Title:
MONOCLONAL ANTIBODIES BINDING TO ORF8 PROTEINS OF SARS-COV-2 AND ANTI-INFLAMMATORY COMPOSITION CONTAINING SAID ANTIBODIES
Document Type and Number:
WIPO Patent Application WO/2023/068377
Kind Code:
A1
Abstract:
[Problem] Polyclonal antibodies or monoclonal antibodies that recognize ORF8 proteins are commercially available. However, the ORF8 proteins, which are antigens used in the acquisition of said polyclonal antibodies or monoclonal antibodies that recognize ORF8 proteins, did not have a dimer structure having three disulfide bonds in a molecule close to the native state and one disulfide bond between molecules. [Solution] The present invention has been completed by acquiring monoclonal antibodies using, as antigens, ORF8 proteins that have a dimer structure having three disulfide bonds in a molecule close to the native state and one disulfide bond between molecules, and by confirming that said antibodies have an anti-inflammatory effect.
Inventors:
MORI MASASHI (JP)
HIGASHIMURA YASUKI (JP)
IMAMURA TOMOHIRO (JP)
MIZUNO AKIKO (JP)
HIGASHIMURA YASUKI (JP)
IMAMURA TOMOHIRO (JP)
MIZUNO AKIKO (JP)
Application Number:
PCT/JP2022/039419
Publication Date:
April 27, 2023
Filing Date:
October 22, 2022
Export Citation:
Assignee:
ISHIKAWA PREFECTURAL UNIV (JP)
International Classes:
C12N15/13; A61K39/395; A61P29/00; A61P31/14; C07K14/165; C07K16/10; C07K16/46; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N7/00; C12N15/50
Other References:
ANONYMOUS: "SARS-CoV-2 Specificity Detects human SARS-CoV-2 ORF8 in direct ELISAs. Source Monoclonal Mouse IgG 2A Clone # 1041422", R&D SYSTEMS, 13 July 2021 (2021-07-13), pages 1 - 2, XP093057535, Retrieved from the Internet > [retrieved on 20230626]
WANG XIAOHUI, LAM JOY-YAN, CHEN LINLEI, AU SHANNON WING-NGOR, TO KELVIN K. W., YUEN KWOK-YUNG, KOK KIN-HANG: "Mining of linear B cell epitopes of SARS-CoV-2 ORF8 protein from COVID-19 patients", EMERGING MICROBES & INFECTIONS, vol. 10, no. 1, 1 January 2021 (2021-01-01), pages 1016 - 1023, XP093057536, DOI: 10.1080/22221751.2021.1931465
IMAMURA TOMOHIRO; ISOZUMI NORIYOSHI; HIGASHIMURA YASUKI; OHKI SHINYA; MORI MASASHI: "Production of ORF8 protein from SARS-CoV-2 using an inducible virus-mediated expression system in suspension-cultured tobacco BY-2 cells", PLANT CELL REPORTS, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 40, no. 3, 5 January 2021 (2021-01-05), Berlin/Heidelberg, pages 433 - 436, XP037366210, ISSN: 0721-7714, DOI: 10.1007/s00299-020-02654-5
LIN LIN XIAOYUAN XIAOYUAN, FU BEIBEI, YIN SONGNA, LI ZHIFENG, LIU HUAWEN, ZHANG HAIWEI, XING NA, WANG YU, XUE WEIWEI, XIONG YAN, Z: "ORF8 contributes to cytokine storm during SARS-CoV-2 infection by activating IL-17 pathway", ISCIENCE, ELSEVIER INC, UNITED STATES, 23 April 2021 (2021-04-23), United States, pages 102293 - 102293, XP093057545, Retrieved from the Internet [retrieved on 20230626], DOI: 10.1016/j.isci.2021.102293
WANG XIAOHUI, LAM JOY-YAN, CHEN LINLEI, AU SHANNON WING-NGOR, TO KELVIN K. W., YUEN KWOK-YUNG, KOK KIN-HANG: "Mining of linear B cell epitopes of SARS-CoV-2 ORF8 protein from COVID-19 patients", EMERGING MICROBES & INFECTIONS, vol. 10, no. 1, 1 January 2021 (2021-01-01), pages 1016 - 1023, XP093057536, DOI: 10.1080/22221751.2021.1931465
IMAMURA TOMOHIRO; ISOZUMI NORIYOSHI; HIGASHIMURA YASUKI; OHKI SHINYA; MORI MASASHI: "Production of ORF8 protein from SARS-CoV-2 using an inducible virus-mediated expression system in suspension-cultured tobacco BY-2 cells", PLANT CELL REPORTS, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 40, no. 3, 5 January 2021 (2021-01-05), Berlin/Heidelberg, pages 433 - 436, XP037366210, ISSN: 0721-7714, DOI: 10.1007/s00299-020-02654-5
LIN LIN XIAOYUAN XIAOYUAN, FU BEIBEI, YIN SONGNA, LI ZHIFENG, LIU HUAWEN, ZHANG HAIWEI, XING NA, WANG YU, XUE WEIWEI, XIONG YAN, Z: "ORF8 contributes to cytokine storm during SARS-CoV-2 infection by activating IL-17 pathway", ISCIENCE, ELSEVIER INC, UNITED STATES, 23 April 2021 (2021-04-23), United States, pages 102293 - 102293, XP093057545, Retrieved from the Internet
Attorney, Agent or Firm:
SHOJI Takashi et al. (JP)
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