protein kinase kinase Immune response 4.0

1 , alpha

SPINK1 serine peptidase inhibitor, Kazal type 1 Protease inhibitor 4.0

CLEC17A C-type lectin and transmembrane domain- unknown 4.0 containing protein FLJ45910

PPARGC1A peroxisome proliferator-activated receptor gamma, Energy metabolism 3.9 coactivator 1 alpha

TPP1 tripeptidyl peptidase I Lysosomal hydrolase 3.9

SFXN3 sideroflexin 3 Mitochondrial carrier 3.9

HES1 hairy and enhancer of split 1 , (Drosophila) Development 3.9

EIF2C4 eukaryotic translation initiation factor 2C, 4 Gene silencing 3.9

VPS11 vacuolar protein sorting 11 homolog (S. cerevisiae) Lysosomal trafficking 3.9

CTSF cathepsin F Lysosomal hydrolase 3.9

KCNAB2 potassium voltage-gated channel, shaker-related unknown 3.8 subfamily, beta member 2

SETDB2 SET domain, bifurcated 2 Chromatin 3.8 modification

PSG4 pregnancy specific beta-1 -glycoprotein 4 Defense response 3.8

C12orf49 chromosome 12 open reading frame 49 unknown 3.8

BLVRB biliverdin reductase B (flavin reductase (NADPH)) Metabolism 3.8 APBB3 amyloid beta (A4) precursor protein-binding, family APP metabolism 3.8

B, member 3

UCK1 uridine-cytidine kinase 1 Metabolism 3.7

HSPB8 heat shock 22kDa protein 8 Cell proliferation 3.7

LRRC8B leucine rich repeat containing 8 family, member B unknown 3.7

NHEDC2 Na+/H+ exchanger domain containing 2 Mitochondrial carrier 3.7

TIAF1 TGFB1-induced anti-apoptotic factor 1 Apoptosis 3.7

FAM21A family with sequence similarity 21 , member A unknown 3.7

STOM stomatin unknown 3.7

HEY1 hairy/enhancer-of-split related with YRPW motif 1 Development 3.6

BHLHB2 basic helix-loop-helix domain containing, class B, 2 Development 3.6

NUP50 nucleoporin 5OkDa Nuclear pore 3.6 component

WDR81 WD repeat domain 81 unknown 3.6 ACBD3 acyl-Coenzyme A binding domain containing 3 Golgi transport 3.6 FBXO32 F- box protein 32 Ubiquitylation 3.6 GEM GTP binding protein overexpressed in skeletal Signal transduction 3.6 muscle

UGDH UDP-glucose dehydrogenase Biosiynthesis of 3.6

GAGs HOXB9 homeobox B9 Cell proliferation and 3.6 differentiation

LOC100128975 similar to Zinc finger protein 626 unknown 3.6

LYPD5 LY6/PLAUR domain containing 5 Signal transduction 3.6

CLC Charcot-Leyden crystal protein Lipid metabolism 3.6

CD22 CD22 molecule Immune response 3.5

NIT1 nitrilase 1 Metabolism 3.5

SRRD SRR1 domain containing unknown 3.5

VEGFA vascular endothelial growth factor A Development 3.5

MMP12 matrix metallopeptidase 12 (macrophage elastase) Protein degradation 3.5

LAMA1 laminin, alpha 1 Cell proliferation and 3.5 differentiation

HMOX1 heme oxygenase (decycling) 1 Metabolism 3.5 SLC25A16 solute carrier family 25 (mitochondrial carrier; Mitochondrial carrier 3.5

Graves disease autoantigen), member 16

KIAA1632 KIAA1632 unknown 3.5

HK2 hexokinase 2 Energy metabolism 3.5

KIFC3 kinesin family member C3 Golgi organization 3.5 and biogenesis

CD68 CD68 molecule Lysosomal 3.5 metabolism

CHUK conserved helix-loop-helix ubiquitous kinase Immune response 3.5 RAB17 Ras-related protein Rab-17 Signal transduction 3.5 CXCL16 chemokine (C-X-C motif) ligand 16 Immune response 3.5 KIAA1737 KIAA1737 unknown 3.4

