Title:
MULTIMER TYPE DISCRIMINATION AND DETECTION METHOD FOR MULTIMER-FORMING POLYPEPTIDE
Document Type and Number:
WIPO Patent Application WO/2013/103245
Kind Code:
A1
Abstract:
The present invention relates to a method for selectively detecting a multimer type multimer-forming polypeptide in a biological sample, the method comprising: (a) bringing the biological sample into contact with an agglutination reaction inducing agent to induce the formation of an aggregate in an analysis target, the agglutination reaction inducing agent being a particle in which a specific antibody is surface-bonded with the multimer-forming polypeptide; (b) obtaining an image with respect to the aggregate of step (a); and (c) analyzing a size or a shape of the aggregate by using the image. Step (a), step (b), or steps (a) and (b) are performed on a microchip having a microchannel. The image analysis is performed using a coefficient according to the size of the aggregate in a predetermined volume provided by the microchannel. In the case where the multimer type of multimer-forming polypeptide is present in the biological sample, the size of the aggregate is larger than the size of an aggregate of a monomer type control group. According to the present invention, unlike in a detection method using chemiluminescence immunoanalysis of the related art, an image with respect to an agglutination reaction target is obtained and then a size or a shape of an aggregate is analyzed so as to determine whether or not an analysis target is present in a biological sample and to determine the quantity of the analysis target. Also, it is possible to detect a multimer type multimer-forming polypeptide by just analyzing an image acquired from the sample so that the detection process is made more convenient and quick.
Inventors:
CHUNG CHAN IL (KR)
HUR DAE SUNG (KR)
KIM HO YOUNG (KR)
KIM JASON SUNG (US)
HUR DAE SUNG (KR)
KIM HO YOUNG (KR)
KIM JASON SUNG (US)
Application Number:
PCT/KR2013/000025
Publication Date:
July 11, 2013
Filing Date:
January 03, 2013
Export Citation:
Assignee:
NANOENTEK INC (KR)
International Classes:
G01N33/68; G01N33/52; G01N33/532
Domestic Patent References:
| WO2000043791A2 | 2000-07-27 | |||
| WO2011049530A1 | 2011-04-28 |
Foreign References:
| KR20080000566A | 2008-01-02 | |||
| US20060211071A1 | 2006-09-21 | |||
| KR20090013186A | 2009-02-04 | |||
| US20070077603A1 | 2007-04-05 |
Attorney, Agent or Firm:
YANG, Boo-Hyun (Chungdong Building1922, Nambusunhwan-r, Gwanak-gu Seoul 151-832, KR)
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Claims: