Title:
MULTI-PURPOSE ACYLATION CATALAYST AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2007/066627
Kind Code:
A1
Abstract:
Disclosed are: a novel ribozyme system which can catalyze the acylation of tRNA using any one of various carboxylic acids as a donor of an acyl group; and use of the ribozyme system. A ribozyme capable of catalyzing the acylation of tRNA has a structure comprising an RNA sequence depicted by the formula (1): P1-Z1Z2Z3Z4(N1)1(N1)2···(N1)p-P2-(N2)1(N2)2···(N2)qY1Y2Y3 (N3)1(N3)2N4GGN [wherein (N1)1 to (N1)p independently represent any monoribonucleotide selected from U, C, A and G; p represents 3 or 4; (N2)1 to (N2)q independently represent any monoribonucleotide selected from U, C, A and G; q represents 5 or 6; (N3)1 to (N3)2 independently represent any monoribonucleotide selected from U, C, A and G; N4 represents any monoribonucleotide selected from U, C, A and G; Z1 to Z4 independently represent C or G; Y1 to Y3 independently represent C or G; N represents a monoribonucleotide complementary to A or G; and P1 and P2 independently represent an region comprising an RNA sequence which may have a stem loop structure.
Inventors:
SUGA HIROAKI (JP)
MURAKAMI HIROSHI (JP)
MURAKAMI HIROSHI (JP)
Application Number:
PCT/JP2006/324196
Publication Date:
June 14, 2007
Filing Date:
December 05, 2006
Export Citation:
Assignee:
UNIV TOKYO (JP)
SUGA HIROAKI (JP)
MURAKAMI HIROSHI (JP)
SUGA HIROAKI (JP)
MURAKAMI HIROSHI (JP)
International Classes:
C12N15/00; C12N9/00; C12N15/09; C12P21/02
Other References:
MURAKAMI H. ET AL.: "A versatile tRNA aminoacylation catalyst based on RNA", CHEM. BIOL., vol. 10, no. 7, 2003, pages 655 - 662, XP003013894
MURAKAMI H. ET AL.: "Using a solid-phase ribozyme aminoacylation system to reprogram the genetic code", CHEM. BIOL., vol. 10, no. 11, 2003, pages 1077 - 1084, XP003013895
MURAKAMI H. ET AL.: "Jinko Ribozyme no Shinka to Iden Ango Kaihen eno Oyo", 28TH ANNUAL MEETING OF THE MOLECULAR BIOLOGY SOCIETY OF JAPAN KOEN YOSHISHU, 25 November 2005 (2005-11-25), pages 129, YG4H-1, XP003013896
MURAKAMI H. ET AL.: "A highly flexible tRNA acylation method for non-natural polypeptide synthesis", NAT. METHODS, vol. 3, no. 5, May 2006 (2006-05-01), pages 357 - 359, XP003013897
MURAKAMI H. ET AL.: "Seimei o Design suru Gosei Seibutsugaku Central Dogma o Tukuri Kaeru", BIONICS, vol. 3, no. 3, 1 March 2006 (2006-03-01), pages 34 - 39, XP003013898
MURAKAMI H. ET AL.: "Flexizyme System: mRNA o Igata to shita Hitennen Peptide no Gosei eno Oyo", CSJ: THE CHEMICAL SOCIETY OF JAPAN KOEN YOKOSHU, vol. 86TH, no. 2, 13 March 2006 (2006-03-13), pages 895, 3 G4-43, XP003013899
See also references of EP 1964916A4
MURAKAMI H. ET AL.: "Using a solid-phase ribozyme aminoacylation system to reprogram the genetic code", CHEM. BIOL., vol. 10, no. 11, 2003, pages 1077 - 1084, XP003013895
MURAKAMI H. ET AL.: "Jinko Ribozyme no Shinka to Iden Ango Kaihen eno Oyo", 28TH ANNUAL MEETING OF THE MOLECULAR BIOLOGY SOCIETY OF JAPAN KOEN YOSHISHU, 25 November 2005 (2005-11-25), pages 129, YG4H-1, XP003013896
MURAKAMI H. ET AL.: "A highly flexible tRNA acylation method for non-natural polypeptide synthesis", NAT. METHODS, vol. 3, no. 5, May 2006 (2006-05-01), pages 357 - 359, XP003013897
MURAKAMI H. ET AL.: "Seimei o Design suru Gosei Seibutsugaku Central Dogma o Tukuri Kaeru", BIONICS, vol. 3, no. 3, 1 March 2006 (2006-03-01), pages 34 - 39, XP003013898
MURAKAMI H. ET AL.: "Flexizyme System: mRNA o Igata to shita Hitennen Peptide no Gosei eno Oyo", CSJ: THE CHEMICAL SOCIETY OF JAPAN KOEN YOKOSHU, vol. 86TH, no. 2, 13 March 2006 (2006-03-13), pages 895, 3 G4-43, XP003013899
See also references of EP 1964916A4
Attorney, Agent or Firm:
SHAMOTO, Ichio et al. (Section 206 Mew Ohtemachi Bldg., 2-1, Ohtemachi 2-chom, Chiyoda-ku Tokyo 04, JP)
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