CRY1 cryptochrome 1 (photolyase-like) Circadian rhythms 3.4

NDRG1 N-myc downstream regulated gene 1 Cell proliferation and 3.4 differentiation

NEDD4L neural precursor cell expressed, developmentally Ubiquitylation 3.4 down-regulated 4-like

KCNN4 potassium intermediate/small conductance calcium- Defense response 3.4 activated channel, subfamily N, member 4 NAGK N-acetylglucosamine kinase Metabolism 3.4

FAM54A family with sequence similarity 54, member A unknown 3.4

PSEN2 presenilin 2 (Alzheimer disease 4) APP metabolism 3.4

PPIF peptidylprolyl isomerase F (cydophilin F) Mitochondrial 3.4 metabolism

LOC654433 hypothetical LOC654433 unknown 3.4

DCPS decapping enzyme, scavenger mRNA metabolism 3.4

PDXDC2 pyridoxal-dependent decarboxylase domain Metabolism 3.4 containing 2 PLCD1 phospholipase C, delta 1 Phospholipid 3.4 metabolic process

STK19 serine/threonine kinase 19 unknown 3.4

LCN8 lipocalin 8 Metabolism 3.4

DUSP10 dual specificity phosphatase 10 Signal transduction 3.3

SBNO2 strawberry notch homolog 2 (Drosophila) Immune response 3.3

LY6K lymphocyte antigen 6 complex, locus K unknown 3.3

GSTO1 glutathione S-transferase omega 1 Metabolism 3.3

SLC29A1 solute carrier family 29 (nucleoside transporters), Metabolism 3.3 member 1

CD300C CD300c molecule Immune response 3.3

AVPH arginine vasopressin-induced 1 unknown 3.3

DAB2 disabled homolog 2, mitogen-responsive Lysosomal trafficking 3.3 phosphoprotein (Drosophila) SLCO4A1 solute carrier organic anion transporter family, unknown 3.3 member 4A1

GSR glutathione reductase Metabolism 3.3

UST uronyl-2-sulfotransferase Metabolism 3.3

PTTG1 IP pituitary tumor-transforming 1 interacting protein Signal transduction 3.3

ICAM1 intercellular adhesion molecule 1 (CD54), human Immune response 3.3 rhinovirus receptor NUFIP1 nuclear fragile X mental retardation protein Transcription 3.3 interacting protein 1

RAB3IL1 RAB3A interacting protein (rabin3)-like 1 Exocytosis 3.3

TEAD3 TEA domain family member 3 Pregnancy 3.2

GDF15 growth differentiation factor 15 Signal transduction 3.2

PIM1 pim-1 oncogene Cell proliferation 3.2 TAF4B TAF4b RNA polymerase II, TATA box binding Transcription 3.2 protein (TBP)-associated factor, 105kDa

MFSD1 major facilitator superfamily domain containing 1 unknown 3.2

CTSB cathepsin B Lysosomal hydrolase 3.2

EPS15L1 epidermal growth factor receptor pathway substrate Endocytosis 3.2

15-like 1

SPTBN1 spectrin, beta, non-erythrocytic 1 Cytoskeleton 3.2 component

CSTB cystatin B (stefin B) Protease inhibitor 3.2

HKDC1 hexokinase domain containing 1 Energy metabolism 3.2

LPAR5 lysophosphatidic acid receptor 5 Signal transduction 3.2

CTSD cathepsin D Lysosomal hydrolase 3.2

LINS1 lines homolog 1 (Drosophila) unknown 3.2

IGF2R insulin-like growth factor 2 receptor Lysosomal trafficking 3.2

RCSD1 RCSD domain containing 1 unknown 3.2

CSPG4 chondroitin sulfate proteoglycan 4 Signal transduction 3.2

VAC14 Vac14 homolog (S. cerevisiae) Signal transduction 3.2

CHRM4 cholinergic receptor, muscarinic 4 Signal transduction 3.2

IL16 interleukin 16 (lymphocyte chemoattractant factor) Immune response 3.2

SLC25A40 solute carrier family 25, member 40 Mitochondrial carrier 3.2

MTMR10 myotubularin related protein 10 Signal transduction 3.2

RLTPR RGD motif, leucine rich repeats, tropomodulin unknown 3.2 domain and proline-rich containing SH3RF2 SH3 domain containing ring finger 2 Ubiquitylation 3.1

PFKFB3 6-phosphofructo-2-kinase/fructose-2,6- Energy metabolism 3.1 biphosphatase 3

TMEM16B transmembrane protein 16B unknown 3.1

DENND2D DENN/MADD domain containing 2D unknown 3.1

ADM adrenomedullin Signal transduction 3.1

SLC25A25 solute carrier family 25 (mitochondrial carrier; Mitochondrial carrier 3.1 phosphate carrier), member 25 SLC2A1 solute carrier family 2 (facilitated glucose Glucose transporter 3.1 transporter), member 1 ATP6V0B ATPase, H+ transporting, lysosomal 21 kDa, VO Lysosomal 3.1 subunit b acidification

TOM1 target of myb1 (chicken) Endocytic trafficking 3.1

DDI2 DDH , DNA-damage inducible 1 , homolog 2 (S. Protein degradation 3.1 cerevisiae) SLC25A22 solute carrier family 25 (mitochondrial carrier: Mitochondrial carrier 3.1 glutamate), member 22 NAPA N-ethylmaleimide-sensitive factor attachment ER-Golgi transport 3.1 protein, alpha ESCO1 Establishment of cohesion 1 homolog 1 (S. DNA metabolism 3.1 cerevisiae) SETD4 SET domain containing 4 unknown 3.1

RRAGC Ras-related GTP binding C Autophagy 3.1

ATP6V1 C1 ATPase, H+ transporting, lysosomal 42kDa, V1 Lysosomal 3.1 subunit C1 acidification

PDP2 pyruvate dehydrogenase phosphatase isoenzyme 2 Mitochondrial 3.1 metabolism

HSPBAP1 HSPB (heat shock 27kDa) associated protein 1 unknown 3.1

SUNC1 Sad1 and UNC84 domain containing 1 unknown 3.1

ITPKB inositol 1 ,4,5-trisphosphate 3-kinase B Signal transduction 3.1

RPP25 ribonudease P/MRP 25kDa subunit RNA metabolism 3.0

CEP250 centrosomal protein 25OkDa Centrosome 3.0 component

TACC2 transforming, acidic coiled-coil containing protein 2 Centrosome 3.0 component

FAM83G family with sequence similarity 83, member G unknown 3.0

ATP6V1 B2 ATPase, H+ transporting, lysosomal 56/58kDa, V1 Lysosomal 3.0 subunit B2 acidification

PDE2A phosphodiesterase 2A, cGMP-stimulated Signal transduction 3.0

NSMCE2 non-SMC element 2, MMS21 homolog (S. DNA metabolism 3.0 cerevisiae)

WBP2 WW domain binding protein 2 Signal transduction 3.0

ATP6V0A1 ATPase, H+ transporting, lysosomal VO subunit a1 Lysosomal 3.0 acidification

LYPD3 LY6/PLAUR domain containing 3 unknown 3.0

CTSA cathepsin A Lysosomal hydrolase 3.0

MCCC1 methylcrotonoyl-Coenzyme A carboxylase 1 (alpha) Metabolism 3.0

ATP6V1 H ATPase, H+ transporting, lysosomal 50/57kDa, V1 Lysosomal 3.0 subunit H acidification

NR1 D2 nuclear receptor subfamily 1 , group D, member 2 Circadian rhythms 3.0

CLCN7 chloride channel 7 Lysosomal 3.0 acidification

RYBP RING1 and YY1 binding protein Transcription 3.0

LOC643338 hypothetical LOC643338 unknown 3.0

CLCN6 chloride channel 6 Endosomal 3.0 component

ZSCAN5A zinc finger and SCAN domain containing 5 Transcription 3.0

FOLR1 folate receptor 1 (adult) Metabolism 3.0

TRAF5 TNF receptor-associated factor 5 Apoptosis 3.0

HIF1A hypoxia-inducible factor 1 , alpha subunit (basic Transcription 3.0 helix-loop-helix transcription factor) PPP1 R13B protein phosphatase 1 , regulatory (inhibitor) subunit Apoptosis 3.0

13B GBA glucosidase, beta; acid (includes Lysosomal hydrolase 3.0 glucosylceramidase) ELOVL7 ELOVL family member 7, elongation of long chain Metabolism 3.0 fatty acids (yeast) TRPM7 transient receptor potential cation channel, Calcium ion transport 3.0 subfamily M, member 7 GLA galactosidase, alpha Lysosomal hydrolase 2.9

MAFF v-maf musculoaponeurotic fibrosarcoma oncogene Inflammatory 2.9 homolog F (avian) response UAP1 L1 UDP-N-acteylglucosamine pyrophosphorylase 1 -like Metabolism 2.9

1

ZNF330 zinc finger protein 330 unknown 2.9

PIP4K2C phosphatidylinositol-5-phosphate 4-kinase, type II, unknown 2.9 gamma

FNBP1 L formin binding protein 1-like Endocytosis 2.9

TNFAIP3 tumor necrosis factor, alpha-induced protein 3 Signal transduction 2.9

EPS8 epidermal growth factor receptor pathway substrate Signal transduction 2.9

8

PTGES prostaglandin E synthase Signal transduction 2.9

SCPEP1 serine carboxypeptidase 1 Lysosomal hydrolase 2.9

GTF2H1 general transcription factor NH, polypeptide 1 , Transcription 2.9

62kDa INSIG1 insulin induced gene 1 Cholesterol 2.9 metabolism

ARAP3 ArfGAP with RhoGAP domain, ankyrin repeat and Cytoskeleton 2.9

PH domain 3 component

TBC1 D14 TBC1 domain family, member 14 Signal transduction 2.9

KCNK9 potassium channel, subfamily K, member 9 Potassium ion 2.9 transport

TMCC3 transmembrane and coiled-coil domain family 3 unknown 2.9

AMPD3 adenosine monophosphate deaminase (isoform E) Metabolism 2.9

NAGPA N-acetylglucosamine-1-phosphodiester alpha-N- Lysosomal trafficking 2.9 acetylglucosaminidase GNS glucosamine (N-acetyl)-6-sulfatase (Sanfilippo Lysosomal hydrolase 2.9 disease IMD) TMEM38B transmembrane protein 38B Potassium ion 2.9 transport

SH3BP2 SH3-domain binding protein 2 Signal transduction 2.9

PMP22 peripheral myelin protein 22 Myelin component 2.9

TOB1 transducer of ERBB2, 1 Cell proliferation 2.9

GRAMD1 B GRAM domain containing 1 B unknown 2.8

ST3GAL4 ST3 beta-galactoside alpha-2,3-sialyltransferase 4 Golgi metabolism 2.8

NEU1 sialidase 1 (lysosomal sialidase) Lysosomal hydrolase 2.8

GNPDA1 glucosamine-6-phosphate deaminase 1 Golgi metabolism 2.8

TMEM55B transmembrane protein 55B Lysosomal 2.8 component BRI3 brain protein 13 Cell differentiation 2.8

C5orf24 hypothetical LOC134553 unknown 2.8

CYB5R1 cytochrome b5 reductase 1 Metabolism 2.8

TMEM159 transmembrane protein 159 unknown 2.8

GGA2 golgi associated, gamma adaptin ear containing, Golgi metabolism 2.8

ARF binding protein 2

RREB1 ras responsive element binding protein 1 Transcription 2.8

TRAPPC2L trafficking protein particle complex 2-like ER-Golgi transport 2.8

PCG F 1 polycomb group ring finger 1 Transcription 2.8

STK17B serine/threonine kinase 17b Apoptosis 2.8

MPHOSPH10 M-phase phosphoprotein 10 (U3 small nucleolar Ribosome biogenesis 2.8 ribonudeoprotein)

LOC440957 hypothetical LOC440957 unknown 2.8

CFB complement factor B Immune response 2.8

HTRA2 HtrA serine peptidase 2 Apoptosis 2.8

JPH1 junctophilin 1 unknown 2.8

SPG21 spastic paraplegia 21 (autosomal recessive, Mast Signal transduction 2.8 syndrome)

CCDC43 coiled-coil domain containing 43 unknown 2.8

ZCCHC8 zinc finger, CCHC domain containing 8 RNA metabolism 2.7

RAD9A RAD9 homolog A (S. pombe) DNA metabolism 2.7

GPR175 G protein-coupled receptor 175 Signal transduction 2.7

SNX8 sorting nexin 8 Transport 2.7

WDTC1 WD and tetratricopeptide repeats 1 unknown 2.7

AXUD1 AXIN1 up-regulated 1 unknown 2.7

PEA15 phosphoprotein enriched in astrocytes 15 Apoptosis 2.7

CD63 CD63 molecule Lysosomal 2.7 metabolism

SPNS1 spinster homolog 1 (Drosophila) unknown 2.7 LAMP1 lysosomal-associated membrane protein 1 Lysosomal 2.7 metabolism

C7orf20 chromosome 7 open reading frame 20 unknown 2.7

LAMB3 laminin, beta 3 Cell adhesion 2.7

PSAP prosaposin (variant Gaucher disease and variant Lysosomal hydrolase 2.7 metachromatic leukodystrophy) SNX27 sorting nexin family member 27 Endocytic trafficking 2.7

WIPH WD repeat domain, phosphoinositide interacting 1 Autophagy 2.7

ATP6V1 E1 ATPase, H+ transporting, lysosomal 31 kDa, V1 Lysosomal 2.6 subunit E1 acidification

CDKN1A cyclin-dependent kinase inhibitor 1A (p21 , Cip1) Cell cycle 2.6

C1orf85 chromosome 1 open reading frame 85 unknown 2.6

XRCC2 X-ray repair complementing defective repair in DNA metabolism 0.4 Chinese hamster cells 2 DDX58 DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 Immune response 0.4

BIRC3 baculoviral IAP repeat-containing 3 Apoptosis 0.4

DNAJB4 DnaJ (Hsp40) homolog, subfamily B, member 4 Stress response 0.3

LOC644714 hypothetical protein LOC644714 unknown 0.3

LCE2C late cornified envelope 2C Keratinization 0.3

LOC646993 similar to high-mobility group box 3 unknown 0.2

Table 4. Gene Ontology (GO) terms enriched within the set of genes upregulated following TFEB transient overexpression.

GO Term Gene Fold enr. P- value Count

Cellular Compartment GO:0005764~lysosome 17 8.2 2.50E-10 GO:0005765~lysosomal membrane 8 15.4 7.65E-07 GO:0005768~endosome 7 4.9 2.04E-04 GO:0048770~pigment granule 10 8.0 2.33E-04 GO:0042470~melanosome 7 8.0 2.33E-04 Biological Process GO:0015992~proton transport 7 7.0 4.87E-04 Molecular Function

GO:0022857~transmembrane transporter activity 27 2.5 1.78E-05 GO:0003824~catalytic activity 86 1.4 1.80E-04 GO:0019829~cation-transporting ATPase activity 6 10.3 2.75E-04

Table 5. Sequences of oligos used in real-time qPCR analysis

Gene name Forward primer Reverse primer

Expression analysis

TFEB CCAGAAGCGAGAGCTCACAGAT TGTGATTGTCTTTCTTCTGCCG

Seq Id No. 114 Seq Id No. 115

AGAGCTTTGCAGAGCGTTCAG ATACGCATGGTCTCAGGTCCA ARSA Seq Id No. 116 Seq Id No. 117

ATCAGTGAAGGAAGCCCATCC ACACGGTGAAGAGTCCACGAA ARSB Seq Id No. 118 Seq Id No. 119

CATTGTGATGAGCGTGTTCTGG AACTCCCCGGTTAGGACCCTTA ATP6V0E1 Seq Id No. 120 Seq Id No. 121

GGAAGTGTCAGATGATCCCCA CCGTTTGCCTCGTGGATAAT ATP6V1 H Seq Id No. 122 Seq Id No. 123

TGATCTCCACGTTCACCCTGA TCTCCGAGTCAAACCTTCCGA CLCN7 Seq Id No. 124 Seq Id No. 125

CAGGCTTTGGTCTTCTCTCCA TCACGCATTCCAGGTCTTTG CTSA Seq Id No. 126 Seq Id No. 127

CTSB AGTGGAGAATGGCACACCCTA AAGAAGCCATTGTCACCCCA Seq Id No. 128 Seq Id No. 129

AACTGCTGGACATCGCTTGCT CATTCTTCACGTAGGTGCTGGA CTSD Seq Id No. 130 Seq Id No. 131

ACAGAGGAGGAGTTCCGCACTA GCTTGCTTCATCTTGTTGCCA CTSF Seq Id No. 132 Seq Id No. 133

TTGTCGGCAAGTGGCATCT CCAAACCACTCATCAAATCCG GALNS Seq Id No. 134 Seq Id No. 135

TGGGTACCCGGATGATGTTA AGATGCTGCTGCTCTCAACA GBA Seq Id No. 136 Seq Id No. 137

AGCCAGATTCCTGCATCAGTG ATAACCTGCATCCTTCCAGCC GLA Seq Id No. 138 Seq Id No. 139

CCCATTTTGAGAGGTGCCAGT TGACGTTACGGCCTTCTCCTT GNS Seq Id No. 140 Seq Id No. 141

CAACCAACACATTCTTCTCCA CGCTATCGTGACCTGCTTTT HEXA Seq Id No. 142 Seq Id No. 143

ACGTTACAGCGTCCAGCTCAT TCTTTGGAGCTCGCATTGG LAMP1 Seq Id No. 144 Seq Id No. 145

TTGCTCTCTGCCAGCGGTACTA GCAGTCAGTAACCACCATCGGA MCOLN1 Seq Id No. 146 Seq Id No. 147

CAGAAGGAAGGAGCAGGAGT ATGTTCCCGAGGCTGTCAC NAGLU Seq Id No. 148 Seq Id No. 149

CAGCACATCCAGAGTTCCGAGT TGTCTCTTTCCGCCATGAGGT NEU1 Seq Id No. 150 Seq Id No. 151

GCCAACAGTGAAATCCCTTCC TCAGTGGCATTGTCCTTCAGC PSAP Seq Id No. 152 Seq Id No. 153

GATCTCCCCTGTTGATTCGGT AGCCCCTTATTTACGGCATTC SCPEP1 Seq Id No. 154 Seq Id No. 155

TGACCGGCCTTTCTTCCTCTA GCTCTCTCCGTTGCCAAACTT SGSH Seq Id No. 156 Seq Id No. 157

GTTCGATGCCCCTGTAACTGTC CCCAGGTTGATGATTCTTTTGC TMEM55B Seq Id No. 158 Seq Id No. 159

GATCCCAGCTCTCCTCAATACG GCCATTTTTGCACCGTGTG TPP1 Seq Id No. 160 Seq Id No. 161

TGCACCACCAACTGCTTAGC GGCATGGACTGTGGTCATGAG GAPDH Seq Id No. 162 Seq Id No. 163

TGACACTGGCAAAACAATGCA GGTCCTTTTCACCAGCAAGCT HPRT1 Seq Id No. 164 Seq Id No. 165

AGGAAACGGTTGCAGAAAGG GTCTTGCGGAGTGGGAATGT ARPP-19 Seq Id No. 166 Seq Id No. 167

ACTCACCGTGGTTGTTGGTAGA TCGATTGGTGGACTCTGGATAA C6orf211 Seq Id No. 168 Seq Id No. 169

GTGATGGACGAGGCCTTATTG TGCACATAAATCCCACCATGC FBXO11 Seq Id No. 170 Seq Id No. 171

HOXA9 CCCCCATCGATCCCAATAA CCCTGGTGAGGTACATGTTGAA Seq Id No. 172 Seq Id No. 173

TCCAAGCTACTCAAGCTGCCAG CCAGCCCGGATTATGTTGTCT KPNA2 Seq Id No. 174 Seq Id No. 175

CCTCTAAAACCCGTCCAAAACA TCGGTAGAAGTAGCAGGTGGAA MTDH Seq Id No. 176 Seq Id No. 177

TGCTGTTGACTGCAGAGCTGT CCTAGCATGAGTGATCTCCCCT MTX2 Seq Id No. 178 Seq Id No. 179

ATGTGGAAGTGGCTTCAGGAG GGGACTTCTTCTGGGAATTGT ONECUT2 Seq Id No. 180 Seq Id No. 181

GTCAGGTTGCTGGTCAAATTCC CAACGTCCCCAGAGTCTTTGTC STAT3 Seq Id No. 182 Seq Id No. 183

ChIP assay

TCGGGAATCCAGTTGTCCG GCCGCACAGGTAGAAGGAA ATP6V1 H Seq Id No. 184 Seq Id No. 185

CGTTGCAGGTCACATGGTC GGCTGCCCCCGTGTTTGT CLCN7 Seq Id No. 186 Seq Id No. 187

CCGTAGGGACCAAAGAAGG TGGAAGTCATGTGTACGAGTCA CTSA Seq Id No. 188 Seq Id No. 189

GCGTCATCCCGGCTATAAG TGAGGCTTCACCTGACGAG CTSD Seq Id No. 190 Seq Id No. 191

AAGCACGTGATAGAGGTCAGTG CCTGCGCGTTCTCTTGTT CTSF Seq Id No. 192 Seq Id No. 193

TGTAACAGATGAGAGGAAGC ACACAGGAAGTGAGGCAATC GBA Seq Id No. 194 Seq Id No. 195

TAGCGAGACGGTAGACGAC ACCCGCCCTATTTCCATAC GLA Seq Id No. 196 Seq Id No. 197

ATCGCGCCTAGGGAGAAA AATAAAAAGCCGTGCCTTGA GNS Seq Id No. 198 Seq Id No. 199

GTGAAAGGGCAGGGTGTG CGAATCACGTGACCAGAGG HEXA Seq Id No. 200 Seq Id No. 201

CTTCGAGATGCTGCGTGAT TCCCGGACTCTAATTGGTCTT NEU1 Seq Id No. 202 Seq Id No. 203

AGGGGCTCTGGGCTACC GCCCGCCGCTGTCACTG MCOLN1 Seq Id No. 204 Seq Id No. 205

TTGGGGCAGGGCAGATTTAT CAGGAGGAAGAGGGCGTACA PSAP Seq Id No. 206 Seq Id No. 207

CCGTCCGCCTCCGTCAC GGCAGCAGCAGCAACCAC SCPEP1 Seq Id No. 208 Seq Id No. 209

TCCCAATAGCTTGCAGAACC TGTCACATGACCTGCCAGA TMEM55B Seq Id No. 210 Seq Id No. 211

AGAGGGGTAGTGGTGGTGGAA CAGGCTTGGAGTCCCATTCT TPP1 Seq Id No. 212 Seq Id No. 213

CACAGGCACCCACACAAA ACGCAGCTGGTCAGCAAT PSAP(M) Seq Id No. 214 Seq Id No. 215 ACAAGGAATGGAAACAAAGATACC GATGCGTCTTCCCTTTTTCC GWS(int) Seq ld No. 216 Seq ld No. 217

ATGCAGCGATCAGTGGCGT TCCAGCTTCTTGTCACCACCTC ACTB Seq ld No. 218 Seq ld No. 219

GCCTTGACTCGCACTTTTGT TAGGCGCCATCGATTTTAAG APRT Seq Id No. 220 Seq Id No. 221

GCCACAGGTAGTGCAAGGTCTT TTCATGGCGGCCGTAAAC HPRT Seq Id No. 222 Seq Id No. 223

CCTCTCACACCCTCACTTCC TCTAGATGACGGACGACGTG TXNDC4 Seq Id No. 224 Seq Id No. 225

GCCAGCTTTGCCAGTCTTAC CGAGTCGCGCAAGAAGAT WIF1 Seq Id No. 226 Seq Id No. 227

Table 6. Positional weight matrix (PWM) describing CLEAR sequences (CLEAR PWM).

1 2 3 4 5 6 7 8 9 10

A 15 1 0 92 6 2 0 0 79 5

C 19 9 94 0 74 12 2 0 5 55

G 55 5 0 2 12 74 0 94 9 19

T 5 79 0 0 2 6 92 0 1 15

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